Skip to main content
SpringerLink
Log in

Identification of Possibility of Glycyrrhiza uralensis as an Allergen by Protein Analysis

  • Original Article
  • Published:
BioChip Journal Aims and scope Submit manuscript

Abstract

Various secondary metabolites derived from plants are useful for humans to preserve their health. For this reason, medical herbs have been consumed universally without deep research about the dangerousness of these substance. However, they can produce unexpected adverse reactions in humans. Among these side effects, allergic reactions involving human immunoglobulin E can occur due to antimicrobial or antibacterial substances in plants. Glycyrrhiza uralensis has been prescribed for the treatment of numerous diseases given its multiple types of pharmaceutical efficacy, including its antimicrobial and antibacterial activities. Therefore, we researched whether Glycyrrhiza uralensis, which has antimicrobial ability, can cause allergic reaction in humans. To test the hypothesis devised here, we conducted proteomic level experiments. We utilized SDS-PAGE using a protein extract of Glycyrrhiza uralensis, with Coomassie blue staining to confirm the protein pattern and western blotting to identify Human IgE binding proteins in Glycyrrhiza uralensis. Among the many candidate sera, these methods detected three positive sera. Moreover, an identified protein section with reactivity associated with Human IgE was analyzed by LC-MS/ MS. Our experimental data provide a basis for testing the possibility of Glycyrrhiza uralensis as an allergen by identifying the reactivity between human Immunoglobulin E and proteins of Glycyrrhiza uralensis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sampedro, J. & Valdivia, E.R. New Antimicrobial Agents of Plant Origin. in Antimicrobial Compounds: Current Strategies and New Alternatives (eds. Villa, T.G. & Veiga-Crespo, P.) 83–114 (Springer Berlin Heidelberg, Berlin, Heidelberg, 2014).

    Chapter  Google Scholar 

  2. Park, J. & Jang, H.-J. Anti-diabetic effects of natural products an overview of therapeutic strategies. Mol. Cell. Toxicol. 13, 1–20 (2017).

    Article  CAS  Google Scholar 

  3. Jang, S.-A. et al. Gamma irradiation-induced liver injury and its amelioration by red ginseng extract. Mol. Cell. Toxicol. 13, 461–469 (2017).

    Article  CAS  Google Scholar 

  4. Nilnumkhum, A., Punvittayagul, C., Chariyakornkul, A. & Wongpoomchai, R. Effects of hydrophilic compounds in purple rice husk on AFB1-induced mutagenesis. Mol. Cell. Toxicol. 13, 171–178 (2017).

    Article  CAS  Google Scholar 

  5. Cowan, M.M. Plant Products as Antimicrobial Agents. Clin. Microbiol. Rev. 12, 564–582 (1999).

    CAS  Google Scholar 

  6. Phannasorn, W., Khanaree, C., Wongnoppavich, A. & Chewonarin, T. The effect of purple rice (Oryza sativa L. indica) extract on the inflammatory response in a colon cancer cell line and dextran sulfate-induced tumor promotion in the rat colon. Mol. Cell. Toxicol. 13, 433–442 (2017).

    CAS  Google Scholar 

  7. Middleton, E., Jr., Kandaswami, C. & Theoharides, T.C. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol. rev. 52, 673–751 (2000).

    CAS  Google Scholar 

  8. Cunha, B.A. Antibiotic Side Effects. Med. Clin. North Am. 85, 149–185 (2001).

    Article  CAS  Google Scholar 

  9. Kokoska, L., Polesny, Z., Rada, V., Nepovim, A. & Vanek, T. Screening of some Siberian medicinal plants for antimicrobial activity. J. Ethnopharmacol. 82, 51–53 (2002).

    Article  CAS  Google Scholar 

  10. Hoffmann-Sommergruber, K. Plant Allergens and Pathogenesis-Related Proteins. Int. arch. allergy immunol. 122, 155–166 (2000).

    Article  CAS  Google Scholar 

  11. Sampson, H.A. Food anaphylaxis. BMJ 56, 925–935 (2000).

    CAS  Google Scholar 

  12. Asl, M.N. & Hosseinzadeh, H. Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds. Phytother. Res. 22, 709–724 (2008).

    CAS  Google Scholar 

  13. Gafner, S. et al. Isoflavonoids and Coumarins from Glycyrrhiza uralensis: Antibacterial Activity against Oral Pathogens and Conversion of Isoflavans into Isoflavan-Quinones during Purification. J. nat. prod. 74, 2514–2519 (2011).

    Article  CAS  Google Scholar 

  14. Zhao, Z., Wang, W., Guo, H. & Zhou, D. Antidepressant-like effect of liquiritin from Glycyrrhiza uralensis in chronic variable stress induced depression model rats. Behavioural Brain Research 194, 108–113 (2008).

    Article  CAS  Google Scholar 

  15. Gruchalla, R.S. & Pirmohamed, M. Antibiotic Allergy. N. Engl. J. Med. 354, 601–609 (2006).

    Article  CAS  Google Scholar 

  16. Kim, K.-H. et al. Isolated protein of Astragalus membranaceus acts as an allergen by binding human immunoglobulin E on human sera. BioChip J. 10, 95–102 (2016).

