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Assessment of α-amylase and α-glucosidase inhibitory potential of Citrus reticulata peel extracts in hyperglycemic/hypoglycemic rats

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Abstract

Diabetes mellitus is a metabolic disorder of carbohydrate metabolism. The management of Diabetes mellitus with phytochemicals is hallmark of this research. Citrus species are known for their health benefits and are used as traditional food in South East Asia. The total phenolic content of peels was analyzed using different solvents, while Gallic acid was used as standard. Both ethanolic, aqueous extracts of Citrus reticulata peel showed good inhibitory activity against amylase (90.67%, 15.33%) and moderate against glucosidase (70.8%, 14.8%), respectively. Sixteen rats were randomly divided into four groups (G1, G2, G3, and G4); G1 is a negative control (water), G4 is a positive control (Acarbose), while other two are experimental groups like G2 (fed with 100 mL and 20 mg/mL in hypoglycemic and hyperglycemic trials) and G3 fed with 200 mL and 40 mg/mL in hypoglycemic and hyperglycemic trials. A significant effect of treatments and value of time was found in hyperglycemic rats. Ethanolic extract showed a significant reduction in blood glucose levels in hypoglycemic (overnight fasting) rats which was comparable to the positive control. These results suggest that C. reticulata peels can contribute as a useful food ingredient as a potential antihyperglycemic agent in managing type 2 diabetes mellitus. In future, C. reticulata peel will be a good candidate for pharmaceutical industry.

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Acknowledgements

We are thankful to Director IBBT, Prof. Dr. Wasim Shahzad for providing great support to conduct this research project.

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Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, The University of Veterinary and Animal Sciences, Lahore, Pakistan

    Sanaid Ghauri, Syed Qasim Raza, Muhammad Imran, Shagufta Saeed & Rahat Naseer

  2. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan

    Muhammad Rashid

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  1. Sanaid Ghauri
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  4. Shagufta Saeed
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Correspondence to Rahat Naseer.

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The research was conducted after approval from independent ethical committee UVAS vide letter no. 118/BeST center 18.

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Ghauri, S., Raza, S.Q., Imran, M. et al. Assessment of α-amylase and α-glucosidase inhibitory potential of Citrus reticulata peel extracts in hyperglycemic/hypoglycemic rats. 3 Biotech 11, 167 (2021). https://doi.org/10.1007/s13205-021-02717-8

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