Skip to main content

Advertisement

SpringerLink
Log in

Antimicrobial and anticancer activity of AgNPs coated with Alphonsea sclerocarpa extract

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

The synthesis and characterization of an aggregate of AgNPs coated with plant extract (PE) from Alphonsea sclerocarpa and its significant antimicrobial activity and inhibition on K562 (blood cancer) cells have been appended in the article. Synthesis of aggregate [(AgNPs)-(PE)] has been followed by a facile eco-friendly approach without using any harmful chemicals. The morphology of an aggregate [(AgNPs)-(PE)] was confirmed by TEM and SEM microscopic characterizations. Properties like solid state, the presence of functional groups, and elemental composition have been characterized through the XRD, FTIR, and EDAX. The biocompatibility of synthesized aggregate of [(AgNPs)-(PE)] was confirmed by the MTT assay. An in vitro cell (HEK293)-based studies were performed for the biocompatibility tests and it is found that the aggregate [(AgNPs)-(PE)] is not harmful to normal/healthy cells. Even though A. sclerocarpa show the antimicrobial (antibacterial and antifungal) activity, it has been further enhanced with the developed aggregate of [(AgNPs)-(PE)]. Furthermore, it has been extended to examine the cellular inhibition on K562 cells and obtained > 75% cell inhibition for 24 h treated cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Ahmed S, Ahmad M, Swami BL, Ikram S (2016) A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 7:17–28

    Article  CAS  Google Scholar 

  • Amgoth C, Dharmapuri G, Kalle AM, Paik P (2016) Nanoporous capsules of block copolymers of [(MeO-PEG-NH)-b-(L-GluA)]-PCL for the controlled release of anticancer drugs for therapeutic applications. Nanotechnology 27(12):125101

    Article  Google Scholar 

  • Andrews JM (2001) Determination of minimum inhibitory concentration. J Antimicrob Chemother 48(1):5–16

    Article  CAS  Google Scholar 

  • Dey Biswajit, Saha Rajat, Mukherjee Priyanka (2013) A luminescent-water soluble inorganic co-crystal for a selective pico- molar range arsenic (III) sensor in a water medium. Chem Commun 63:7064–7066

    Article  Google Scholar 

  • El-Chaghaby GA, Ahmad AF (2011) Biosynthesis of silver nanoparticles using Pistacia lentiscus leaves extract and investigation of their antimicrobial effect. Orient J Chem 27(3):929–936

    CAS  Google Scholar 

  • Eloff JN (1998) A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 64:711–713. https://doi.org/10.1055/s-2006-957563

    Article  CAS  Google Scholar 

  • Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on E. coli and Staphylococcus aureus. J Biomed Mater Res 52(4):662–668

    Article  CAS  Google Scholar 

  • Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650. https://doi.org/10.1039/c1gc15386b

    Article  CAS  Google Scholar 

  • Iravani S, Zolfaghari B (2013) Green synthesis of silver nanoparticles using Pinus eldarica bark extract. Biomed Res Int 2013:639725. https://doi.org/10.1155/2013/639725

    Article  Google Scholar 

  • Jang SJ, Yang IJ, Tettey CO, Kim KM, Shin HM (2016) In-vitro anticancer activity of green synthesised silver nanoparticles on MCF-7 human breast cancer cells. Mater Sci Eng 68:430–435. https://doi.org/10.1016/j.msec.2016.03.101

    Article  CAS  Google Scholar 

  • Korbekandi H, Iravani S, Abbasi S (2009) Production of nanoparticles using organisms. Crit Rev Biotechnol 29:279–306. https://doi.org/10.3109/07388550903062462

    Article  CAS  Google Scholar 

  • Krishnan R, Arumugam V, Vasaviah SK (2015) The MIC and MBC of silver nanoparticles against Enterococcus faecalis—a facultative anaerobe. J Nanomed Nanotechnol 6(3):285. https://doi.org/10.4172/2157-7439.1000285

    Google Scholar 

  • Kumar VA, Uchida T, Mizuki T, Nakajima Y, Katsube Y, Hanajiri T, Maekawa T (2016) Synthesis of nanoparticles composed of silver and silver chloride for a plasmonic photocatalyst using an extract from a weed Solidago altissima (goldenrod). Adv Nat Sci 7(1):015002

    Google Scholar 

  • Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H, Tam PK, Chiu JF, Che CM (2006) Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteome Res 5(4):916–924. https://doi.org/10.1021/pr0504079

    Article  CAS  Google Scholar 

  • Ma CY, Rout MK, Mock WY (2001) Study of oat globulin conformation by Fourier transform infrared spectroscopy. J Agric Food Chem 49:3328–3334

    Article  CAS  Google Scholar 

  • Mallmann EJ, Cunha FA, Castro BN, Maciel AM, Menezes EA, Fechine PB (2015) Antifungal activity of silver nanoparticles obtained by green synthesis. Rev Inst Med Trop Sao Paulo 57(2):165–177. https://doi.org/10.1590/S0036-46652015000200011

    Article  CAS  Google Scholar 

  • McFarland J (1907) Nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J Am Med Assoc 14:1176–1178. https://doi.org/10.1001/jama.1907.25320140022001f

