Skip to main content
SpringerLink
Log in

Alginate and Probiotics Synergistically Reversed Dextran Sulfate Sodium Salt (DSS)-Induced Gut Barrier Damage

  • Article
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

Previous studies have established that sole probiotics or fibre could be used to relieve colitis. However, few studies evaluated the synergistical effect of fibre and probiotics in treating colitis. This study, examined the effect of alginate, one of the commonest biomaterials and dietary fibre, and/or probiotics on the gut barrier in colitis mice. A synergistical effect was found between alginate and probiotics in colitis. Dextran sulfate sodium salt (DSS), a chemical toxic substance, was used to induce colitis model. Body weight, oxidative stress, inflammatory factors, gut barrier, intestinal morphology and the apoptosis of intestine epithelial cells were analyzed. A combination of probiotics and alginate was used to analyze their synergistic effect. The results showed that the combination of alginate and probiotics had a better therapeutic effect on colitis than using them individually, and they protected the gut barrier from DSS induced oxidative stress. The combination exhibited a greater ability in reducing pro-inflammatory factors and protecting the gut barrier from further damage. The synergistic effect of alginate and probiotics provides a great prospect for biotherapy in bowel diseases and oral drug delivery system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. N. Ananthakrishnan, Nat. Rev. Gastro. Hepat., 12, 205 (2015).

    Article  Google Scholar 

  2. N. A. Molodecky, I. S. Soon, D. M. Rabi, W. A. Ghali, M. Ferris, G. Chern-off, E. I. Benchimol, R. Panaccione, S. Ghosh, H. W. Barkema, and G. G. Kaplan, Gastroenterology, 142, 46 (2012).

    PubMed  Google Scholar 

  3. B. Khor, A. Gardet, and R. J. Xavier, Nature, 474, 307 (2011).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. M. L. Harris, H. J. Schiller, P. M. Reilly, M. Donowitz, M. B. Grisham, G. B. Bulkley, Pharmacol. Therapeut., 53, 375 (1992).

    Article  CAS  Google Scholar 

  5. R. G. Strickland and D. P. Jewell, Annu. Rev. Med, 34, 195-(1983).

    Article  CAS  PubMed  Google Scholar 

  6. F. Sommer, J. M. Anderson, R. Bharti, J. Raes, and P. Rosenstiel, Nat. Rev. Microbiol, 15, 630 (2017).

    Article  CAS  PubMed  Google Scholar 

  7. T. S. Postler and S. Ghosh, Cell Metab, 26, 110 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. A. B. Hall, A. C. Tolonen, and R. J. Xavier, Nat Rev. Genet., 18, 690 (2017).

    Article  CAS  PubMed  Google Scholar 

  9. J. M. Blander, R. S. Longman, I. D. Iliev, G. F. Sonnenberg, and D. Artis, Nat Immunol, 18, 851 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. M. Chichlowski and L. P. Hale, Am. J. Physiol-Gastr. L, 295, G1139 (2008).

    CAS  Google Scholar 

  11. F. Liu, J. J. Cottrell, J. B. Furness, L. R. Rivera, F. W. Kelly, U. Wijesiri-wardana, R. V. Pustovit L. J. Fothergill, D. M. Bravo, P. Celi, B. J. Leury, N. K. Gabler, F. R. Dunshea, Exp. Physiol., 101, 801 (2016).

    Article  CAS  PubMed  Google Scholar 

  12. C. Yu, Z. Wang, S. Tan, Q. Wang, C. Zhou, X. Kang, S. Zhao, S. Liu, H. Fu, Z. Yu, and A. Peng, Gastroent Res. Pract., 2016, 6720575 (2016).

    Google Scholar 

  13. M. Ganji-Arjenaki and M. Rafieian-Kopaei, J. Cell. Physiol., 233, 2091 (2018).

    Article  CAS  PubMed  Google Scholar 

  14. H. Zheng, M. Gao, Y. Ren, R. Lou, H. Xie, W. Yu, X. Liu, and X. Ma, Carbohyd. Polym., 155, 329 (2017).

    Article  CAS  Google Scholar 

  15. S. Akay, R. Heils, H. K. Trieu, I. Smirnova, and O. Yesil-Celiktas, Carbohyd. Polym., 161, 228 (2017).

    Article  CAS  Google Scholar 

  16. S. R. Caliari and J. A. Burdick, Nat Methods, 13, 405 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. J. Zhang, A. D. Holdorf, and A. J. Walhout, Curr. Opin. Biotechnol., 46, 74 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. F. Shanahan, D. van Sinderen, P. W. O’Toole, and C. Stanton, Gut, 66, 1709 (2017).

