Abstract
Arsenic and chromium are the most common environmental toxicants prevailing in nature. Hence, the present study endeavors to investigate the salutary effects of Coenzyme Q10 (CoQ10), Biochanin A (BCA), and Phloretin (PHL) on the combined neurotoxic impact of arsenic and chromium in the Swiss albino mice (Mus musculus). Sodium meta-arsenite (100 ppm) and potassium dichromate (75 ppm) were given orally in conjugation with CoQ10 (10 mg/kg), BCA & PHL (50 mg/kg each) in accordance with body weight per day for the 2 weeks experimental duration. Weight reduction was figured out in the exposed toxic group of arsenic and chromium in contrast with the comparison group (control), and with the selected anti-oxidants treatment, it rose significantly to the basal status (p < 0.05). The concentration of arsenic and chromium was reduced significantly (p < 0.001) amidst all the natural compounds co-medicated groups. Anti-oxidant indicators, viz. lipid peroxidation (LPO) and protein carbonyl content (PCC), were found elevated, with reduction observed in the levels of superoxide dismutase (SOD), reduced glutathione (GSH), glutathione s-transferase (GST), and total thiols (TT) in the arsenic and chromium, co-exposed mice. The alterations in redox homeostasis were well corroborated with the estimations of cholinesterase’s enzymes (p < 0.05) along with DNA fragmentation assay and altered Nrf2 signaling. The administration of CoQ10, BCA, and PHL ameliorated the effects of arsenic and chromium induced oxidative stress in the exposed mice. Our research unfolds the remedial outcome of these natural compounds contrary to the combined arsenic and chromium associated-neurotoxicity in the experimental model.
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Acknowledgements
The authors are thankful to the Director, ICMR-NIOH Ahmedabad, for conducting this study. The authors also acknowledge the assistance from Animal House Facility staff, especially Dr. Rajendra Palkhade, during the study. The authors are also grateful to the Zydus Research Center (ZRC), Ahmedabad, for providing the male Swiss albino mice for the study.
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ST: investigation, visualization, writing—original draft preparation. SF: investigation, visualization, DP: investigation, visualization, DPS and SM: resources, RM: investigation, resources, GS: conceptualization, visualization, supervision, writing—reviewing and editing.
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All the animal experimental procedures of the present study were conducted in accordance with the Institutional Animal Ethics Committee (IAEC) of the ICMR-National Institute of Occupational Health (NIOH), Ahmedabad wide approval no. IAEC/NIOH/2018–19/21/02/M.
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Tripathi, S., Fhatima, S., Parmar, D. et al. Therapeutic effects of CoenzymeQ10, Biochanin A and Phloretin against arsenic and chromium induced oxidative stress in mouse (Mus musculus) brain. 3 Biotech 12, 116 (2022). https://doi.org/10.1007/s13205-022-03171-w
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DOI: https://doi.org/10.1007/s13205-022-03171-w