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Characterisation of Arthrobacter sp. S1 that can degrade α and β-haloalkanoic acids isolated from contaminated soil

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Abstract

A bacterium identified as Arthrobacter sp. S1 by 16S rRNA was isolated from contaminated soil. This is the first reported study that Arthrobacter could utilize both α-halocarboxylix acid (αHA) [2,2-dichloropropionic acid (2,2-DCP) and D,L-2-chloropropionic acid (D,L-2-CP)] and β-halocarboxylix acid (βHA) [3-chloropropionic acid (3CP)] as sole source of carbon with cell doubling times of 5 ± 0.2, 7 ± 0.1, and 10 ± 0.1 h, respectively. More than 85 % chloride ion released was detected in the growth medium suggesting the substrates used were utilized. To identify the presence of dehalogenase gene in the microorganism, a molecular tool that included the use of oligonucleotide primers specific to microorganisms that can grow in halogenated compounds was adapted. A partial putative dehalogenase gene was determined by direct sequencing of the PCR-amplified genomic DNA of the bacterium. A comparative analysis of the deduced amino acid sequence data revealed that the amino acid sequence has a low identity of less than 15 % to both group I and group II dehalogenases, suggesting that the current putative dehalogenase amino acid sequence was completely distinct from both α-haloacids and β-haloacids dehalogenases. This investigation is useful in studying the microbial populations in order to monitor the presence of specific dehalogenase genes and to provide a better understanding of the microbial populations that are present in soil or in water systems treating halogenated compounds.

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Acknowledgments

The authors would like to express their great gratitude to the Faculty of Biosciences and Medical Engineering (FBME), Universiti Teknologi Malaysia-GUP Q.J130000.7135.00H34 and FRGS 4 F008 (MOHE-UTM) for supporting this research, and the Microbiology Research Group UNO-R, Philippines for initial isolation and characterization of the isolated microbes.

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Authors and Affiliations

  1. Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310, UTM Skudai, Malaysia

    Saeedeh Bagherbaigi, Mahdieh Nemati & Fahrul Huyop

  2. College of Arts and Sciences, University of Negros Occidental-Recoletos, 6100, Bacolod City, Philippines

    Ronnie G. Gicana & Robert J. Lamis

Authors
  1. Saeedeh Bagherbaigi
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  2. Ronnie G. Gicana
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  3. Robert J. Lamis
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  4. Mahdieh Nemati
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  5. Fahrul Huyop
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Correspondence to Fahrul Huyop.

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Bagherbaigi, S., Gicana, R.G., Lamis, R.J. et al. Characterisation of Arthrobacter sp. S1 that can degrade α and β-haloalkanoic acids isolated from contaminated soil. Ann Microbiol 63, 1363–1369 (2013). https://doi.org/10.1007/s13213-012-0595-4

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  • DOI: https://doi.org/10.1007/s13213-012-0595-4

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