    Article  Google Scholar 

  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ (2005) The bacterial effect of silver nanoparticles. Nanotechnology 16(10):2346–2353. https://doi.org/10.1088/0957-4484/16/10/059

    Article  CAS  Google Scholar 

  • Mueller NC, Nowack B (2008) Exposure modelling of engineered nanoparticles in the environment. Environ Sci Technol 42:4447–4453

    Article  CAS  Google Scholar 

  • Nagababu P, Uma Maheswara Rao V (2017) Pharmacological assessment, green synthesis and characterization of silver nanoparticles of sonneratia apetala Buch.-Ham. leaves. J App Pharm Sci 7(8):175–182

    Google Scholar 

  • Narayanan KB, Park HH (2014) Antifungal activity of silver nanoparticles synthesised using turnip leaf extract (Brassica rapa L.) against wood rotting pathogens. Eur J Plant Pathol 140:185–192. https://doi.org/10.1007/s10658-014-0399-4

    Article  CAS  Google Scholar 

  • Petrovska BB (2012) Historical review of medicinal plants’ usage. Pharmacogn Rev 6(11):1–5. https://doi.org/10.4103/0973-7847.95849

    Article  Google Scholar 

  • Prasad DN (2009) Antioxidant activity of Alphonsea sclerocarpa bark. Res J Pharmacol Pharmacodynamics 1(2):66–69

    Google Scholar 

  • Protima R, Siim K, Stanislav F, Erwan R (2015) A review on the green synthesis of silver nanoparticles and their morphologies studied via TEM. Adv Mat Sci Eng. https://doi.org/10.1155/2015/682749

    Google Scholar 

  • Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27(1):76–83. https://doi.org/10.1016/j.biotechadv.2008.09.002

    Article  CAS  Google Scholar 

  • Sastry M, Mayya KS, Bandyopadhyay K (1997) pH-dependent changes in the optical properties of carboxylic acid derivatised silver colloidal particles. Colloids Surf A 127(1–3):221–228. https://doi.org/10.1016/S0927-7757(97)00087-3

    Article  CAS  Google Scholar 

  • Sastry M, Patil V, Sainkar SR (1998) Electrostatically controlled diffusion of carboxylic acid derivatised silver colloidal particles in thermally evaporated fatty amine films. J Phy Chem B 102(8):1404–1410

    Article  CAS  Google Scholar 

  • Shrivastava S, Bera T, Roy A, Singh G, Rama Chandra Rao P, Dash D (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18:1–9

    Article  Google Scholar 

  • Siim K, Sander K, Protima R, Mithu G, David W, Erwan R (2016) Biocidal properties study of silver nanoparticles used for application in green housing. Int Nano Lett 6(3):191–197. https://doi.org/10.1007/s40089-016-0186-7

    Article  Google Scholar 

  • Silver S, Phung LT (1996) Bacterial heavy metal resistance: new surprises. Annu Rev Microbiol 50:753–789

    Article  CAS  Google Scholar 

  • Song HY, Ko KK, Oh IH, Lee BT (2006) Fabrication of silver nanoparticles and their antimicrobial mechanisms. Eur Cell Mater 11(1):58

    Google Scholar 

  • Suman Joshi DSD, Venkata Rao G, Satya Prasad M, Kishore Babu M, Surya Narayana S, Krishna Satya A (2017) Phytochemical screening and evaluation of antioxidant, antibacterial and antifungal activity of medicinal plant Alphonsea sclerocarpa Thaw. J Pharmacogn Phytochem 6(4):1280–1286

    Google Scholar 

  • Tacic D, Wannigama GP, Cassels BK, Cave A (1987) Alkaloids of Alphonsea sclerocarpa. J Nat Prod 50(3):518–519. https://doi.org/10.1021/np50051a036

    Article  Google Scholar 

  • Veerasamy R, Xin TZ, Gunasagaran S, Xiang TFW, Yang EFC, Jeyakumar N, Dhanaraj SA (2011) Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chem Soc 15:113–120. https://doi.org/10.1016/j.jscs.2010.06.004

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge DST-FIST, Department of Physics, Acharya Nagarjuna University for providing IR facility, University of Hyderabad, for providing SEM with EDAX and XRD facility.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, 522510, India

    Suman Joshi D S Doddapaneni, Surya Narayana Suryadevara & Krishna Satya Alapati

  2. School of Engineering Sciences and Technology, University of Hyderabad, Hyderabad, TS, 500046, India

    Chander Amgoth

  3. Department of Animal Sciences, School of Life sciences, University of Hyderabad, Hyderabad, TS, 500046, India

    Arunasree M Kalle

Authors
  1. Suman Joshi D S Doddapaneni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chander Amgoth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Arunasree M Kalle
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Surya Narayana Suryadevara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Krishna Satya Alapati
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Krishna Satya Alapati.

Ethics declarations

Conflict of interest

Both the first and second authors have equal contribution to the paper.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 6282 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Doddapaneni, S.J.D.S., Amgoth, C., Kalle, A.M. et al. Antimicrobial and anticancer activity of AgNPs coated with Alphonsea sclerocarpa extract. 3 Biotech 8, 156 (2018). https://doi.org/10.1007/s13205-018-1155-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-018-1155-9

Keywords

Search

Navigation