Skip to main content
SpringerLink
Log in

Molecular characterization of basidiomycetes associated with the decayed mangrove tree Xylocarpus granatum in Thailand

  • Published:
Fungal Diversity Aims and scope Submit manuscript

Abstract

This study reports the frequency of decay (stem/butt rot) of Xylocarpus granatum trees at Hat Khanom-Mu Ko Thale Tai National Park, Nakhonsithammarat province, and other locations in Thailand, and the identification of purported causal basidiomycetes based on morphological and molecular analyses. Four survey plots at Hat Khanom-Mu Ko Thale Tai National Park were established and the incidence of butt rot determined. Percentage stem/butt rot incidence of X. granatum trees varied from 40.9 to 94.4 % with an average of 85.5 % along all four Plots. Trees in Plot 2 supported the heaviest incidence rate (94.4 %), with the lowest rate in Plot 3 (40.9 %). Ninety-two basidiomes were collected, and 46 fungal strains (50 %) isolated into axenic culture, the majority associated with tree roots (68.5 % of all collections) with 31.5 % from the tree trunks. Molecular results, based on LSU and ITS1, 2, 5.8 S rDNA analyses, confirmed that all samples belonged to the poroid genus Fulvifomes in the Hymenochaetaceae within the Hymenochaetales, Basidiomycota. The 43 specimens sequenced grouped into three clades; one clade comprised specimens isolated from only the trunks and branches (14 strains), while the remainders were from roots (29 strains) and shown to be salt tolerant. The stem/butt rot strains formed unique phylotypes which did not group with other known Fulvifomes species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Baltazar JM, Trierveiler-Pereira L, Loguercio-Leite C (2009a) A checklist of xylophilous basidiomycetes (Basidiomycota) in mangroves. Mycotaxon 107:221–224

    Article  Google Scholar 

  • Baltazar JM, Trierveiler-Pereira L, Loguercio-Leite C, Ryvarden L (2009b) Santa Catarina Island mangroves 3: a new species of Fuscoporia. Mycologia 101:859–863

    Article  PubMed  Google Scholar 

  • Banik MT, Burdsall HH Jr (1999) Incompatibility between Laetiporus cincinatus and L. sulphureus in culture. Mycotaxon 70:461–469

    Google Scholar 

  • Barnard EL (2000) Inonotus root and butt rot of pines in Florida. Plant Pathol Circ 403

  • Bunyard BA, Nicholson MS, Royse DJ (1994) A systematic assessment of Morchella using RFLP analysis of the 28 S ribosomal RNA gene. Mycologia 86:762–772

    Article  CAS  Google Scholar 

  • Campos EL, Cavalcanti MAQ (2000) Primeira ocorrência de Phellinus mangrovicus (Imaz.) Imaz. para o Brasil. Acta Bot Bras 14:263–265

    Article  Google Scholar 

  • Campos EL, Sotão HM, Cavalcanti MAQ, Luz AB (2005) Basidiomycetes de Manguezais da APA de Algodoal-Maiandeua, Pará, Brasil. Bol. Mus. Paraense Emílio Goeldi, ser Ciências Naturais 1:141–146

  • Chalermpongse A (1991) Fungal diseases in mangrove ecosystem. In: Proceeding, the 5th Silviculture Seminar in Thailand, Division of Silviculture, Royal Forest Department, Bangkok, Thailand, pp 307–338

  • Dai Y-C (2010) Hymenochaetaceae (Basidiomycota) in China. Fungal Divers 45:131–343

    Article  Google Scholar 

  • Felsentstein J (1985) Confidence intervals on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fox RTV (2001) Fungal foes in your garden. 51. Butt Rot. Mycologist 15:184–185

    Article  Google Scholar 

  • Gales K (2002) Heart rot in plantations—significance to the wood processing industry. In: Barry K (ed) Heart rots in Plantation Hardwoods in Indonesia and Australia. ACIAR Technical Report 51e. CSIRO Publishing, Canberra, pp 18–21

  • Gilbert GS, Sousa WP (2002) Host specialization among wood-decay polypore fungi in a Caribbean mangrove forest. Biotropica 34:396–404

    Google Scholar 

  • Gilbert GS, Gorospe J, Ryvarden L (2008) Host and habitat preferences of polypore fungi in Micronesian tropical flooded forests. Mycol Res 112:674–680

    Article  PubMed  Google Scholar 

  • Glaeser JA, Lindner DL (2011) Use of fungal biosystematics and molecular genetics in detection and identification of wood-decay fungi for improved forest management. Forest Pathol 41:341–348

    Article  Google Scholar 

  • Grades M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118

    Article  Google Scholar 

  • Hall T (2004) BioEdit 6.0.7. Department of Microbiology, North Carolina State University. (http://www.mbio.ncsu.edu/BioEdit/bioedit.html)

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755 (http://morphbank.ebc.uu.se/mrbayes/download.php)

    Google Scholar 

  • Jasalavich CA, Ostrofsky A, Jellison J (2000) Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA. Appl Environ Microb 66:4725–4734

    Article  CAS  Google Scholar 

  • Jones EBG, Choeyklin R (2008) Ecology of marine and freshwater basidiomycetes. In: Boddy L, Frankland JC, van West P (eds) Ecology of saprotrophic basidiomycetes. Academic, London, pp 301–324

