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Ecotoxicological studies on fish, Labeo rohita exposed to tannery industry effluent by using micronucleus test

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Abstract

Tannery industries are globally situated. The genotoxic effect was analyzed in erythrocytes of a freshwater fish, Labeo rohita exposed to tannery industry effluent using micronucleus test. Micronucleus test is simple, rapid and reliable method used in laboratory for monitoring genotoxic damage. The 96 h LC50 value was calculated by using Finney method and it came out to be 7.07 %. Fishes were exposed to three sub-lethal concentrations (3.53, 1.76 and 0.88 % of 96 h LC50 value) for 24, 48, 72 and 96 h. Presence of one micronucleus was predominant than two and three to five micronuclei in erythrocytes of fishes exposed to effluent. Concentration 3.53 % proved to be highly toxic and 0.88 % can act as a safe disposal concentration. The results showed concentration and time dependent response. Thus, results revealed that discharge of tannery effluent caused clastogenic effects in fish erythrocytes. This may lead to stock depletion of fishes and aquatic organisms living in rivers. Similar clastogenic effects may also occur in human population living around rivers using contaminated water and consuming contaminated fishes.

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Acknowledgments

Authors would like to thank the Department of Zoology and Environmental Sciences, Punjabi University, Patiala for extending help and support in the form of infrastructure to carry out the research work smoothly.

Author’s contributions

Diana Handa carried out all the work of collection of effluent, fishes and experimental part with the help of Rohit Kalotra (Co-author), Gurinder Kaur Walia and Dr. Harbhajan Kaur have been involved in drafting the manuscript for its content.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Zoology and Environmental Sciences, Punjabi University, Patiala, 147002, Punjab, India

    Gurinder Kaur Walia, Diana Handa, Harbhajan Kaur & Rohit Kalotra

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  1. Gurinder Kaur Walia
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  2. Diana Handa
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  3. Harbhajan Kaur
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  4. Rohit Kalotra
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Correspondence to Gurinder Kaur Walia.

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Walia, G.K., Handa, D., Kaur, H. et al. Ecotoxicological studies on fish, Labeo rohita exposed to tannery industry effluent by using micronucleus test. Nucleus 58, 111–116 (2015). https://doi.org/10.1007/s13237-015-0140-5

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