Abstract
Gossypium hirsutum L. represents the best cotton species for fiber production, thus computing the largest cultivated area worldwide. Meloidogyne incognita is a root-knot nematode (RKN) and one of the most important species of Meloidogyne genus, which has a wide host range, including cotton plants. Phytonematode infestations can only be partially controlled by conventional agricultural methods, therefore, more effective strategies to improve cotton resistance to M. incognita disease are highly desirable. The present study employed functional genomics to validate the involvement of two previously identified candidate genes, encoding dirigent protein 4—GhDIR4 and peroxiredoxin-2—GhPRXIIB, in cotton defense against M. incognita. Transgenic A. thaliana plant lines overexpressing GhDIR4 and GhPRXIIB genes were generated and displayed significantly improved resistance against M. incognita infection in terms of female nematode abundance in the roots when compared to wild-type control plants. For our best target-gene GhDIR4, an in-silico functional analysis, including multiple sequence alignment, phylogenetic relationship, and search for specific protein motifs unveiled potential orthologs in other relevant crop plants, including monocots and dicots. Our findings provide valuable information for further understanding the roles of GhDIR and GhPRXIIB genes in cotton defense response against RKN nematode.
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Acknowledgements
This study was financed by Embrapa, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), INCT-PlantStress Biotech, and FAPDF.
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CS, LSTC, RFCL, PNM, LBDL and MELS performed the experiments. FTPKT performed statistical analysis and wrote the manuscript. MFGS supervised the work. AM conceived the idea, obtained the resources and was in charge of overall supervision and project administration. All authors read and approved the final manuscript.
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Additional file 1: Table S1.
List of primer pairs used in the study.
Additional file 2: Figure S1.
Molecular main steps involved in the construction of plasmid vector pK7WGF2 used in the study for gene overexpression.
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dos Santos, C., Carmo, L.S.T., Távora, F.T.P.K. et al. Overexpression of cotton genes GhDIR4 and GhPRXIIB in Arabidopsis thaliana improves plant resistance to root-knot nematode (Meloidogyne incognita) infection. 3 Biotech 12, 211 (2022). https://doi.org/10.1007/s13205-022-03282-4
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DOI: https://doi.org/10.1007/s13205-022-03282-4