    Article  CAS  PubMed  Google Scholar 

  19. N. D. Schwalm, 3rd and E. A. Groisman, Trends Microbiol., 25, 1005 (2017).

    Article  CAS  PubMed  Google Scholar 

  20. J. Chilloux and M. E. Dumas, Cell Metab., 25, 6 (2017).

    Article  CAS  PubMed  Google Scholar 

  21. T. Yamamoto, T. Shimoyama, and M. Kuriyama, Curr. Opin. Biotechnol., 44, 69 (2017).

    Article  CAS  PubMed  Google Scholar 

  22. G. S. Hotamisligil, Nature, 542, 177 (2017).

    Article  CAS  PubMed  Google Scholar 

  23. F. Shanahan and D. Sheehan, Curr. Opin. Clin. Nutr, 19, 257 (2016).

    Article  CAS  Google Scholar 

  24. D. L. Merrifield, G. M. Harper, S. Mustafa, O. Carnevali, S. Picchietti, S. J. Davies, Cell Tissue Res, 344, 135 (2011).

    Article  CAS  PubMed  Google Scholar 

  25. G. Ashouri, N. Mahboobi Soofiani, S. H. Hoseinifar, S. A. H. Jalali, V. Morshedi, H. Van Doan, and M. Torfi Mozanzadeh, Fish Shellfish Immun., 79, 34 (2018).

    Article  CAS  Google Scholar 

  26. E. Wang, X. Wang, K. Wang, J. He, L. Zhu, Y. He, D. Chen, P. Ouyang, Y. Geng, X. Huang, and W. Lai, Fish Shellfish Immun., 73, 262 (2018).

    Article  CAS  Google Scholar 

  27. I. Leake, Nat Rev. Gastro. Hepat., 10, 386 (2013).

    Article  Google Scholar 

  28. W. O. Rohof, R. J. Bennink, A. J. Smout, E. Thomas, G. E. Boeckxstaens, Clin. Gastroenterol. H, 11, 1585 (2013).

    Article  CAS  Google Scholar 

  29. A. Yamamoto, T. Itoh, R. Nasu, and R. Nishida, World J. Gastroentero, 20, 2641 (2014).

    Article  CAS  Google Scholar 

  30. A. Costabile, T. Bergillos-Meca, P. Rasinkangas, K. Korpela, W. M. de Vos, and G. R. Gibson, Front. Immunol., 8, 1443 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  31. C. McKenzie, J. Tan, L. Macia, and C. R. Mackay, Immunol. Rev, 278, 277 (2017).

    Article  CAS  PubMed  Google Scholar 

  32. C. M. van der Beek, C. H. C. Dejong, F. J. Troost, A. A. M. Masclee, and K. Lenaerts, Nutr. Rev., 75, 286 (2017).

    Article  PubMed  Google Scholar 

  33. L. Chen, Z. He, A. C. Iuga, S. N. Martins Filho, J. J. Faith, J. C. Clemente, M. Deshpande, A. Jayaprakash, J. F. Colombel, J. J. Lafaille, R. Sachidanandam, G. C. Furtado, and S. A. Lira, Gastroenterology, 155, 1177 (2018).

    Article  CAS  PubMed  Google Scholar 

  34. C. Amico, T. Tornetta, C. Scifo, and A. R. Blanco, Curr. Eye Res., 40, 72 (2015).

    Article  CAS  PubMed  Google Scholar 

  35. A. Bermudez-Oria, G. Rodriguez-Gutierrez, E. Rodriguez-Juan, A. Gonzalez-Benjumea, and J. Fernandez-Bolanos, Carbohyd. Polym., 197, 260 (2018).

    Article  CAS  Google Scholar 

  36. Q. Chen, X. Shao, P. Ling, F. Liu, H. Shao, A. Ma, J. Wu, W. Zhang, F. Liu, G. Han, and F. Wang, Carbohyd. Polym., 169, 255 (2017).

    Article  CAS  Google Scholar 

  37. L. Xing, T.-J. Zhou, Y.-T. Fan, Y.-J. He, T. Pang, K.-H. Cho, J.-J. Lu, H.-L. Jiang, and C.-S. Cho, Macromol. Res., 27, 215 (2019).

