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Correction to: Protein Cell (2021) https://doi.org/10.1007/s13238-021-00837-8
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YTHDC1 is essential for mouse ESCs. (A) Strategy for functional studies of Ythdc1 in ESCs. All cell lines were treated with 4-OHT for 3 days before harvest to ensure the depletion of endogenous Ythdc1. (B) Schematic of mouse wild-type (WT) YTHDC1, truncated YTHDC1 after the recombination (KO YTHDC1) and mutant YTHDC1 (W378A YTHDC1). aa, amino acid. (C) Growth curve showing that Ythdc1 cKO and W378A ESCs exhibited a poor proliferation rate. Cell numbers on the last day were used to assess the significance. (D and E) Colony formation abilities of Ythdc1 cKO and W378A ESCs were impaired revealed by AP staining. (F) RT-qPCR analysis showing the relative RNA level of key pluripotent markers in Ythdc1 f/f and cKO ESCs. (G) RT-qPCR analysis showing that EBs derived from Ythdc1 cKO ESCs exhibited the abnormal expression level of differentiation markers 7 days after in vitro differentiation. (H) Ythdc1 cKO and W378A ESCs exhibited a weak ability to generate chimeric mice. (I) Principal component analysis (PCA) showing the transcriptome differences between each ESC line. (J) GO analysis of genes dysregulated in both Ythdc1 cKO and W378A ESCs defined in Fig. S2E. Fold enrichment of each term is labeled in the plot. Data are presented as means with SDs (n = 3 in (C, F and G) and n = 4 in (D). Significance was calculated with unpaired two-tailed Student’s t test (**P < 0.01, ***P < 0.001, ****P < 0.0001). See also Figs. S1 and S2
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Chen, C., Liu, W., Guo, J. et al. Correction to: Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos. Protein Cell 13, 470–471 (2022). https://doi.org/10.1007/s13238-021-00853-8
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DOI: https://doi.org/10.1007/s13238-021-00853-8