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Isolation of probiotic lactobacilli strains harboring l-asparaginase and arginine deiminase genes from human infant feces for their potential application in cancer prevention

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Abstract

The aim of this work was to isolate novel lactobacilli probiotic strains from human feces and screen them for the presence of two valuable antitumor genes—the arginine deiminase-encoding gene arcA and l-asparaginase-encoding gene ansA—for future potential therapeutic application in cancer prevention. Feces samples were collected from Egyptian infants. Forty-two isolates were determined as Lactobacillus sp. and selected for further characterization. Only 20 isolates exhibited good tolerance to pH 1.5, 0.3 % bile salts and moderate tolerance to pancreatic enzymes in addition to antagonistic action. These isolates were screened by PCR for the presence of the arcA and ansA genes. Three strains were selected and identified to subspecies levels by amplification and sequencing of 16S rRNA genes as Lactobacillus gasseri NM112 containing the ansA gene; Lactobacillus fermentum NM212; and Lactobacillus casei NM312 containing the arcA gene, and confirmed by determining enzyme activity. We conclude that these three strains can be suggested as probiotics with potential therapeutic effect against cancer.

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References

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25(17):3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Anukam KC, Reid G (2007) Probiotics: 100 years (1907–2007) after Elie Metchnikoff’s observations. In: Mendez-Vilas A (ed) Communicating current research and educational topics and trends in applied microbiology. http://formatex.org/, Spain, pp 466–474

  • Athale UH, Chan KCA (2003) Thrombosis in children with acute lymphoblastic leukemia. Part II. Pathogenesis of thrombosis in children with acute lymphoblastic leukemia: effects of the disease and therapy. Thromb Res 111:199–212

    Article  PubMed  CAS  Google Scholar 

  • Boyde TRC, Rahmatullah M (1980) Optimization of conditions for the colorimetric determination of citrulline, using diacetyl monoxime. Anal Biochem 107:424–431

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteinedye binding. Anal Biochem 72:248e254

    Article  Google Scholar 

  • Chen F, Wang D (2010) Novel technologies for the prevention and treatment of dental caries: a patent survey. Expert Opin Ther Pat 20:681–694

    Article  PubMed  CAS  Google Scholar 

  • Commane D, Hughes R, Shortt C, Rowland I (2005) The potential mechanisms involved in the anti-carcinogenic action of probiotics. Mutat Res 591:276–289

    Article  PubMed  CAS  Google Scholar 

  • De Angelis M, Mariotti L, Rossi J, Servili M, Fox PF, Rollán G, Gobbetti M (2002) Arginine catabolism by sourdough lactic acid bacteria: purification and characterization of the arginine deiminase pathway enzymes from Lactobacillus sanfranciscensis CB1. Appl Environ Microbiol 68:6193–6201

    Article  PubMed  Google Scholar 

  • Deshpande G, Rao S, Patole S, Bulsara M (2010) Updated meta-analysis of probiotics for preventing necrotizing enterocolitis in preterm neonates. Pediatrics 125:921–930

    Article  PubMed  Google Scholar 

  • FAO/WHO (2001) Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. http://www.who.int/entity/foodsafety/publications/fs_management/en/probiotics.pdf

  • Foster KJ, Lin S, Turck CJ (2010) Current and emerging strategies for treating hepatic encephalopathy. Crit Care Nurs Clin N Am 22:341–350

    Article  Google Scholar 

  • Galkin A, Kulakova L, Sarikaya E, Lim K, Howard A, Herzberg O (2004) Structural insight into arginine degradation by arginine deiminase, an antibacterial and parasite drug target. J Biol Chem 279:14001–14008

    Article  PubMed  CAS  Google Scholar 

  • Gilliland SE, Staley TE, Bush LJ (1984) Importance in bile tolerance of Lactobacillus acidophilus used as a diatery adjunct. J Dairy Sci 67:3045–3051

