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Unraveling the cellulolytic and hemicellulolytic potential of two novel Streptomyces strains

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Abstract

The Streptomyces spp. are notorious plant biomass decomposers in soil environments, but only few strains were biochemically and genetically characterized. Here, we employed functional screening along with genomic sequencing for identification of novel lignocellulolytic Streptomyces strains. Streptomyces strains isolated from soil were functional screened based on their cellulolytic and hemicellulolytic capacities by enzymatic plate assays containing carboxymethylcellulose (CMC) and beechwood xylan as sole carbon source. Subsequently, genomes of Streptomyces strains were sequenced, annotated, and interpreted to correlate their genetic contents with biochemical properties. Among the 80 bacterial isolates that were screened for enzymatic activity, two Streptomyces strains (named as F1 and F7) exhiting higher endoglucanase and endoxylanase activities were selected for biochemical and genomic characterization. After cultivation on steam-pretreated sugarcane bagasse-based medium, the supernatant of the strains F1 and F7 exhibited enzymatic activity against different substrates, such as arabinan, rye arabinoxylan, β-glucan, starch, CMC, xylan, and chitin. Furthermore, strain F7 was able to degrade pectin, mannan, and lichenan. The genomic analysis of both strains revealed a diversity of carbohydrate-active enzymes. The F1 and F7 genomes encode 33 and 44 different types of glycosyl hydrolases families, respectively. Moreover, the genomic analysis also identified genes related to degradation of lignin-derived aromatic compounds. Collectively, the study revealed two novel Streptomyces strains and further insights on the degradation capability of lignocellulolytic bacteria, from which a number of technologies can arise, such as saccharification processes.

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Funding

This work was financially supported by grants from National Counsel of Technological and Scientific Development—CNPq (442333/2014-5 and 310186/2014-5) and São Paulo Research Foundation—FAPESP (14/50371-8 and 15/50590-4). R.R.M and G.T. were supported by FAPESP fellowship (2017/14253-9; 2015/23279-6, respectively). We thank the Brazilian Bioethanol Science and Technology Laboratory CTBE /NGS Sequencing Facility. 

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  1. Ricardo Rodrigues de Melo and Geizecler Tomazetto contributed equally to this work.

Authors and Affiliations

  1. Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, SP, Brazil

    Ricardo Rodrigues de Melo & Gabriela Felix Persinoti

  2. Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil

    Ricardo Rodrigues de Melo & Hélia Harumi Sato

  3. Programa de Processos Tecnológicos e Ambientais, Universidade de Sorocaba (UNISO), Sorocaba, SP, Brazil

    Geizecler Tomazetto & Fabio Marcio Squina

  4. Instituto de Biociências, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil

    Roberto Ruller

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  1. Ricardo Rodrigues de Melo
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  2. Geizecler Tomazetto
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  3. Gabriela Felix Persinoti
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Correspondence to Fabio Marcio Squina.

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de Melo, R.R., Tomazetto, G., Persinoti, G.F. et al. Unraveling the cellulolytic and hemicellulolytic potential of two novel Streptomyces strains. Ann Microbiol 68, 677–688 (2018). https://doi.org/10.1007/s13213-018-1374-7

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