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Decolorization and biodegradation of triphenylmethane dyes by a novel Rhodococcus qingshengii JB301 isolated from sawdust

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Abstract

A sawdust sample collected from a wet environment was used to screen for crystal violet-decolorizing and methyl violet-decolorizing bacterial strains. A strain with degrading and decolorizing abilities was isolated and identified as Rhodococcus qingshengii JB301. The decolorization of crystal violet and methyl violet was improved by optimizing culture conditions. Based on liquid chromatography–mass spectrometry (LC-MS) analysis, similar degradation products of crystal violet and methyl violet were detected, while [N,N-dimethylaminophenyl] [aminophenyl] benzophenone or [N-methylaminophenyl] [N-methylaminophenyl] benzophenone was different from previous reports. A common degradation pathway was established to demonstrate the mechanism of crystal violet and methyl violet decolorization. It was found that lignin peroxidase activity was greatly enhanced after addition of crystal violet (by 514 %) and methyl violet (by 698 %) at 12 h, while NADH-DCIP (dichlorophenolindophenol) reductase activity was inhibited by the dyes. The growth of Triticum aestivum was inhibited by both dyes and the toxicity of the dyes was weakened after the degradation by R. qingshengii JB301.

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References

  • An SY, Min SK, Cha IH, Choi YL, Cho YS, Kim CH, Lee YC (2002) Decolorization of triphenylmethane and azo dyes by Citrobacter sp. Biotechnol Lett 24:1037–1040

    Article  CAS  Google Scholar 

  • Ayed L, Cheriaa J, Laadhari N, Cheref A, Bakhrouf A (2009) Biodegradation of crystal violet by an isolated Bacillus sp. Ann Microbiol 59:267–272

    Article  CAS  Google Scholar 

  • Behki R, Topp E, Dick W, Germon P (1993) Metabolism of the herbicide atrazine by Rhodococcus strains. Appl Environ Microbiol 59:1955–1959

    CAS  PubMed Central  PubMed  Google Scholar 

  • Bell KS, Philp JC, Aw DWJ, Christofi N (1998) The genus Rhodococcus. J Appl Microbiol 85:195–210

    Article  CAS  PubMed  Google Scholar 

  • Beveridge TJ (2001) Use of the Gram stain in microbiology. Biotech Histochem 76:111–118

    Article  CAS  PubMed  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Briglia M, Rainey FA, Stackebrandt E, Schraa G, Salkinoja-Salonen MS (1996) Rhodococcus percolatus sp. nov., a bacterium degrading 2,4,6-trichlorophenol. Int J Syst Evol Microbiol 46:23–30

    CAS  Google Scholar 

  • Chander M, Arora DS (2007) Evaluation of some white-rot fungi for their potential to decolourise industrial dyes. Dyes Pigments 72:192–198

    Article  CAS  Google Scholar 

  • Chang WN, Liu CW, Liu HS (2009) Hydrophobic cell surface and bioflocculation behavior of Rhodococcus erythropolis. Process Biochem 44:955–962

    Article  CAS  Google Scholar 

  • Chen KC, Wu JY, Liou DJ, Hwang SCJ (2003) Decolorization of the textile dyes by newly isolated bacterial strains. J Biotechnol 101:57–68

    Article  CAS  PubMed  Google Scholar 

  • Chen CH, Chang CF, Ho CH, Tsai TL, Liu SM (2008) Biodegradation of crystal violet by a Shewanella sp. NTOU1. Chemosphere 72:1712–1720

    Article  CAS  PubMed  Google Scholar 

  • Chen CH, Chang CF, Liu SM (2010) Partial degradation mechanisms of malachite green and methyl violet B by Shewanella decolorationis NTOU1 under anaerobic conditions. J Hazard Mater 177:281–289

    Article  CAS  PubMed  Google Scholar 

  • Chen CC, Chen CY, Cheng CY, Teng PY, Chung YC (2011) Decolorization characteristics and mechanism of Victoria Blue R removal by Acinetobacter calcoaceticus YC210. J Hazard Mater 196:166–172

    Article  CAS  PubMed  Google Scholar 

  • Culp SJ, Mellick PW, Trotter RW, Greenlees KJ, Kodell RL, Beland FA (2006) Carcinogenicity of malachite green chloride and leucomalachite green in B6C3F(1) mice and F344 rats. Food Chem Toxicol 44:1204–1212

