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Potential use of barcoding to identify aquatic hyphomycetes

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Abstract

Tetracladium is a common aquatic hyphomycete genus, whose taxonomy has been based on the morphology and development of asexual spores. Ecological surveys have relied almost exclusively on spore morphology. Since selective pressures have resulted in convergent shapes, misidentifications are a concern. To supplement morphological information, we determined COX1, ITS and D1/D2 sequences as potential barcodes on 21 strains belonging to 7 described Tetracladium species and an unidentified strain. Attempts to amplify the IGS region were unsuccessful. The ratio of intraspecific to interspecific variability was optimal with ITS, which also provided the intuitively most acceptable cladogram. Typical conidia and their variability for the seven described species are illustrated. Internal node reliability depended less on total sequence length and more on the mixture of conserved and variable regions used to build cladograms. This finding can be exploited for quickly increasing phylogenetic accuracy without greatly increasing the amount of amplification and sequencing. The results have important implications for identifying freshwater hyphomycetes in the field but further work is required to establish if this method works with plant pathogens.

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Acknowledgements

This work was financed by a Discovery grant from the Natural Science and Engineering Research Council of Canada to FB. It is a part of the project MSM00216222416 (LM).

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Authors and Affiliations

  1. Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada, K1S 5B6

    A. Letourneau

  2. CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    S. Seena

  3. Czech Collection of Microorganisms, Institute of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic

    L. Marvanová

  4. Department of Biology, Mt. Allison University, Sackville, NB, E4L1G7, Canada

    F. Bärlocher

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  1. A. Letourneau
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  2. S. Seena
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  3. L. Marvanová
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  4. F. Bärlocher
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Correspondence to F. Bärlocher.

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Letourneau, A., Seena, S., Marvanová, L. et al. Potential use of barcoding to identify aquatic hyphomycetes. Fungal Diversity 40, 51–64 (2010). https://doi.org/10.1007/s13225-009-0006-8

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