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A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering

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Abstract

Antifreeze proteins (AFPs) confer the ability to survive at subzero temperatures and are found in many different organisms, including fish, plants, and insects. They prevent the formation of ice crystals by non-colligative adsorption to the ice surface and are essential for the survival of organisms in cold environments. These proteins are also widely used for cryopreservation, food technology, and metabolic genetic engineering over a range of sources and recipient cell types. This review summarizes successful applications of AFPs in the cryopreservation of animals, insects, and plants, and discusses challenges encountered in cryopreservation. Applications in metabolic genetic engineering are also described, specifically with the overexpression of AFP genes derived from different organisms to provide freeze protection to sensitive crops seasonally exposed to subzero temperatures. This review will provide information about potential applications of AFPs in the cryopreservation of animals and plants as well as in plant metabolic genetic engineering in hopes of furthering the development of cold-tolerant organisms.

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Funding

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Agri-Bio industry Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant# 315002-5)

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  1. Department of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University, Daegu, 41566, Korea

    Aung Htay Naing & Chang Kil Kim

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AHN collected the literature and wrote the manuscript; CKK advised and assisted with the writing of the manuscript.

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Correspondence to Chang Kil Kim.

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Naing, A.H., Kim, C.K. A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering. 3 Biotech 9, 329 (2019). https://doi.org/10.1007/s13205-019-1861-y

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  • DOI: https://doi.org/10.1007/s13205-019-1861-y

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