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In vitro bioactivity, antimicrobial and anti-inflammatory efficacy of modified solvent evaporation assisted Trametes versicolor extract

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Abstract

In the present study, modified solvent evaporation assisted methanolic Trametes versicolor extract was evaluated for its antimicrobial and anti-inflammatory efficacy. Mushroom extract showed significantly (p < 0.05) higher total phenolic content (48.71 mg/g) followed by total flavonoid content (13.13 mg/g), ascorbic acid content (11.03 mg/g), β-carotene content (8.34), and lycopene content (6.85). Fourier transform infrared spectroscopy revealed the functional groups of the observed bioactive compounds, while HPLC chromatogram showed significantly (p < 0.05) higher gallic acid content (45.72 mg/g) as compared to rutin content (12.50 mg/g). Statistically, mushroom extract and artificial antioxidant (BHA) showed a non-significant (p < 0.05) difference in terms of percentage inhibition during DPPH and N2O2 scavenging assay. During the time-kill kinetics, the mushroom extract significantly inhibited the growth of S. aureus in comparison with the growth of P.aeruginosa, K. pneumonia, and E.coli. Mushroom extract showed effective anti-inflammatory activity during membrane stabilization (33.71–73.24%) and protein denaturation (23.11–74.56%) assays.

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Abbreviations

DPPH:

2,2 Diphenyl-1-picrylhydrazyl

N2O2 :

Nitric oxide

BHA:

Butylated hydroxyanisole

HPLC:

High performance liquid chromatography

FTIR:

Fourier transform infrared spectroscopy

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Acknowledgements

Authors are grateful acknowledged to Shoolini University, Solan, Himachal Pradesh

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Authors and Affiliations

  1. Department of Biotechnology, Chandigarh Group of Colleges Landran, Mohali, 140307, Punjab, India

    Aarti Bains

  2. School of Agriculture, Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, 144411, Punjab, India

    Prince Chawla

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  1. Aarti Bains
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AB did all practical and Laboratory work. PC in writing paper provided the research idea.

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Correspondence to Prince Chawla.

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There is no conflict of interest between the authors.

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No animal trial was done during this research project.

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Bains, A., Chawla, P. In vitro bioactivity, antimicrobial and anti-inflammatory efficacy of modified solvent evaporation assisted Trametes versicolor extract. 3 Biotech 10, 404 (2020). https://doi.org/10.1007/s13205-020-02397-w

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