    Article  PubMed  CAS  Google Scholar 

  • Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Group 19. Regular nonsporing gram-positive rods. In: Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, Baltimore, pp 565–570

  • Horn F (1933) The breakdown of arginine to citrulline by Bacillus pyocyaneus. Hoppe Seylers Z Physiol Chem 216:244–247

    Article  CAS  Google Scholar 

  • Jacobsen CN, Rosenfeldt N, Hayford AE, Moller PL, Michaelsen KF, Paerregaard A (1999) Screening of probiotic activities of fortyseven strains of Lactobacillus spp. by in vitro techniques and evaluation of selected strains of the colonization ability of five selected strains in humans. Appl Environ Microbiol 65:4949–4956

    PubMed  CAS  Google Scholar 

  • Kafkewitz D, Goodman D (1974) l-Asparaginase production by the rumen anaerobe Vibrio succinogenes. J Appl Microbiol 27:206–209

    CAS  Google Scholar 

  • Kakimoto T, Shibatani T, Chibata I (1971) Crystallization of l-arginine deiminase from Pseudomonas putida. FEBS Lett 19:166–168

    Article  PubMed  CAS  Google Scholar 

  • Kale-Pradhan PB, Jassal HK, Wilhelm SM (2010) Role of Lactobacillus in the prevention of antibiotic-associated diarrhea: a meta-analysis. Pharmacotherapy 30:119–126

    Article  PubMed  Google Scholar 

  • Kiely LJ, Olson NF (2000) The physicochemical surface characteristics of Lactobacillus casei. Food Microbiol 17:277–291

    Article  CAS  Google Scholar 

  • Kim JE, Jeong DW, Lee HJ (2007) Expression, purification, and characterization of arginine deiminase from Lactococcus lactis ssp. lactis ATCC 7962 in Escherichia coli BL21. Protein Expr Purif 53:9–15

    Article  PubMed  CAS  Google Scholar 

  • Kumar M, Kumar A, Nagpal R, Mohania D, Behare P, Verma V, Kumar P, Poddar D, Aggarwal PK, Henry CJ, Jain S, Yadav H (2010) Cancer-preventing attributes of probiotics: an update. Int J Food Sci Nutr 61(5):473–496

    Article  PubMed  CAS  Google Scholar 

  • Lionetti E, Indrio F, Pavone L, Borrelli G, Cavallo L, Francavilla R (2010) Role of probiotics in pediatric patients with Helicobacter pylori infection: a comprehensive review of the literature. Helicobacter 15:79–87

    Article  PubMed  CAS  Google Scholar 

  • Lu X, Li L, Wu R, Feng X, Li Z, Yang H, Wang C, Guo H, Galkin A, Herzberg O, Mariano PS, Martin BM, Dunaway-Mariano D (2006) Kinetic analysis of Pseudomonas aeruginosa arginine deiminase mutants and alternate substrates provides insight into structural determinants of function. Biochemistry 45:1162–1172

    Article  PubMed  CAS  Google Scholar 

  • Mitsuoka T (1992) Intestinal flora and aging. Nutr Rev 50:438–446

    Article  PubMed  CAS  Google Scholar 

  • Narayan SS, Jalgaonkar S, Shahani S, Kulkarni VN (2010) Probiotics: current trends in the treatment of diarrhea. Hong Kong Med J 16:213–218

    PubMed  Google Scholar 

  • Narta UK, Kanwar SS, Azmi W (2007) Pharmacological and clinical evaluation of l-asparaginase in the treatment of leukemia. Crit Rev Oncol Hematol 61:208–221

    Article  PubMed  Google Scholar 

  • NCCLS (2002) Analysis and presentation of cumulative susceptibility test data: Approved Guideline. NCCLS document M39-A

  • Rafter J (2003) Probiotics and colon cancer. Best Pract Res Clin Gastroenterol 17:849–859