    Article  CAS  PubMed  Google Scholar 

  • Dantan-Gonzalez E, Vite-Vallejo O, Martinez-Anaya C, Mendez-Sanchez M, Gonzalez MC, Palomares LA, Folch-Mallol J (2008) Production of two novel laccase isoforms by a thermotolerant strain of Pycnoporus sanguineus isolated from an oil-polluted tropical habitat. Int Microbiol 11:163–169

    CAS  PubMed  Google Scholar 

  • de las Heras LF, Mascaraque V, García Fernández EG, Navarro-Llorens JM, Perera J, Drzyzga O (2011) ChoG is the main inducible extracellular cholesterol oxidase of Rhodococcus sp. strain CECT3014. Microbiol Res 166:403–418

    Article  Google Scholar 

  • Dean-Ross D, Moody JD, Freeman JP, Doerge DR, Cerniglia CE (2001) Metabolism of anthracene by a Rhodococcus species. FEMS Microbiol Lett 204:205–211

    Article  CAS  PubMed  Google Scholar 

  • Fessard V, Godard T, Huet S, Mourot A, Poul JM (1999) Mutagenicity of malachite green and leucomalachite green in in vitro tests. J Appl Toxicol 19:421–430

    Article  CAS  PubMed  Google Scholar 

  • Franciscon E, Piubeli F, Fantinatti-Garboggini F, de Menezes CR, Silva IS, Cavaco-Paulo A, Grossman MJ, Durrant LR (2010) Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme. Enzym Microb Technol 46:360–365

    Article  CAS  Google Scholar 

  • Hammel KE, Cullen D (2008) Role of fungal peroxidases in biological ligninolysis. Curr Opin Plant Biol 11:349–355

    Article  CAS  PubMed  Google Scholar 

  • Hilden K, Hakala TK, Lundell T (2009) Thermotolerant and thermostable laccases. Biotechnol Lett 31:1117–1128

    Article  CAS  PubMed  Google Scholar 

  • Jadhav JP, Govindwar SP (2006) Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463. Yeast 23:315–323

    Article  CAS  PubMed  Google Scholar 

  • Jadhav UU, Dawkar VV, Ghodake GS, Govindwar SP (2008) Biodegradation of Direct Red 5B, a textile dye by newly isolated Comamonas sp. UVS. J Hazard Mater 158:507–516

    Article  CAS  PubMed  Google Scholar 

  • Kalme SD, Parshetti GK, Jadhav SU, Govindwar SP (2007) Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112. Bioresour Technol 98:1405–1410

    Article  CAS  PubMed  Google Scholar 

  • Kalyani DC, Patil PS, Jadhav JP, Govindwar SP (2008) Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresour Technol 99:4635–4641

    Article  CAS  PubMed  Google Scholar 

  • Kamble AL, Meena VS, Banerjee UC (2010) Effect of agitation and aeration on the production of nitrile hydratase by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor. Lett Appl Microbiol 51:413–420

    Article  CAS  PubMed  Google Scholar 

  • Khataee AR, Pons MN, Zahraa O (2009) Photocatalytic degradation of three azo dyes using immobilized TiO(2) nanoparticles on glass plates activated by UV light irradiation: influence of dye molecular structure. J Hazard Mater 168:451–457

    Article  CAS  PubMed  Google Scholar 

  • Khataee AR, Dehghan G, Zarei M, Ebadi E, Pourhassan M (2011) Neural network modeling of biotreatment of triphenylmethane dye solution by a green macroalgae. Chem Eng Res Des 89:172–178

    Article  CAS  Google Scholar 

  • Kumar KV, Sivanesan S, Ramamurthi V (2005) Adsorption of malachite green onto Pithophora sp., a fresh water algae: Equilibrium and kinetic modelling. Process Biochem 40:2865–2872

    Article  CAS  Google Scholar 

  • Kumar CG, Mongolla P, Basha A, Joseph J, Sarma VUM, Kamal A (2011) Decolorization and Biotransformation of Triphenylmethane Dye, Methyl Violet, by Aspergillus sp. Isolated from Ladakh, India. J Microbiol Biotechnol 21:267–273

    CAS  PubMed  Google Scholar 

  • Liu RJ, Jin QZ, Tao GJ, Shan L, Liu YF, Wang XG (2010) LC-MS and UPLC-quadrupole time-of-flight MS for identification of photodegradation products of aflatoxin B(1). Chromatographia 71:107–112

