Skip to main content
SpringerLink
Log in

Faecal Microbiota Microsphere Contributed to Relieving Gut Barrier Damage in Colitis

  • Article
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

Gut microbiota play an important role in many diseases. However, few researches evaluated the effect of faecal bacteria transplantation in protecting gut barrier during colitis. In this study, we focused on the effect of faecal microbiota encapsulated alginate microsphere on dextran sulfate sodium (DSS) induced colitis mice. Bacteria are sensitive to gastric juice and digestive enzymes, which is the dilemma of utilizing microbiota. Through taking advantage of alginate, microspheres that could protect its contents were fabricated. In vitro drug release experiment demonstrated their ability in protecting contents in simulated gastric fluid and release contents in simulated intestinal fluid. Thus, the resultant microsphere could be used in faecal microbiota transplantation. In vivo, the transplantation of faecal microbiota from healthy mice showed protection against DSS induced oxidative stress and gut barrier damage. Our findings suggest that the faecal microbiota transplantation could be a potential effective treatment for protecting gut barrier in inflammatory bowel diseases (IBD) colitis with faecal microbiota encapsulated alginate microsphere.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. Le Berre, A. N. Ananthakrishnan, S. Danese, S. Singh, and L. Peyrin-Biroulet, Clin. Gastroenterol. Hepatol., 18, 14 (2019).

    PubMed  Google Scholar 

  2. P. Olivera, S. Danese, N. Jay, G. Natoli, and L. Peyrin-Biroulet, Nat. Rev. Gastroenterol. Hepatol., 16, 312 (2019).

    PubMed  Google Scholar 

  3. A. F. Peery, S. D. Crockett, C. C. Murphy, J. L. Lund, E. S. Dellon, J. L. Williams, E. T. Jensen, N. J. Shaheen, A. S. Barritt, S. R. Lieber, B. Kochar, E. L. Barnes, Y. C. Fan, V. Pate, J. Galanko, T. H. Baron, and R. S. Sandler, Gastroenterology, 156, 254 (2019).

    PubMed  Google Scholar 

  4. A. Yarlas, D. T. Rubin, J. Panés, J. O. Lindsay, S. Vermeire, M. Bayliss, J. C. Cappelleri, S. Maher, A. G. Bushmakin, L. A. Chen, and M. DiBonaventura, J. Crohn’s Colitis, 12, 600 (2018).

    Google Scholar 

  5. S. E. Kim, Intest. Res., 17, 443 (2019).

    PubMed  PubMed Central  Google Scholar 

  6. L. T. Marton, R. A. Goulart, A. C. A. Carvalho, and S. M. Barbalho, Int. J. Mol. Sci., 20, 4851 (2019).

    CAS  PubMed Central  Google Scholar 

  7. Y. Mishima and R. B. Sartor, J. Gastroenterol., 55, 4 (2020).

    PubMed  Google Scholar 

  8. S. Picardo, G. G. Kaplan, K. A. Sharkey, and C. H. Seow, Therap. Adv. Gastroenterol., 12, 1756284819870977 (2019).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. K. Matsuoka and T. Kanai, Semin. Immunopathol., 37, 47 (2015).

    CAS  PubMed  Google Scholar 

  10. R. B. Sartor and G. D. Wu, Gastroenterology, 152, 327 (2017).

    CAS  PubMed  Google Scholar 

  11. A. R. Weingarden and B. P. Vaughn, Gut Microbes, 8, 238 (2017).

    PubMed  PubMed Central  Google Scholar 

  12. C. Zhao, Q. Hu, X. Wu, G. Chen, H. Wu, and J. Ren, Macromol. Res., 27, 888 (2019).

    CAS  Google Scholar 

  13. B. P. Abraham and E. M. M. Quigley, Gastroenterol. Clin. North Am., 46, 769 (2017).

    PubMed  Google Scholar 

  14. D. Curro, G. Ianiro, S. Pecere, S. Bibbo, and G. Cammarota, Br. J. Pharmacol., 174, 1426 (2017).

    CAS  PubMed  Google Scholar 

  15. M. N. Quraishi, M. Widlak, N. Bhala, D. Moore, M. Price, N. Sharma, and T. H. Iqbal, Aliment. Pharmacol. Ther., 46, 479 (2017).

