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Silver nanoparticle synthesis and their potency against multidrug-resistant bacteria: a green approach from tissue-cultured Coleus forskohlii

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Abstract

Drug resistance is a major concern nowadays, and finding alternatives of the well-known antibiotic is necessary. Green nanoparticles are emerging as a tenable alternative to this with a large spectrum of activity. The present manuscript describes an eco-friendly approach for green synthesis of silver nanoparticles from both in vitro and in vivo leaf extract of Coleus forskohlii. Leaf extracts were used in synthesis of nanoparticles which were further analyzed through UV–Vis, dynamic light scattering, energy-dispersive spectroscopy, and transmission electron microscopy. Antimicrobial activity of silver nanoparticles alone, as well as crude extract of the plant itself, was carried out against eight multidrug-resistant respiratory tract infecting pathogenic strains. Satisfactory antimicrobial activities were found with nanoparticles, in vitro and in vivo leaf extracts. However, gradually higher to lower inhibition potential against pathogenic bacterial strains was found in silver nanoparticles, in vitro and in vivo leaf extracts. Seven bioactive compounds were detected in the crude extract through gas chromatography–mass spectroscopy analysis. Results revealed that nanoparticle formation occurred in a wide range of sizes (10–50 nm) and shapes (trigonal, hexagonal, spherical, rod). The diversity in size and shape of the nanoparticles makes them biologically active. Silver nanoparticle exhibits significantly better antimicrobial activities as compared to the plant extract in case of nearly all pathogens with a maximum zone of inhibition of 15.33 ± 0.94 mm where more than 12 well-known antibiotics failed to respond. Because of this broad-spectrum activity of nanoparticles as well as the leaf extracts against life-threatening microbes, it can be used as future generation drugs.

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Acknowledgements

AC, SMH and BG thankful to Swami Kamalasthananda, Principal, Ramakrishna Mission Vivekananda Centenary College, Rahara, Kolkata (India), for the facilities provided for the present study and Centre for research in nanoscience and nanotechnology, University of Calcutta for the use of transmission electron microscope. DD acknowledges technical and management support provided by Ashok Laboratory Clinical Testing Centre Private Limited, Kolkata.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Post Graduate Department of Botany, Ramakrishna Mission Vivekananda Centenary College, Rahara, Kolkata, 700118, India

    Avijit Chakraborty, Sk Moquammel Haque & Biswajit Ghosh

  2. Department of Botany, East Calcutta Girls’ College, Lake Town, Kolkata, 700089, India

    Sk Moquammel Haque

  3. Department of Chemistry, University of Calcutta, University College of Science, 92, A. P. C Road, Kolkata, 700009, India

    Debasish Ghosh & Dilip K. Maity

  4. Department of Microbiology, Ashok Laboratory Clinical Testing Centre Private Limited, Kolkata, 700068, India

    Diganta Dey

  5. Department of Microbiology, Dinabandhu Andrews College, Garia, Kolkata, 700084, India

    Swapna Mukherjee

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  1. Avijit Chakraborty
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  7. Biswajit Ghosh
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Contributions

AC screened the antimicrobial activities and GC–MS of plant extract and EDS, TEM study of nanoparticle and write up a part of manuscript. SMH performed all the plant tissue culture related experiments and write up a part of manuscript. DD collected the clinical isolates and checked their response against multidrug. DG performed DLS under the guidance of DM. SM and BG was involved in result interpretation and made necessary correction in the write up. Final approval of the article was done by BG. All authors read and approved the final manuscript.

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Correspondence to Biswajit Ghosh.

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Chakraborty, A., Haque, S.M., Ghosh, D. et al. Silver nanoparticle synthesis and their potency against multidrug-resistant bacteria: a green approach from tissue-cultured Coleus forskohlii. 3 Biotech 12, 228 (2022). https://doi.org/10.1007/s13205-022-03295-z

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