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Biocontrol of olive knot disease by Bacillus subtilis isolated from olive leaves

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Abstract

We evaluated the biocontrol efficacy of a Bacillus subtilis strain isolated from symptomless olive leaves against the olive knot disease pathogen Pseudomonas savastanoi pv. savastanoi. Bacillus subtilis F1 and F-4 displayed antibacterial activity against the pathogen. In planta, only B. subtilis F1 significantly reduced the weight of knots caused by P. savastanoi pv. savastanoi IVIA 1628 and Aw9, whereas copper treatment was effective only when using strain Aw9, thus a reduction of 50% was obtained. Preliminary characterisation of the active compound produced by B. subtilis F1 showed that it was proteinaceous in nature. These results suggest that B. subtilis strain F1 could be used to control, and prevent infection by, the causal agent of olive knot disease.

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Acknowledgements

This work was supported by the funds of Institut de l’Olivier and Centre de Biotechnologie de Sfax, Tunisia.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, BP 1177, 3018, Sfax, Tunisia

    Samira Krid & Ali Gargouri

  2. Unité de Recherche Protection des Plantes Cultivées et Environnement, Institut de l’Olivier, Cité Mahrajène, BP 208, Tunis, Tunisia

    Samira Krid, Mohamed Ali Triki & Ali Rhouma

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  1. Samira Krid
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  2. Mohamed Ali Triki
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  3. Ali Gargouri
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  4. Ali Rhouma
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Correspondence to Samira Krid.

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Krid, S., Triki, M.A., Gargouri, A. et al. Biocontrol of olive knot disease by Bacillus subtilis isolated from olive leaves. Ann Microbiol 62, 149–154 (2012). https://doi.org/10.1007/s13213-011-0239-0

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  • DOI: https://doi.org/10.1007/s13213-011-0239-0

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