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Phenetic, genetic diversity and symbiotic compatibility of rhizobial strains nodulating pigeon pea in Northern India

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Abstract

Pigeon pea (Cajanus cajan) is one of the most important legumes grown in the northern province of Uttar Pradesh, India. However, its productively in Uttar Pradesh is lower than the average yield of adjoining states. During the course of the present study, a survey of pigeon pea growing agricultural fields was carried out and it was found that 80% of plants were inadequately nodulated. The study was aimed to evaluate the pigeon pea symbiotic compatibility and nodulation efficiency of root nodulating bacteria isolated from various legumes, and to explore the phenetic and genetic diversity of rhizobial population nodulating pigeon pea growing in fields of Uttar Pradesh. Amongst all the 96 isolates, 40 isolates showed nodulation in pigeon pea. These 40 isolates were further characterized by phenotypic, biochemical and physiological tests. Intrinsic antibiotic resistance pattern was taken to generate similarity matrix revealing 10 phenons. The study shows that most of the isolates nodulating pigeon pea in this region were rapid growers. The dendrogram generated using the NTSYSpc software grouped RAPD patterns into 19 clusters. The high degree of phenetic and genetic diversity encountered is probably because of a history of mixed cropping of legumes. The assessment of diversity is a very important tool and can be used to improve the nodulation and quality of pigeon pea crop. It is also concluded that difference between phenetic and RAPD clustering pattern is an indication that rhizobial diversity of pigeon pea is not as yet completely understood and settled.

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Acknowledgements

Authors are thankful to Prof H K Sehgal, former Vice Chancellor, CSJMU, Kanpur and NKA & ST are grateful to Vice Chancellor, Prof R C Sobti, BBA University for his support.

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Authors and Affiliations

  1. Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, 226025, India

    Naveen Kumar Arora

  2. Department of Microbiology, Institute of Biosciences and Biotechnology, C.S.J.M. University, Kanpur, 208024, India

    Ekta Khare & Sachin Singh

  3. Indian Institute of Technology, Delhi, New Delhi, 110016, India

    Sakshi Tewari

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  1. Naveen Kumar Arora
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Correspondence to Naveen Kumar Arora.

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Arora, N.K., Khare, E., Singh, S. et al. Phenetic, genetic diversity and symbiotic compatibility of rhizobial strains nodulating pigeon pea in Northern India. 3 Biotech 8, 52 (2018). https://doi.org/10.1007/s13205-017-1074-1

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