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Production of ganoderic acid by Ganoderma lucidum RCKB-2010 and its therapeutic potential

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Abstract

The newly isolated basidiomycetous fungus, identified as Ganoderma lucidum RCKB-2010 was tested for production of ganoderic acid (GA) under submerged fermentation conditions. Production of GA under liquid static cultivation condition was found to be 2,755.88 mg L−1 on the 25th day of incubation, whereas under shaking cultivation conditions the maximum production of GA was observed to be 373.75 mg L−1. 1H NMR analysis revealed clearly that the fungal extracts possessed a lanostane skeleton, confirming the presence of GA. Interestingly, GA was found to have potential to inhibit the proliferation of HeLa cells and U87 human glioma cells in a dose dependent manner. In addition, GA was also found to possess antibacterial activity, exhibiting a minimal inhibitory concentration of 0.25 mg mL−1 against standard strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis. GA produced in the present study holds potential as a potent anticancer agent.

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Acknowledgement

Financial support from University of Delhi South Campus, New Delhi, India for accomplishment of this piece of work is highly acknowledged.

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Authors and Affiliations

  1. Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Biotech Park Building, Ground Floor, New Delhi, 110021, India

    Mohita Upadhyay, Bhuvnesh Shrivastava & Ramesh Chander Kuhad

  2. Green Research Laboratory, Department of Chemistry, University of Delhi, Delhi, 110007, India

    Arti Jain & Mazaahir Kidwai

  3. School of Biological Sciences, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

    Sanjay Kumar & James Gomes

  4. Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi, India, 110067

    Dinesh G. Goswami & Amulya Kumar Panda

Authors
  1. Mohita Upadhyay
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  2. Bhuvnesh Shrivastava
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  3. Arti Jain
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  4. Mazaahir Kidwai
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  5. Sanjay Kumar
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  6. James Gomes
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  7. Dinesh G. Goswami
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  8. Amulya Kumar Panda
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  9. Ramesh Chander Kuhad
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Corresponding author

Correspondence to Ramesh Chander Kuhad.

Additional information

Mohita Upadhyay and Bhuvnesh Shrivastava contributed equally to this study

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Upadhyay, M., Shrivastava, B., Jain, A. et al. Production of ganoderic acid by Ganoderma lucidum RCKB-2010 and its therapeutic potential. Ann Microbiol 64, 839–846 (2014). https://doi.org/10.1007/s13213-013-0723-9

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  • DOI: https://doi.org/10.1007/s13213-013-0723-9

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