    Article  CAS  Google Scholar 

  • Maalej-Kammoun M, Zouari-Mechichi H, Belbahri L, Woodward S, Mechichi T (2009) Malachite green decolourization and detoxification by the laccase from a newly isolated strain of Trametes sp. Int Biodeterior Biodegrad 63:600–606

    Article  CAS  Google Scholar 

  • Maki M, Leung KT, Qin WS (2009) The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass. Int J Biol Sci 5:500–516

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Manal MA, El-Naggar S, El-Aasar A, Khlood IB (2005) Bioremediation of crystal violet using air bubble bioreactor packed with Pseudomonas aeruginosa. Water Res 39:5045–5054

    Article  CAS  PubMed  Google Scholar 

  • Marques AM, Pinazo A, Farfan M, Aranda FJ, Teruel JA, Ortiz A, Manresa A, Espuny MJ (2009) The physicochemical properties and chemical composition of trehalose lipids produced by Rhodococcus erythropolis 51T7. Chem Phys Lipids 158:110–117

    Article  CAS  PubMed  Google Scholar 

  • Martínez AT (2002) Molecular biology and structure-function of lignin-degrading heme peroxidases. Enzym Microb Technol 30:425–444

    Article  Google Scholar 

  • Mittal A, Kurup L, Gupta VK (2005) Use of waste materials - Bottom Ash and De-Oiled Soya, as potential adsorbents for the removal of Amaranth from aqueous solutions. J Hazard Mater 117:171–178

    Article  CAS  PubMed  Google Scholar 

  • Moosvi S, Keharia H, Madamwar D (2005) Decolourization of textile dye Reactive Violet 5 by a newly isolated bacterial consortium RVM 11.1. World J Microbiol Biotechnol 21:667–672

    Article  CAS  Google Scholar 

  • Murugesan K, Nam IH, Kim YM, Chang YS (2007) Decolorization of reactive dyes by a thermostable laccase produced by Ganoderma lucidum in solid state culture. Enzym Microb Technol 40:1662–1672

    Article  CAS  Google Scholar 

  • Nohynek GJ, Antignac E, Re T, Toutain H (2010) Safety assessment of personal care products/cosmetics and their ingredients. Toxicol Appl Pharmacol 243:239–259

    Article  CAS  PubMed  Google Scholar 

  • Parshetti G, Kalme S, Saratale G, Govindwar S (2006) Biodegradation of malachite green by Kocuria rosea MTCC 1532. Acta Chim Slov 53:492–498

    CAS  Google Scholar 

  • Parshetti G, Saratale G, Telke A, Govindwar S (2009) Biodegradation of hazardous triphenylmethane dye methyl violet by Rhizobium radiobacter (MTCC 8161). J Basic Microbiol 49:36–42

    Article  Google Scholar 

  • Parshetti GK, Parshetti SG, Telke AA, Kalyani DC, Doong RA, Govindwar SP (2011) Biodegradation of crystal violet by Agrobacterium radiobacter. J Environ Sci-China 23:1384–1393

    Article  CAS  PubMed  Google Scholar 

  • Radha KV, Regupathi I, Arunagiri A, Murugesan T (2005) Decolorization studies of synthetic dyes using Phanerochaete chrysosporium and their kinetics. Process Biochem 40:3337–3345

    Article  CAS  Google Scholar 

  • Raghukumar C, Chandramohan D, Michel FC, Redd CA (1996) Degradation of lignin and decolorization of paper mill bleach plant effluent (BPE) by marine fungi. Biotechnol Lett 18:105–106

    Article  CAS  Google Scholar 

  • Ren SZ, Guo J, Zeng GQ, Sun GP (2006) Decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain. Appl Microbiol Biotechnol 72:1316–1321

    Article  CAS  PubMed  Google Scholar 

  • Robinson T, McMullan G, Marchant R, Nigam P (2001) Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresour Technol 77:247–255

    Article  CAS  PubMed  Google Scholar 

  • Salokhe MD, Govindwar SP (1999) Effect of carbon source on the biotransformation enzymes in Serratia marcescens. World J Microbiol Biotechnol 15:259–263