    Article  CAS  Google Scholar 

  17. Kim, K.-H., Park, J.Y., Lee, I.-S., Kim, Y. & Jang, H.-J. Proteins derived from Prunus armeniaca kernel are possible to cause Immunoglobulin E reactivity in human sera. Mol. Cell. Toxicol. 13, 213–220 (2017).

    Article  CAS  Google Scholar 

  18. Li, S., Geng, F., Wang, P., Lu, J. & Ma, M. Proteome analysis of the almond kernel (Prunus dulcis). J. sci. food agric. 96, 3351–3357 (2016).

    Article  CAS  Google Scholar 

  19. Al-Ghouleh, A. et al. The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent. PLoS One 7, e33929 (2012).

    Article  CAS  Google Scholar 

  20. Nierman, W.C. et al. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature 438, 1151–1156 (2005).

    Article  CAS  Google Scholar 

  21. Bishop, J.G., Dean, A.M. & Mitchell-Olds, T. Rapid evolution in plant chitinases: molecular targets of selection in plant-pathogen coevolution. Proc Natl Acad Sci U S A 97, 5322–5327 (2000).

    Article  CAS  Google Scholar 

  22. Yagami, T. [Plant defense-related proteins as latex allergens]. Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo hokoku=Bulletin of National Institute of Health Sciences, 46–62 (1998).

    Google Scholar 

  23. Yagami, T., Sato, M., Nakamura, A. & Shono, M. One of the rubber latex allergens is a lysozyme. J. allergy clin. immunol. 96, 677–686 (1995).

    Article  CAS  Google Scholar 

  24. Proteau, P.J. 1-Deoxy-d-xylulose 5-phosphate reductoisomerase: an overview. Bioorg. Chem. 32, 483–493 (2004).

    Article  CAS  Google Scholar 

  25. Takahashi, S., Kuzuyama, T., Watanabe, H. & Seto, H. A 1-deoxy-d-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-d-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis. Proc. Natl. Acad. Sci. 95, 9879–9884 (1998).

    Article  CAS  Google Scholar 

  26. Bailey, C.J. & Day, C. Traditional Plant Medicines as Treatments for Diabetes. Diabetes Care 12, 553–564 (1989).

    Article  CAS  Google Scholar 

  27. van Vuurden, D.G. et al. PARP inhibition sensitizes childhood high grade glioma, medulloblastoma and ependymoma to radiation. Oncotarget 2, 984–996 (2011).

    Google Scholar 

  28. Kim, K.-W. et al. Osteogenic differentiation of human mesenchymal stem cells promoted by the crude extracts of the mixture of Cortex mori radicis, Patrinia saniculaefolia. mol. Cell. Toxicol. 11, 475–482 (2015).

    Article  CAS  Google Scholar 

  29. Lee, S.Y. et al. Heterogeneous expression of Chla mydia pneumoniae antigen candidates and high-level soluble expression of its inclusion membrane proteins in Escherichia coli. Mol. Cell. Toxicol. 13, 387–394 (2017).

    Article  CAS  Google Scholar 

  30. Kang, H., Park, B.-R., Yoo, H.-S., Kwon, K.-R. & Kang, I.-C. Anti-angiogenic function of a Korean Ginseng and Toad venom complex, Doksamsumsu-dan (DSSSD) analyzed by a forwarded phase antibody microarray. BioChip J. 9, 222–231 (2015).

    Article  CAS  Google Scholar 

  31. Bahk, Y.Y. et al. Antigens secreted from Mycobacterium tuberculosis: identification by proteomics approach and test for diagnostic marker. Proteomics 4, 3299–3307 (2004).

    Article  CAS  Google Scholar 

  32. Gobom, J., Nordhoff, E., Mirgorodskaya, E., Ekman, R. & Roepstorff, P. Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry. J. mass spectrom. 34, 105–116 (1999).

    Article  CAS  Google Scholar 

  33. Byeon, J., Kang, K.H., Jung, H.-K. & Suh, J.-K. Assessment for quantification of biopharmaceutical protein using a microvolume spectrometer on microfluidic slides. BioChip J. 11, 21–29 (2017).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Graduate School, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Eun-Jin An, Kang-Hoon Kim, In-Seung Lee, Ji Young Park, Yumi Kim & Hyeung-Jin Jang

  2. Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Eun-Jin An, Kang-Hoon Kim, In-Seung Lee, Ji Young Park, Yumi Kim, Woo Sang Jung & Hyeung-Jin Jang

  3. Department of Microbiology, Catholic Kwandong University, College of Medicine, Gangneung, 25601, Republic of Korea

    Daeho Kwon

Authors
  1. Eun-Jin An
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kang-Hoon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. In-Seung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ji Young Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yumi Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Woo Sang Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Daeho Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hyeung-Jin Jang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Daeho Kwon or Hyeung-Jin Jang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

An, EJ., Kim, KH., Lee, IS. et al. Identification of Possibility of Glycyrrhiza uralensis as an Allergen by Protein Analysis. BioChip J 12, 75–82 (2018). https://doi.org/10.1007/s13206-017-2110-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13206-017-2110-2

Keywords

Search

Navigation