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Immunomodulatory function and in vivo properties of Pediococcus pentosaceus OZF, a promising probiotic strain

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Abstract

Some of the important properties of probiotics are the ability to survive during gastrointestinal transit and to modulate the immune functions. The objectives of the reported study were to assess in vivo gastrointestinal survival of orally administered Pediococcus pentosaceus OZF using an animal model BALB/c mice, and to examine its effects on the immune response. Following oral administration to mice, the ability of Pediococcus pentosaceus OZF to pass and survive through the mouse gastrointestinal system was investigated by analyzing the recovery of the strain in fecal samples. Microbiological and polymerase chain reaction (PCR) methods proved that the strain OZF could overcome specific conditions in the gastrointestinal tract of mice and reach the intestine alive after ingestion. To observe the effect of oral administration on immune response, IL-6, IL-12 and IFN-γ were measured by ELISA, and the strain OZF was found to cause increases in IL-6 synthesis in regularly fed mice. However, stimulation was carried out with various concentrations of bacterial ssDNA and heat killed cells of Pediococcus pentosaceus OZF. The heat killed cells of the strain OZF were shown to produce IFN-γ independently from IL-12. On the other hand, a significant difference between control and experimental group was noticed when lipopolysaccharide, a TLR4 (toll like receptor) ligand, was used. Overall, Pediococcus pentosaceus OZF may be a valuable probiotic strain for therapeutic uses. Nevertheless, further studies on the mechanisms of immunomodulatory effect will allow for better clarification of the immune functions of this strain.

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Acknowledgments

This work was partially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) Project; SBAG 111S012. We thank to Tamer Kahraman and other members of Therapeutic ODN Research Laboratory for their excellent technical support.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Ankara University, Tandogan, 06100, Ankara, Turkey

    Ozlem Osmanagaoglu & Fadime Kiran

  2. Department of Molecular Biology and Genetics, Therapeutic ODN Research Laboratory, Bilkent University, Ankara, Turkey

    Fuat C. Yagci & Ihsan Gursel

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  1. Ozlem Osmanagaoglu
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  2. Fadime Kiran
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  4. Ihsan Gursel
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Correspondence to Ozlem Osmanagaoglu.

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Osmanagaoglu, O., Kiran, F., Yagci, F.C. et al. Immunomodulatory function and in vivo properties of Pediococcus pentosaceus OZF, a promising probiotic strain. Ann Microbiol 63, 1311–1318 (2013). https://doi.org/10.1007/s13213-012-0590-9

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