    CAS  PubMed  Google Scholar 

  16. W. K. Smits, D. Lyras, D. B. Lacy, M. H. Wilcox, and E. J. Kuijper, Nat. Rev Dis. Primers, 2, 16020 (2016).

    PubMed  PubMed Central  Google Scholar 

  17. J. Halfvarson, C. J. Brislawn, R. Lamendella, Y. Vázquez-Baeza, W. A. Walters, L. M. Bramer, M. D’Amato, F. Bonfiglio, D. McDonald, A. Gonzalez, E. E. McClure, M. F. Dunklebarger, R. Knight, and J. K. Jansson, Nat. Microbiol., 2, 17004 (2017).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. S. Paramsothy, R. Paramsothy, D. T. Rubin, M. A. Kamm, N. O. Kaakoush, H. M. Mitchell, and N. Castaño-Rodríguez, J. Crohn’s Colitis, 11, 1180 (2017).

    Google Scholar 

  19. X. Ge, C. Ding, J. Gong, H. Tian, Y. Wei, Q. Chen, L. Gu, and N. Li, Zhonghua wei chang wai ke za zhi = Chinese Journal of Gastrointestinal Surgery, 19, 1355 (2016).

    PubMed  Google Scholar 

  20. X. Ge, C. Ding, W. Zhao, L. Xu, H. Tian, J. Gong, M. Zhu, J. Li, and N. Li, J. Transl. Med., 15, 13 (2017).

    PubMed  PubMed Central  Google Scholar 

  21. X. Ge, W. Zhao, C. Ding, H. Tian, L. Xu, H. Wang, L. Ni, J. Jiang, J. Gong, W. Zhu, M. Zhu, and N. Li, Sci. Rep., 7, 441 (2017).

    PubMed  PubMed Central  Google Scholar 

  22. Y. Q. Qiao, C. W. Cai, and Z. H. Ran, J. Dig. Dis., 17, 800 (2016).

    PubMed  Google Scholar 

  23. R. Caillard and N. Lapointe, Int. J. Pharm., 519, 125 (2017).

    CAS  PubMed  Google Scholar 

  24. I. Trabelsi, W. Bejar, D. Ayadi, H. Chouayekh, R. Kammoun, S. Bejar, and R. Ben Salah, Int. J. Biol. Macromol., 61, 36 (2013).

    CAS  PubMed  Google Scholar 

  25. S. H. Ching, N. Bansal, and B. Bhandari, Crit. Rev. Food Sci. Nutr., 57, 1133 (2017).

    CAS  PubMed  Google Scholar 

  26. K. Y. Lee and D. J. Mooney, Prog. Polym. Sci., 37, 106 (2012).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. S. N. Pawar and K. J. Edgar, Biomaterials, 33, 3279 (2012).

    CAS  PubMed  Google Scholar 

  28. C. Zhao, Y. Yu, X. Zhang, X. Wu, J. Ren, and Y. Zhao, Sci. Bull., 64, 1418 (2019).

    Google Scholar 

  29. M. Chávarri, I. Marañón, R. Ares, F. C. Ibáñez, F. Marzo, and M. del Carmen Villarán, Int. J. Food Microbiol., 142, 185 (2010).

    PubMed  Google Scholar 

  30. M. Gou, K. Men, H. Shi, M. Xiang, J. Zhang, J. Song, J. Long, Y. Wan, F. Luo, X. Zhao, and Z. Qian, Nanoscale, 3, 1558 (2011).

