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Production and characterization of polygalacturonase from thermophilic Thermoascus aurantiacus on submerged fermentation

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Abstract

Microbial pectinase production by submerged fermentation (SMF) has been conducted mainly using mesophilic microorganisms. However, the use of thermophilic fungal strains capable of producing thermostable and thermophilic enzymes offers specific alternatives to biotechnology processes that use enzymes with such characteristics. Pectinases produced by thermophilic fungi may exhibit interesting characteristics, such as high thermostability, in terms of industrial application. Polygalacturonase (PG) production by the thermophilic fungus Thermoascus aurantiacus on SMF was carried out in culture media containing commercial pectin or agro-industrial by-products as carbon source under different fermentation conditions. The highest enzyme production occurred when the liquid waste from orange juice processing was used as substrate, after 5 days of cultivation. PG activity was highest when the fungus was cultivated at 45°C with an initial pH of 5.5. PG showed an optimum temperature of 60°C and optimum pH of 5.0. The enzyme showed greater stability in acid pH (3.0–4.5) and remained stable when incubated at 55°C for 1 h.

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Acknowledgment

The authors wish to thank Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for financial support.

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Authors and Affiliations

  1. Laboratory of Microbiology, Universidade do Estado de Minas Gerais (UEMG), Av. Prof. Mario Palmerio 1000, 38200-000, Frutal, MG, Brazil

    Eduardo da Silva Martins

  2. Biological and Environmental Sciences Faculty, FCBA, Universidade Federal da Grande Dourados (UFGD), Km 12, Rodovia Dourados—Itahum, Dourados, MS, 79804-970, Brazil

    Rodrigo Simões Ribeiro Leite

  3. Laboratory of Biochemistry and Applied Microbiology, Ibilce, Universidade Estadual Paulista (UNESP), Rua Cristovao Colombo, 2265, Jd. Nazareth, São José do Rio Preto, SP, 15054-000, Brazil

    Roberto da Silva & Eleni Gomes

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  1. Eduardo da Silva Martins
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  2. Rodrigo Simões Ribeiro Leite
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  3. Roberto da Silva
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  4. Eleni Gomes
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Correspondence to Eduardo da Silva Martins.

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Martins, E.d.S., Leite, R.S.R., da Silva, R. et al. Production and characterization of polygalacturonase from thermophilic Thermoascus aurantiacus on submerged fermentation. Ann Microbiol 62, 1199–1205 (2012). https://doi.org/10.1007/s13213-011-0360-0

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  • DOI: https://doi.org/10.1007/s13213-011-0360-0

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