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Characterization of activity and microbial diversity of typical types of Daqu for traditional Chinese vinegar

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Abstract

Daqu is a fermentation starter used in traditional Chinese vinegar production and determines the quality of the final vinegar products. In this study, the activity and microbial diversity of three typical types of Daqu were investigated using a nested PCR–denaturing gradient gel electrophoresis (DGGE) and plating technique. The bacterial DGGE profile and plate analysis indicated that the bacterial composition of Daqu was dominated by Bacillus sp., Pantoea sp., Saccharopolyspora sp., Enterobacter cloaceae and Cronobacter sakazakii, and lactic acid bacteria including Weissella sp., Pediococcus pentosaceus. DGGE analysis of fungal plates identified Aspergillus niger, Absidia corymbifera, Rhizopus sp. and Penicillium sp. as the predominant fungi involved in Daqu. However, DGGE results indicated that the bacterial microbiota of QC (Qing-Cha) and HX (Hong-Xin) were more complex than that of HR (Huai-Rang) Daqu. The total populations of microorganisms in QC were about 1 log CFU g−1 higher than in HR and HX Daqu. Moreover, the saccharifying power of QC was significantly higher than in HR and HX Daqu, and the liquefying power of QC was significantly higher than in HR, but slightly lower than in HX Daqu. Our results indicate that microbial diversity plays a crucial role in determining the activity of typical types of Daqu.

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References

  • Bae S, Fleet G, Heard G (2006) Lactic acid bacteria associated with wine grapes from several Australian vineyards. J Appl Microbiol 100:712–727

    Article  CAS  PubMed  Google Scholar 

  • Chen T, Wang M, Jiang S, Xiong S, Zhu D, Wei H (2011) Investigation of the microbial changes during koji-making process of Douchi by culture-dependent techniques and PCR–DGGE. Int J Food Sci Technol 46:1878–1883

    Article  CAS  Google Scholar 

  • Cocolin L, Manzano M, Cantoni C, Comi G (2001) Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages. Appl Environ Microb 67:5113–5121

    Article  CAS  Google Scholar 

  • Duchaine C, Meriauc A, Brochu G, Bernard K, Cormier Y (1999) Saccharopolyspora rectivirgula from Quebec dairy barns: application of simplified criteria for the identification of an agent responsible for farmer's lung disease. J Med Microbiol 48:173–180

    Article  CAS  PubMed  Google Scholar 

  • Haruta S, Ueno S, Egawa I, Hashiguchi K, Fujii A, Nagano M, Ishii M, Igarashi Y (2006) Succession of bacterial and fungal communities during a traditional pot fermentation of rice vinegar assessed by PCR–mediated denaturing gradient gel electrophoresis. Int J Food Microbiol 109:79–87

    Article  CAS  PubMed  Google Scholar 

  • Hesseltine C, Rogers R, Winarno F (1988) Microbiological studies on amylolytic oriental fermentation starters. Mycopathologia 101:141–155

    Article  Google Scholar 

  • Jeon E, Sung Eun L, Park B-S, Kim S-W, Lee J, Han SO (2009) Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera β-glucosidase. FEMS Microbiol Lett 301:130–136

    Article  CAS  PubMed  Google Scholar 

  • Jeyaram K, Singh WM, Capece A, Romano P (2008) Molecular identification of yeast species associated with ‘Hamei’—a traditional starter used for rice wine production in Manipur, India. Int J Food Microbiol 124:115–125

    Article  CAS  PubMed  Google Scholar 

  • Kim T-W, Lee J-H, Park M-H, Kim H-Y (2010) Analysis of bacterial and fungal communities in Japanese- and Chinese- fermented soybean pastes using nested PCR–DGGE. Curr Microbiol 60:315–320

    Article  CAS  PubMed  Google Scholar 

  • Kim Y-S, Kim M-C, Kwon S-W, Kim S-J, Park I-C, Ka J-O, Weon H-Y (2011) Analyses of bacterial communities in meju, a Korean traditional fermented soybean bricks, by cultivation-based and pyrosequencing methods. J Microbiol 49:340–348

    Article  PubMed  Google Scholar 

  • Lane D (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, pp 115–175

    Google Scholar 

  • Li H, Lian B, Ding Y, Nie C, Zhang Q (2014a) Bacterial diversity in the central black component of Maotai Daqu and its flavor analysis. Ann Microbiol 64:1659–1669

    Article  CAS  Google Scholar 

  • Li P, Li S, Cheng L, Luo L (2014b) Analyzing the relation between the microbial diversity of DaQu and the turbidity spoilage of traditional Chinese vinegar. Appl Microbiol Biotechnol 98:6773–6084

    Google Scholar 

  • Lv X-C, Huang R-L, Chen F, Zhang W, Rao P-F, Ni L (2013) Bacterial community dynamics during the traditional brewing of Wuyi Hong Qu glutinous rice wine as determined by culture-independent methods. Food Control 34:300–306

    Article  CAS  Google Scholar 

  • Ministry of Light Industry of China (1993) General methods of determination for industrial enzymes (QB/T 1803–1993). China Light Industry Press, Beijing

    Google Scholar 

  • Nout M (2009) Rich nutrition from the poorest—cereal fermentations in Africa and Asia. Food Microbiol 26:685–692

    Article  PubMed  Google Scholar 

  • Oda K, Kakizono D, Yamada O, Iefuji H, Akita O, Iwashita K (2006) Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions. Appl Microbiol Biotechnol 72:3448–3457