    Article  PubMed  Google Scholar 

  • Rönkä E, Malinen E, Saarela M, Rinta-Koski M, Aarnikunnas J, Palva A (2003) Probiotic and milk technological properties of Lactobacillus brevis. Int J Food Microbiol 83:63–74

    Article  PubMed  Google Scholar 

  • Saarela M, Mogensen G, Fonden R, Matto J, Mattila-Sandholm T (2000) Probiotic bacteria: safety, functional and technological properties. J Biotechnol 84:197–215

    Article  PubMed  CAS  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Article  PubMed  CAS  Google Scholar 

  • Sepp E, Jugle K, Vasar M, Naaber P, Bjørksten B, Mikelsaar M (1997) Intestinal microflora of Estonian and Swedish infants. Acta Pediatr 86:956–961

    Article  CAS  Google Scholar 

  • Servin LA (2004) Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiol Rev 28:405–440

    Article  PubMed  CAS  Google Scholar 

  • Shibatani T, Kakimoto T, Chibata I (1975) Crystallization and properties of l-arginine deiminase of Pseudomonas putida. J Biol Chem 250:4580–4583

    PubMed  CAS  Google Scholar 

  • Sun ZH, Zheng P, Ni Y, Liu YM, Liu YP (2007) An arginine deiminase producing strain and its application, Chinese patent (Application No. 200710107822.X)

  • Tagg JR, McGiven AR (1971) Assay system for bacteriocins. Appl Microbiol 21:943

    PubMed  CAS  Google Scholar 

  • Vinderola CG, Reinheimer JA (2003) Lactic acid starter and probiotic bacteria, a comparative “in vitro” study of probiotic characteristics and biological barrier resistance. Food Res Int 36:895–904

    Article  CAS  Google Scholar 

  • Vinderola CG, Perdigón G, Duarte J, Farnworth E, Matar C (2006) Effects of kefir fractions on innate imunity. Immunobiology 211:149–156

    Article  PubMed  CAS  Google Scholar 

  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703

    PubMed  CAS  Google Scholar 

  • Worthington Biochemical Corporation (1993) Worthington Enzyme Manual. Available from: http://www.worthingtonbiochem.com/index/manual.html. Accessed 20 August 2007

  • Yan F, Polk DB (2010) Probiotics: progress toward novel therapies for intestinal diseases. Curr Opin Gastroenterol 26:95–101

    Article  PubMed  Google Scholar 

  • Zuniga M, Perez G, Gonzalez-Candelas F (2002) Evolution of arginine deiminase (ADI) pathway genes. Mol Phylogenet Evol 25:429–444

    Article  PubMed  CAS  Google Scholar 

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Acknowledgment

This study was supported by National Research Center, Egypt

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Authors and Affiliations

  1. Department of Chemistry of Natural and Microbial Products, National Research Center, El Buhouth St., Dokki, Giza, Cairo, 12311, Egypt

    Mai N. Amer, Nahla M. Mansour & Ahmed I. El-Diwany

  2. Gut Microbiology and Immunology Group—Central of Excellence for Advanced Sciences (CEAS), National Research Center, El Buhouth St., Dokki, Giza, Cairo, 12311, Egypt

    Nahla M. Mansour

  3. Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt

    Insaf E. Dawoud & Ferial M. Rashad

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  1. Mai N. Amer
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  2. Nahla M. Mansour
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  3. Ahmed I. El-Diwany
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  4. Insaf E. Dawoud
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  5. Ferial M. Rashad
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Correspondence to Nahla M. Mansour.

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Amer, M.N., Mansour, N.M., El-Diwany, A.I. et al. Isolation of probiotic lactobacilli strains harboring l-asparaginase and arginine deiminase genes from human infant feces for their potential application in cancer prevention. Ann Microbiol 63, 1121–1129 (2013). https://doi.org/10.1007/s13213-012-0569-6

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  • DOI: https://doi.org/10.1007/s13213-012-0569-6

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