Abstract
With the increasing growth of the herbal market, a rapid and easy-to-use system is highly desirable in the high-throughput identification of massive herbal medicine samples. Here, an ultrafast and colorimetric detection system was devised based on simplifying template preparation and a newly developed amplification technique, named colorimetric direct-VPCR. The system was successfully applied to the identification of Pinelliae Rhizoma. Compared to the traditional method, the whole test can be finished within 30 min from the sample treatment to the testing results. The method was evaluated by correctly identifying 72 samples obtained from 9 different habitats, demonstrating its high reliability. In summary, we present an ultrafast (less than 30 min) and colorimetric detection platform (under ultraviolet lamp) based on direct-VPCR for the identification of Pinelliae Rhizoma. The high practicability (100% accuracy) of this pipeline enables it to be a promising method in the routine detection of other herbal materials.
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Funding
This work was supported by the National Natural Science Foundation of China [Grant number 81973434], Health Commission of Sichuan Province [Grant number 20PJ165], Xinglin Scholar Research Premotion Project of Chengdu University of TCM [Grant number 18TD0017].
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Chen, R., Ding, S., Wei, Y. et al. Ultrafast identification of Pinelliae Rhizoma using colorimetric direct-VPCR. 3 Biotech 11, 493 (2021). https://doi.org/10.1007/s13205-021-03035-9
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DOI: https://doi.org/10.1007/s13205-021-03035-9