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Functional characterization of tau class glutathione-S-transferase in rice to provide tolerance against sheath blight disease

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Abstract

Glutathione-S-transferase (GST) is an important defense gene that confers resistance against several abiotic and biotic stresses. The present study identifies a tau class GST in rice (Oryza sativa L.), OsGSTU5 (Os09g20220), which provided tolerance against sheath blight (SB) disease, caused by a necrotrophic fungus, Rhizoctonia solani (RS). Overexpression and knockdown rice transgenic lines of OsGSTU5 were generated and tested for the severity of infection during sheath blight disease. The results obtained after RS infection showed that the lesion cover area and hyphal penetration were more in knockdown line and lesser in the overexpression line. Analysis of reactive oxygen species (ROS) accumulation showed more spots of H2O2 and O2− in knockdown lines compared to overexpressed lines. Later, RS transcript level was analyzed in RS-infected transgenic lines, which manifested that the knockdown line had higher RS transcripts in comparison to the control line and least RS transcripts were observed in the overexpressed line. In conclusion, rice transgenic lines overexpressing OsGSTU5 were found to be more tolerant, while the knockdown lines were more prone to Rhizoctonia infection compared to control lines.

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Acknowledgements

We are thankful to Director CSIR-NBRI for the lab facilities provided during this work. The author is thankful to Banaras Hindu University (BHU) for the registration (Sept.2014/271) and UGC for fellowship. We acknowledge the financial support of CSIR in-house Project OLP-0104. The author is also thankful to Dr. Emmanuel Guiderdoni, CIRAD, France, for pIRS vector. This manuscript bears NBRI Communication Number CSIR-NBRI_MS/2020/01/01.

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Authors and Affiliations

  1. CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India

    Madhu Tiwari, Suchi Srivastava, Poonam C. Singh & Debasis Chakrabarty

  2. Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Madhu Tiwari & Arun Kumar Mishra

  3. Academy of Scientific and Innovative Research, Gaziabad, India

    Suchi Srivastava, Poonam C. Singh & Debasis Chakrabarty

Authors
  1. Madhu Tiwari
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  2. Suchi Srivastava
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  3. Poonam C. Singh
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  4. Arun Kumar Mishra
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  5. Debasis Chakrabarty
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Contributions

DC planned, supervised the experiments, and also reviewed the manuscript. MT performed the experiments, executed data analysis, and wrote the manuscript. AKM supervised during the work. SS and PCS helped during the experimental work.

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Correspondence to Debasis Chakrabarty.

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Tiwari, M., Srivastava, S., Singh, P.C. et al. Functional characterization of tau class glutathione-S-transferase in rice to provide tolerance against sheath blight disease. 3 Biotech 10, 84 (2020). https://doi.org/10.1007/s13205-020-2071-3

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  • DOI: https://doi.org/10.1007/s13205-020-2071-3

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