    Article  Google Scholar 

  • Sani RK, Banerjee UC (1999) Decolorization of triphenylmethane dyes and textile and dye-stuff effluent by Kurthia sp. Enzym Microb Technol 24:433–437

    Article  CAS  Google Scholar 

  • Saratale RG, Saratale GD, Chang JS, Govindwar SP (2009) Decolorization and biodegradation of textile dye Navy blue HER by Trichosporon beigelii NCIM-3326. J Hazard Mater 166:1421–1428

    Article  CAS  PubMed  Google Scholar 

  • Selvam K, Swaminathan K, Chae KS (2003) Decolourization of azo dyes and a dye industry effluent by a white rot fungus Thelephora sp. Bioresour Technol 88:115–119

    Article  CAS  PubMed  Google Scholar 

  • Shedbalkar U, Dhanve R, Jadhav J (2008) Biodegradation of triphenylmethane dye cotton blue by Penicillium ochrochloron MTCC 517. J Hazard Mater 157:472–479

    Article  CAS  PubMed  Google Scholar 

  • Soloman PA, Basha CA, Velan M, Balasubramanian N (2010) Electro oxidation of malachite green and modeling using ANN. Chem Biochem Eng Q 24:445–452

    CAS  Google Scholar 

  • Tien M, Kirk TK (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proc Natl Acad Sci U S A 81:2280–2284

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Biotechnol 173:697–703

    CAS  Google Scholar 

  • Xu JL, He J, Wang ZC, Wang K, Li WJ, Tang SK, Li SP (2007) Rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium. Int J Syst Evol Microbiol 57:2754–2757

    Article  CAS  PubMed  Google Scholar 

  • Yang XQ, Wang JR, Zhao XX, Wang Q, Xue R (2011a) Increasing manganese peroxidase production and biodecolorization of triphenylmethane dyes by novel fungal consortium. Bioresour Technol 102:10535–10541

    Article  CAS  PubMed  Google Scholar 

  • Yang YY, Hu H, Wang G, Li ZL, Wang B, Jia XM, Zhao YH (2011b) Removal of malachite green from aqueous solution by immobilized Pseudomonas sp. DY1 with Aspergillus oryzae. Int Biodeterior Biodegrad 65:429–434

    Article  CAS  Google Scholar 

  • Yatome C, Ogawa T, Matsui M (1991) Degradation of crystal violet by Bacillus subtilis. J Environ Sci Health A 26:75–87

    Google Scholar 

  • Yatome C, Yamada S, Ogawa T, Matsui M (1993) Degradation of crystal violet by Nocardia corallina. Appl Microbiol Biotechnol 38:565–569

    CAS  Google Scholar 

  • Yoon JH, Cho YG, Kang SS, Kim SB, Lee ST, Park YH (2000a) Rhodococcus koreensis sp. nov., a 2,4-dinitrophenol-degrading bacterium. Int J Syst Evol Microbiol 50:1193–1201

    Article  CAS  Google Scholar 

  • Yoon JH, Kang SS, Cho YG, Lee ST, Kho YH, Kim CJ, Park YH (2000b) Rhodococcus pyridinivorans sp. nov., a pyridine-degrading bacterium. Int J Syst Evol Microbiol 50:2173–2180

    Article  CAS  Google Scholar 

  • Zheng TL, Wang H, Su JQ, Zheng XW, Tian Y, Xiong XJ (2009) Bacterial decolorization and degradation of the reactive dye Reactive Red 180 by Citrobacter sp CK3. Int Biodeterior Biodegrad 63:395–399

    Article  Google Scholar 

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Acknowledgements

This research was supported by a grant from the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People’s Republic of China

    Guohui Li, Lin Peng, Zhongyang Ding, Liang Zhang & Guiyang Shi

  2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Guohui Li, Lin Peng, Zhongyang Ding, Zhenghua Gu, Liang Zhang & Guiyang Shi

  3. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Yuanfa Liu

  4. Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People’s Republic of China

    Guohui Li

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  1. Guohui Li
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  2. Lin Peng
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  3. Zhongyang Ding
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Correspondence to Zhongyang Ding.

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Li, G., Peng, L., Ding, Z. et al. Decolorization and biodegradation of triphenylmethane dyes by a novel Rhodococcus qingshengii JB301 isolated from sawdust. Ann Microbiol 64, 1575–1586 (2014). https://doi.org/10.1007/s13213-014-0801-7

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