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Activity of crude and fractionated extracts by lactic acid bacteria (LAB) isolated from local dairy, meat, and fermented products against Staphylococcus aureus

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Abstract

This study aimed to evaluate anti-staphylococcal properties of crude and fractionated extracts of lactic acid bacteria (LAB) isolated from local meat, dairy, and fermented products. A total of 36 LAB isolates were obtained and identified via 16S rDNA sequencing. Cell-free supernatant (CFS) of all isolates exhibiting a statistically significant inhibition against Staphylococcus aureus (ρ < 0.05), with six LAB isolates exhibiting a more prevalent inhibition. The inhibition effects of cell wall and intracellular extracts from the six prevalent isolates were evaluated. Lactobacillus plantarum USM8613 was the most prominent isolate with both CFS and cell wall extract exhibiting the most prevalent inhibition against S. aureus. Scanning electron micrographs showed alteration of S. aureus membrane morphology upon CFS treatment, suggesting an anti-staphylococcal effect via membrane destruction. Confocal laser scanning micrographs showed inhibition against biofilm formations by S. aureus in porcine skins upon CFS treatment. The CFS from L. plantarum USM8613 was separated into protein, lipid, and polysaccharide fractions for evaluation of anti-staphylococcal activity and chemical characterization. All fractions inhibited growth of S. aureus (ρ < 0.05), with protein fractions exhibiting stronger inhibition effect. Data from our present study showed that extracts from LAB could be applied as biopreservatives in the food industries and/or as an antimicrobial agent against bacterial infections for cosmeceutical and pharmaceutical uses.

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Acknowledgments

This work was financially supported by the Science Fund Grant (305/PTEKIND/613222) provided by the Malaysian Ministry of Science, Technology, and Innovation (MOSTI), the FRGS grant (203/PTEKIND/6711239) provided by the Malaysian Ministry of Higher Education (MOHE), USM RU grants (1001/PKIMIA/855006, 1001/PTEKIND/815085), and USM Fellowship provided by Universiti Sains Malaysia.

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Authors and Affiliations

  1. Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Cheng-Chung Yong, Li-Oon Chuah & Min-Tze Liong

  2. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Boon-Yin Khoo & Sreenivasan Sasidharan

  3. National Electronics and Computer Technology Center, Pathumthani, Thailand

    Wibool Piyawattanametha & Numfon Khemthongcharoen

  4. Advanced Imaging Research Center, Faculty of Medicine, Chulalongkorn University, Pathumwan, Thailand

    Wibool Piyawattanametha & Numfon Khemthongcharoen

  5. Division of Food Bioscience and Technology, Korea University, Seoul, 136-701, South Korea

    Sae-Hun Kim

  6. Fisher Scientific (M) Sdn Bhd, No. 3, Jalan Sepadu 25/123, Taman Perindustrian Axis, Seksyen 25, 40400, Shah Alam, Selangor Darul Ehsan, Malaysia

    May-Yen Ang

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  1. Cheng-Chung Yong
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Correspondence to Min-Tze Liong.

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Yong, CC., Khoo, BY., Sasidharan, S. et al. Activity of crude and fractionated extracts by lactic acid bacteria (LAB) isolated from local dairy, meat, and fermented products against Staphylococcus aureus . Ann Microbiol 65, 1037–1047 (2015). https://doi.org/10.1007/s13213-014-0949-1

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