    Chapter  Google Scholar 

  • Jones EBG, Sakayaroj J, Suetrong S, Somrithipol S, Pang KL (2009) Classification of marine Ascomycota, anamorphic taxa and Basidiomycota. Fungal Divers 35:1–203

    Google Scholar 

  • Kinge TR, Mih AM (2011) Ganoderma ryvardense sp. nov. associated with basal stem rot (BSR) disease of oil palm in Cameroon. Mycosphere 2:179–188

    Google Scholar 

  • Kishino H, Hasegawa M (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order of Hominoidea. J Mol Evol 29:170–179

    Article  PubMed  CAS  Google Scholar 

  • Larsson K-H, Parmasto E, Fischer M, Langer E, Nakasone KK, Redhead SA (2006) Hymenochaetales: a molecular phylogeny for the Hymenochaetoid clade. Mycologia 98:926–936

    Article  PubMed  Google Scholar 

  • Lee SS (2002) Overview of the heart rot problem in Acacia—gap analysis and research options. In: Barry K (ed) Heart rots in Plantation Hardwoods in Indonesia and Australia. ACIAR Technical Report 51e. CSIRO Publishing, Canberra, pp 26–34

  • Maekawa N, Suhara H, Kinjo K, Kondo R, Hoshi Y (2005) Haloaleurodiscus mangrovei gen. sp. nov. (Basidiomycota) from mangrove forests in Japan. Mycol Res 109:825–832

    Article  PubMed  Google Scholar 

  • Murrill WA (1914) Northern polypores. Privately printed, New York

  • Mwangi JG (2001) A new pest causing decline of mangrove forests in Kenya, Africa. (www.easternarc.org)

  • Núñez M, Ryvarden L (2000) East Asian Polypores Volume 1. Ganodermataceae and Hymenochaetaceae. Oslo, Norway

  • Núñez M, Ryvarden L (2001) East Asian Polypores Volume 2. Polyporaceae s. lato. Oslo, Norway

  • Primavera JH, Sadaba RS, Lebata JHL, Altamiroano JP (2004) Handbook of mangroves in the Philippines-Panay. UNESCO

  • Rahman MA (2001) Diseases and disorders of mangroves and their management. National Workshop on management of mangrove. Bangladesh Forest Research Institute, Chittagong. 15–16 May 2001

  • Rayner ADM, Boddy L (1988) Fungal decomposition of wood: its biology and ecology. Wiley, New York

    Google Scholar 

  • Rizzo DM, Gieser PT, Burdsall HH Jr (2003) Phellinus coronadensis: a new from southern Arizona, USA. Mycologia 95:74–79

    Article  PubMed  CAS  Google Scholar 

  • Ryvarden L (2000) Studies in neotropical polypores 2: a preliminary key to neotropical species of Ganoderma with a laccate pileus. Mycologia 92:180–191

    Article  Google Scholar 

  • Ryvarden L, Johansen I (1980) A preliminary polypores flora of East Africa. Oslo, Norway

  • Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (* and other methods) version 4.0b10. Sinauer, Sunderland

    Google Scholar 

  • Thompson JD, Higgin DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Tomlinson PB (1986) The botany of mangroves. Tropical biology series. Cambridge University Press, Cambridge

    Google Scholar 

  • Vollbrecht G, Johansson U, Eriksson H, Stenlid J (1995) Butt rot incidence, yield and growth pattern in a tree species experiment in southwestern Sweden. Forest Ecol Manag 87–93

  • Wagner T, Fischer M (2002) Preceedings toward a natural classification of the worldwide taxa Phellinus and Inonotus s.l., and phylogenetic relationships of allied genera. Mycologia 94:998–1016

    Article  PubMed  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JS, White TJ (eds) PCR protocol: a guide to methods and applications. Academic, San Diego, pp 315–322

    Google Scholar 

Download references

Acknowledgments

This work was supported by the TRF/BIOTEC Special Program for Biodiversity Research and Training and Petroleum Authority of Thailand (PTT) research grant BRT R_252121 and the 2011 MAB Young Scientists Award, UNESCO. We acknowledge Dr. Lily Eurwilaichitr and Dr. Janet Jennifer Luangsa-ard for their support. We are grateful to Dr. Wijarn Meepol, Ms. Apilux Loilong, Ms. Sujinda Sommai and National Park officers for field assistance. Thanks go BIOTEC Culture Collection and BIOTEC Bangkok Herbarium for providing cultures and herbarium codes. We also thank the anonymous reviewers for their valuable comments and suggestions.

Author information

Authors and Affiliations

  1. National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand

    Jariya Sakayaroj, Sita Preedanon, Satinee Suetrong, Anupong Klaysuban & E. B. Gareth Jones

  2. Kansai Research Center, Forestry and Forest Products Research Institute, Nagai-Kyutaro 68, Momoyama, Fushimi-ku, Kyoto, 612-0855, Japan

    Tsutomu Hattori

Authors
  1. Jariya Sakayaroj
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sita Preedanon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Satinee Suetrong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anupong Klaysuban
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. B. Gareth Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tsutomu Hattori
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jariya Sakayaroj.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sakayaroj, J., Preedanon, S., Suetrong, S. et al. Molecular characterization of basidiomycetes associated with the decayed mangrove tree Xylocarpus granatum in Thailand. Fungal Diversity 56, 145–156 (2012). https://doi.org/10.1007/s13225-012-0195-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13225-012-0195-4

Keywords

Search

Navigation