    CAS  Google Scholar 

  • Porcellato D, Grønnevik H, Rudi K, Narvhus J, Skeie S (2012) Rapid lactic acid bacteria identification in dairy products by high-resolution melt analysis of DGGE bands. Lett Appl Microbiol 54:344–351

    Article  CAS  PubMed  Google Scholar 

  • Prakitchaiwattana CJ, Fleet GH, Heard GM (2004) Application and evaluation of denaturing gradient gel electrophoresis to analyse the yeast ecology of wine grapes. FEMS Yeast Res 4:865–877

    Article  CAS  PubMed  Google Scholar 

  • Sinkuniene D, Kiriliauskaite V, Bendikiene V, Juodka B (2008) Properties and immobilization of Enterobacter aerogenes 13 Lipase. Roum Biotechnol Lett 13:1–8

    CAS  Google Scholar 

  • Sujaya I, Antara N, Sone T, Tamura Y, Aryanta W, Yokota A, Asano K, Tomita F (2004) Identification and characterization of yeasts in brem, a traditional Balinese rice wine. World J Microbiol Biotechnol 20:143–150

    Article  CAS  Google Scholar 

  • Sunesen L, Stahnke L (2003) Mould starter cultures for dry sausages—selection, application and effects. Meat Sci 65:935–948

    Article  CAS  PubMed  Google Scholar 

  • Thanh VN, Mai LT, Tuan DA (2008) Microbial diversity of traditional Vietnamese alcohol fermentation starters (banh men) as determined by PCR-mediated DGGE. Int J Food Microbiol 128:268–273

    Article  CAS  PubMed  Google Scholar 

  • Tzanetakis N, Litopoulou-Tzanetaki E (1989) Biochemical activities of Pediococcus pentosaceus isolates of dairy origin. J Dairy Sci 72:859–863

    Article  CAS  Google Scholar 

  • Vainio EJ, Hantula J (2000) Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA. Mycol Res 104:927–936

    Article  CAS  Google Scholar 

  • Wah TT, Walaisri S, Assavanig A, Niamsiri N, Lertsiri S (2013) Co-culturing of Pichia guilliermondii enhanced volatile flavor compound formation by Zygosaccharomyces rouxii in the model system of Thai soy sauce fermentation. Int J Food Microbiol 160:282–289

    Article  CAS  PubMed  Google Scholar 

  • Wang HY, Gao YB, Fan QW, Xu Y (2011) Characterization and comparison of microbial community of different typical Chinese liquor Daqus by PCR–DGGE. Lett Appl Microbiol 53:134–140

    Article  CAS  PubMed  Google Scholar 

  • Xiu L, Kunliang G, Hongxun Z (2012) Determination of microbial diversity in Daqu, a fermentation starter culture of Maotai liquor, using nested PCR-denaturing gradient gel electrophoresis. World J Microbiol Biotechnol 28:2375–2381

  • Xu W, Huang Z, Zhang X, Li Q, Lu Z, Shi J, Xu Z, Ma Y (2011) Monitoring the microbial community during solid-state acetic acid fermentation of Zhenjiang aromatic vinegar. Food Microbiol 28:1175–1181

    Article  CAS  PubMed  Google Scholar 

  • Yoshikawa S, Yasokawa D, Nagashima K, Yamazaki K, Kurihara H, Ohta T, Kawai Y (2010) Microbiota during fermentation of chum salmon (Oncorhynchus keta) sauce mash inoculated with halotolerant microbial starters: analyses using the plate count method and PCR-denaturing gradient gel electrophoresis (DGGE). Food Microbiol 27:509–514

    Article  CAS  PubMed  Google Scholar 

  • Yousif NM, Huch M, Schuster T, Cho G-S, Dirar HA, Holzapfel WH, Franz CM (2010) Diversity of lactic acid bacteria from Hussuwa, a traditional African fermented sorghum food. Food Microbiol 27:757–768

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Zhao J, Du X (2014) Barcoded pyrosequencing analysis of the bacterial community of Daqu for light-flavour Chinese liquor. Lett Appl Microbiol 58:549–555

    Article  CAS  PubMed  Google Scholar 

  • Zheng XW, Tabrizi MR, Nout M, Han BZ (2011) Daqu—a traditional Chinese liquor fermentation starter. J Inst Brewing 117:82–90

    Article  CAS  Google Scholar 

  • Zheng XW, Yan Z, Han BZ, Zwietering MH, Samson RA, Boekhout T, Robert Nout M (2012) Complex microbiota of a Chinese “Fen” liquor fermentation starter (Fen Daqu), revealed by culture-dependent and culture-independent methods. Food Microbiol 31:293–300

    Article  CAS  PubMed  Google Scholar 

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Acknowledgment

This project was supported by the National Natural Foundation of China (grant 31271924).

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China

    Pan Li, Francis Worlanyo Kwami Aflakpui, Hang Yu, Lixin Luo & Wei-Tie Lin

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  1. Pan Li
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  2. Francis Worlanyo Kwami Aflakpui
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  3. Hang Yu
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  4. Lixin Luo
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  5. Wei-Tie Lin
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Correspondence to Lixin Luo or Wei-Tie Lin.

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Li, P., Aflakpui, F.W.K., Yu, H. et al. Characterization of activity and microbial diversity of typical types of Daqu for traditional Chinese vinegar. Ann Microbiol 65, 2019–2027 (2015). https://doi.org/10.1007/s13213-015-1040-2

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  • DOI: https://doi.org/10.1007/s13213-015-1040-2

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