Abstract
A Gram positive bacterium obtained from a sediment sample from the alkaline Lonar Lake, India, was identified as Vagococcus carniphilus by morphological, physiological and biochemical characterisation and 16S rDNA sequencing. The bacterium grew in sodium chloride (NaCl) up to 2.5 M and at pH 7–11, 10 being optimum. Mucoid, glistening colonies were produced on exopolysaccharide (EPS) production medium (pH 10); the organism produced 1,126 mg l−1 EPS under optimized conditions. The gross chemical composition of Vagococcus EPS indicated 20% protein and 75% neutral sugars, while the monosaccharide composition revealed the presence of galactose and mannose with an additional three spots corresponding to unidentified compounds. FTIR analysis confirmed the presence of an alkyl group in the polysaccharide and revealed 75% similarity with standard dextran. Vagococcus carniphilus isolated from Lonar Lake was different from a previously reported strain with respect to its biotechnological potential for EPS production. In the present study, the newly isolated V. carniphilus from Lonar Lake was characterized and studied for production of EPS.
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Acknowledgements
The authors are thankful to the Director, ARI, for laboratory facilities. A.A.J. thanks the Council of Scientific and Industrial Research (CSIR), Govt. of India, for a Senior Research Fellowship. We also thank Dr. Rohit Sharma for revising our English.
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Joshi, A.A., Kanekar, P.P. Production of exopolysaccharide by Vagococcus carniphilus MCM B-1018 isolated from alkaline Lonar Lake, India. Ann Microbiol 61, 733–740 (2011). https://doi.org/10.1007/s13213-010-0189-y
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DOI: https://doi.org/10.1007/s13213-010-0189-y