    Article  CAS  Google Scholar 

  38. H.-J. Hong, H. S. Jeong, K.-M. Roh, and I. Kang, Macromol. Res., 26, 1019 (2018).

    Article  CAS  Google Scholar 

  39. V. Singh, B. S. Yeoh, B. Chassaing, X. Xiao, P. Saha, R. Aguilera Olvera, J. D. Lapek, Jr., L. Zhang, W. B. Wang, S. Hao, M. D. Flythe, D. J. Gonzalez, P. D. Cani, J. R. Conejo-Garcia, N. Xiong, M. J. Kennett, B. Joe, A. D. Patterson, A. T. Gewirtz, and M. Vijay-Kumar, Cell, 175, 679 (2018).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. L. Zhao, F. Zhang, X. Ding, G. Wu, Y. Y. Lam, X. Wang, H. Fu, X. Xue, C. Lu, J. Ma, L. Yu, C. Xu, Z. Ren, Y. Xu, S. Xu, H. Shen, X. Zhu, Y. Shi, Q. Shen, W. Dong, R. Liu, Y. Ling, Y. Zeng, X. Wang, Q. Zhang, J. Wang, L. Wang, Y. Wu, B. Zeng H. Wei, M. Zhang, Y. Peng, and C. Zhang, Science (New York, N. Y.) 359, 1151 (2018).

    Article  CAS  Google Scholar 

  41. C.-M. Lee, H.-J. Jeong, D.-W. Kim, and K.-Y. Lee, Macromol. Res., 16, 429 (2008).

    Article  CAS  Google Scholar 

  42. H.-J. Hong, J. Kim, Y. J. Suh, D. Kim, K.-M. Roh, and I. Kang, Macromol. Res., 25, 1145 (2017).

    Article  CAS  Google Scholar 

  43. S. N. Pratt and D. E. Austin, J. Am. Soc. Mass Spectr., 25, 712 (2014).

    Article  CAS  Google Scholar 

  44. S. Zhao, P. Agarwal, W. Rao, H. Huang R. Zhang, Z. Liu, J. Yu, N. Weisleder, W. Zhang, and X. He, Integr. Biol., 6, 874 (2014).

    Article  CAS  Google Scholar 

  45. S. Khorasani, M. Mahmoudi, M. R. Kalantari, F. Lavi Arab, S. A. Esmaeili, F. Mardani, N. Tabasi, and M. Rastin, J. Cell. Physiol., 234, 9778 (2019).

    Article  CAS  PubMed  Google Scholar 

  46. P. K. Deol, P. Khare, D. P. Singh, G. Soman, M. Bishnoi, K. K. Kondepudi, and I. P. Kaur, Int J. Biol. Macromol., 105, 81 (2017).

    Article  CAS  PubMed  Google Scholar 

  47. N. Varankovich, A. Grigoryan, K. Brown, G. D. Inglis, R. R. E. Uwiera, M. T. Nickerson, and D. R. Korber, Can. J. Microbiol., 64, 744 (2018).

    Article  CAS  PubMed  Google Scholar 

  48. A. Yamamoto, T. Itoh, R. Nasu, and R. Nishida, Pharmacology, 92, 108 (2013).

    Article  CAS  PubMed  Google Scholar 

  49. C. Kilkenny, W. J. Browne, I. C. Cuthill, M. Emerson, and D. G. Altman, J. Pharmacol. Pharmacother., 1, 94 (2010).

    Article  PubMed  PubMed Central  Google Scholar 

  50. H. Tan, W. Chen, Q. Liu, G. Yang, and K. Li, Front. Immunol., 9, 1504 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  51. O. J. Lara-Guzman, A. Gil-Izquierdo, S. Medina, E. Osorio, R. Alvarez-Quintero, N. Zuluaga, C. Oger, J. M. Galano, T. Durand, K. Munoz-Durango, Redox Biology 15, 1 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  52. V. Mishra, J. Banga, and P. Silveyra, Pharmacol. Ther, 181, 169 (2018).

    Article  CAS  PubMed  Google Scholar 

  53. L. E. Glover, J. S. Lee, and S. P. Colgan, J. Clin Invest., 126, 3680 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  54. C. J. Kim, J. Kovacs-Nolan, C. Yang, T. Archbold, M. Z. Fan, and Y. Mine, Biochim. Biophys. Acta, 1790, 1161 (2009).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, P. R. China

    Cheng Zhao, Qiongyuan Hu, Xiuwen Wu, Guopu Chen & Jianan Ren

  2. Center for New Drug Safety Evaluation and Research, China Pharmaceutical University, Nanjing, 210002, P. R. China

    Hua Wu

Authors
  1. Cheng Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiongyuan Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiuwen Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guopu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hua Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jianan Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xiuwen Wu or Jianan Ren.

Additional information

Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Acknowledgments: This study was supported by grants from Projects of Jiangsu Social Development (BE2016752, BE2017722).

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, C., Hu, Q., Wu, X. et al. Alginate and Probiotics Synergistically Reversed Dextran Sulfate Sodium Salt (DSS)-Induced Gut Barrier Damage. Macromol. Res. 27, 888–894 (2019). https://doi.org/10.1007/s13233-019-7122-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-019-7122-4

Keywords

Search

Navigation