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Controlled release of BMP-2 from alginate nanohydrogels enhanced osteogenic differentiation of human bone marrow stromal cells

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Abstract

A new controlled release system is proposed for bone morphogenetic proteins (BMP)-induced osteoblastic growth and differentiation of the human bone marrow stromal cells (hBMSCs) using injectable nanohydrogels. Alginate nanohydrogels (ANH) containing BMP-2 (BMP-2@ANH) were prepared using a reverse emulsificationdiffusion technique. The mean particle diameter of the BMP-2@ANH measured by dynamic light scattering was 520 nm. The proliferation of hBMSCs was promoted with the BMP-2@ANH. The controlled release of BMP-2 from the BMP-2@ANH enhanced the differentiation of hBMSCs into osteoblasts, which was confirmed by RT-PCR and alkaline phosphate activity. With the BMP-2@ANH, calcification and phosphate deposits on the cells were confirmed by alizarin red-S staining and von-kossa staining, respectively, showing that the differentiation of hBMSCs into osteoblasts was activated by the BMP-2 released from the BMP-2@ANH. Growth factor delivery using a nanohydrogel is a potential method for facilitating the differentiation of stem cells in vivo.

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Authors and Affiliations

  1. Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu, 702-701, Korea

    Hyun Ju Lim, Han Do Ghim & Jin Hyun Choi

  2. Joint Institute for Regenerative Medicine, Kyungpook National University, Daegu, 700-721, Korea

    Hyun Ju Lim & Jin Hyun Choi

  3. Department of Plastic & Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu, 700-721, Korea

    Ho Yun Chung

  4. Joint Institute for Regenerative Medicine, Kyungpook National University, Daegu, 700-721, Korea

    Ho Yun Chung & Jeong Ok Lim

  5. Biomedical Research Institute, School of Medicine, Kyungpook National University Hospital, Daegu, 700-721, Korea

    Jeong Ok Lim

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  1. Hyun Ju Lim
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  2. Han Do Ghim
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  3. Jin Hyun Choi
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  4. Ho Yun Chung
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Correspondence to Jeong Ok Lim.

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Lim, H.J., Ghim, H.D., Choi, J.H. et al. Controlled release of BMP-2 from alginate nanohydrogels enhanced osteogenic differentiation of human bone marrow stromal cells. Macromol. Res. 18, 787–792 (2010). https://doi.org/10.1007/s13233-010-0804-6

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  • DOI: https://doi.org/10.1007/s13233-010-0804-6

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