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Diversity of nifH gene in rhizosphere and non-rhizosphere soil of tobacco in Panzhihua, China

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Abstract

To gain a better understanding of the effects of tobacco root secretions on the nifH gene community of rhizosphere soil, the diversity of the nifH gene in the rhizosphere and non-rhizosphere soil of tobacco was investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing. Shannon diversity (H), richness (S) and evenness (E H) indices were used to analyze the DGGE results. The results showed that the nifH gene diversity for rhizosphere soil was lower than that of the non-rhizosphere at three sampling sites. The increase in H of non-rhizosphere soil was due mainly to the increase in pH and the decrease in available N. Richness was correlated closely with H. There was a great difference of evenness between the rhizosphere and the non-rhizosphere samples. A range of sequence divergence was observed in the eight sequenced nifH clones. The sequences were divided into three clusters in the phylogenetic tree. The majority of the clones were similar to the nifH genes of Betaproteobacteria, Zoogloea oryzae, Dechloromonas sp. and Azovibrio restrictus. There was a difference regarding dominant species between the rhizosphere and the non-rhizosphere samples.

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Acknowledgments

The authors are grateful to an anonymous reviewer for kindly correcting this manuscript. This work was kindly funded by the Chinese Natural Science Foundation (no. 31070004) and the specialized research fund of the Doctoral Program of Higher Education 20060626006.

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Authors and Affiliations

  1. College of Resource and Environment, Sichuan Agricultural University, Yaan, 625014, China

    Xiaolin Li, Yunfu Gu & Xiaoping Zhang

  2. Department of Food and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland

    Petri Penttinen

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  1. Xiaolin Li
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  2. Petri Penttinen
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  3. Yunfu Gu
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  4. Xiaoping Zhang
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Correspondence to Xiaoping Zhang.

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Li, X., Penttinen, P., Gu, Y. et al. Diversity of nifH gene in rhizosphere and non-rhizosphere soil of tobacco in Panzhihua, China. Ann Microbiol 62, 995–1001 (2012). https://doi.org/10.1007/s13213-011-0339-x

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  • DOI: https://doi.org/10.1007/s13213-011-0339-x

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