    CAS  PubMed  Google Scholar 

  31. C. Zhao, X. Wu, G. Chen, F. Wang, and J. Ren, J. Biomater. Sci. Polym. Ed., 29, 2035 (2018).

    CAS  PubMed  Google Scholar 

  32. C. Zhao, X. Wu, J. Huang, C. Chen, J. Yu, M. Fang, G. Wang, and J. Ren, J. Mater. Sci.-Mater. Med., 30, 109 (2019).

    PubMed  Google Scholar 

  33. T. K. Dash and V. B. Konkimalla, J. Control. Release, 158, 15 (2012).

    CAS  PubMed  Google Scholar 

  34. N. V. N. Jyothi, P. M. Prasanna, S. N. Sakarkar, K. S. Prabha, P. S. Ramaiah, and G. Y. Srawan, J. Microencapsul., 27, 187 (2010).

    CAS  PubMed  Google Scholar 

  35. S. Mitragotri, P. A. Burke, and R. Langer, Nat. Rev. Drug Discov., 13, 655 (2014).

    CAS  PubMed  PubMed Central  Google Scholar 

  36. J. F. Liu, B. Jang, D. Issadore, and A. Tsourkas, Wiley Interdiscip Rev Nanomed Nanobiotechnol., 11, e1571 (2019).

    PubMed  Google Scholar 

  37. A. Puiggali-Jou, L. J. Del Valle, and C. Aleman, J. Control. Release, 309, 244 (2019).

    CAS  PubMed  Google Scholar 

  38. L. Aguero, D. Zaldivar-Silva, L. Pena, and M. L. Dias, Carbohydr. Polym., 168, 32 (2017).

    CAS  PubMed  Google Scholar 

  39. D. Dhamecha, R. Movsas, U. Sano, and J. U. Menon, Int. J. Pharm., 569, 118627 (2019).

    CAS  PubMed  Google Scholar 

  40. G. Aviello and U. G. Knaus, Mucosal Immunol., 11, 1011 (2018).

    CAS  PubMed  Google Scholar 

  41. E. L. Campbell and S. P. Colgan, Nat. Rev. Gastroenterol. Hepatol., 16, 106 (2019).

    PubMed  Google Scholar 

  42. L. Dumitrescu, I. Popescu-Olaru, L. Cozma, D. Tulbă, M. E. Hinescu, L. C. Ceafalan, M. Gherghiceanu, and B. O. Popescu, Oxid. Med. Cell. Longev., 2018, 2406594 (2018).

    PubMed  PubMed Central  Google Scholar 

  43. E. C. Vasquez, T. M. C. Pereira, V. A. Peotta, M P. Baldo and M. Campos-Toimil, Oxid. Med. Cell Longev., 2019, 3086270 (2019).

    PubMed  PubMed Central  Google Scholar 

Download references

Author information

Author notes

  1. S. Zhen and C. Zhao contributed equally to this work.

Authors and Affiliations

  1. Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, P. R. China

    Shuang Zhen, Wu Ji & Jieshou Li

  2. Department of Endocrinology, Xiangya Shenzhen Endocrinology and Metabolism Center, The First Affiliated Hospital of Shenzhen University, Shenzhen, P. R. China

    Cheng Zhao

  3. Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P. R. China

    Xin Zhao

Authors
  1. Shuang Zhen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cheng Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wu Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jieshou Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wu Ji.

Additional information

Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Acknowledgments: This work was supported by the The General Program of Jiangsu Province (Grant Numbers: BK20171329) and China Postdoctoral Science Foundation Grant (Numbers: 2019M663108).

Supporting Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhen, S., Zhao, C., Zhao, X. et al. Faecal Microbiota Microsphere Contributed to Relieving Gut Barrier Damage in Colitis. Macromol. Res. 28, 644–649 (2020). https://doi.org/10.1007/s13233-020-8078-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-020-8078-0

Keywords

Search

Navigation