Abstract
An ascomycetous yeast strain isolated from activated sludge could decolorize Reactive Black 5 azo dye at 200 mg l−1 up to 90 % within 12–18 h under agitated condition. Yeast decolorization ability was investigated at different RB5 concentrations and, at higher dye concentration, 500 mg l−1, the decolorization was found to be 98 % after 36 h incubation time. Extensive decolorization (95–99 %) was obtained in presence of five other azo dyes, Reactive Orange 16, Reactive Red 198, Direct Blue 71, Direct Yellow 12, and Direct Black 22, by isolated yeast. HPLC analysis, UV–vis spectra and colorless biomass obtained after complete decolorization showed that the decolorization occured through a biodegradation mechanism. Decolorization was occurred during the exponential growth phase which is associated to primary metabolism. Laccase production by the yeast cells was not detected. The isolated yeast was characterized according to phenotypical and molecular procedures and was closely related (99 % identity) to Issatchenkia orientalis.
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The authors would like to thank the Department of Biology of Alzahra University for their support through the study.
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Jafari, N., Soudi, M.R. & Kasra-Kermanshahi, R. Biodecolorization of textile azo dyes by isolated yeast from activated sludge: Issatchenkia orientalis JKS6. Ann Microbiol 64, 475–482 (2014). https://doi.org/10.1007/s13213-013-0677-y
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DOI: https://doi.org/10.1007/s13213-013-0677-y