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Development of multifunctional folate-poly(ethylene glycol)-chitosan-coated Fe3O4 nanoparticles for biomedical applications

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Abstract

The efficacy of magnetic nanoparticles (MNPs) for biomedical applications depends on the specic targeting capacity, blood circulation time and magnetic susceptibility. Functionalized chitosan-coated Fe3O4 nanoparticles (CS-coated Fe3O4 NPs) were synthesized by a non-solvent-aided coacervation procedure followed by a chemical crosslinking procedure. The surfaces of CS-coated Fe3O4 NPs were successfully functionalized with folate-poly(ethylene glycol)-COOH (FA-PEG) to obtain novel FA-PEG-CS-coated Fe3O4 NPs endowed with long blood circulation and specic targeting capacity. The as-synthesized NPs were characterized by dynamic light scattering, transmission electron microscope, X-ray diffraction, thermal gravimetric analysis, vibration sample magnetometer, Fourier transform infrared spectroscopy, and confocal laser scanning microscopy. As a result, the novel FA-PEG-CS-coated Fe3O4 NPs showed excellent biocompatibility, magnetic properties, good dispersibility, and proper hydrodynamic sizes in an aqueous medium. The specific targeting capacity of the as-synthesized NPs to cancer cells was also investigated. It was observed that the uptake of the FA-PEG-CS-coated Fe3O4 NPs by HeLa cells was significantly enhanced compared to the CS-coated Fe3O4 NPs and mPEG-CS-coated Fe3O4 NPs. These results clearly indicate that our novel FA-PEG-CS-coated Fe3O4 NPs with remarkable specific targeting capacity, long blood circulation, and superparamagnetism hold great promise for biomedical applications, including targeted drug delivery and hyperthermia therapy.

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Authors and Affiliations

  1. Research Institute for Biomimetics and Soft matter, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, P. R. China

    Shuifan Zhou, Yang Li, Fei Cui, Mengmeng Jia, Xiangrui Yang, Yange Wang & Zhenqing Hou

  2. First hospital, Xiamen University, Xiamen, 361003, P. R. China

    Liya Xie

  3. Institute of Biomedical Engineering, Chinese Academy of Medical Science &Peking Union Medical College, Tianjin, 300192, P. R. China

    Qiqing Zhang

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  1. Shuifan Zhou
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  2. Yang Li
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  3. Fei Cui
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  4. Mengmeng Jia
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  5. Xiangrui Yang
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  9. Zhenqing Hou
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Correspondence to Zhenqing Hou.

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Zhou, S., Li, Y., Cui, F. et al. Development of multifunctional folate-poly(ethylene glycol)-chitosan-coated Fe3O4 nanoparticles for biomedical applications. Macromol. Res. 22, 58–66 (2014). https://doi.org/10.1007/s13233-014-2008-y

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  • DOI: https://doi.org/10.1007/s13233-014-2008-y

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