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Bio-production of novel water-soluble yellow pigment from Aspergillus sp. and exploring its sustainable textile applications

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Abstract

In the present study, 40 pigment-producing microbes were isolated from various soil sources. Among these, a novel water-soluble yellow pigment-producing fungal isolate (MBYP1) was identified as Aspergillus sp. through ITS gene sequencing. The maximum pigment yield (UA430nm, 12.45 ± 0.5 g/l) was obtained when strain MBYP1 was cultured under optimum conditions (28 °C and pH 5.5 under static condition). Subsequently, the pigment was purified through gel chromatography and high-performance liquid chromatography (HPLC). Characterization of purified pigment through UV–Vis and liquid chromatography–mass spectrometry (LC–MS) reveal maximum absorbance at 430 nm and molecular mass of 301 m/z, respectively. Further, the pigment exhibited a maximum dyeing capacity of up to 80% irrespective of mordant. Toxicity evaluation of purified pigment with zebra fish model system reported an IC50 value of 710 µg/mL. Pigment antioxidant ability was established by DPPH (35.7 µg/mL) and phosphomolybdenum assay (226.61 mg/g) thus ascertaining improvised light fastness of dyed fabric. Moreover, lack of antimicrobial activity (up to 40 µg/mL) improves pigment bio-degradability. In collective, the novel yellow pigment from Aspergillus sp. MBYP1 strain was found to be an eco-friendly alternative to synthetic dye for potential applications in textile industries.

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Acknowledgements

The authors are sincerely grateful to the Defense Research and Development Organization (DRDO), for financial support through Phase II project DRDO-BU CLS, Coimbatore, India. We also acknowledge DFRL, Mysore for great support.

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Authors and Affiliations

  1. DRDO-BU CLS, Bharathiar University Campus, Coimbatore, Tamil Nadu, 641 046, India

    S. Pandiyarajan & K. Kadirvelu

  2. Department of Nanoscience and Technology, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    P. Premasudha

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  1. S. Pandiyarajan
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  2. P. Premasudha
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  3. K. Kadirvelu
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PP and KK designed the experiment. SP performed the experiments, analyzed the data and prepared the manuscript.

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Correspondence to P. Premasudha.

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Pandiyarajan, S., Premasudha, P. & Kadirvelu, K. Bio-production of novel water-soluble yellow pigment from Aspergillus sp. and exploring its sustainable textile applications. 3 Biotech 8, 398 (2018). https://doi.org/10.1007/s13205-018-1424-7

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  • DOI: https://doi.org/10.1007/s13205-018-1424-7

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