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Comparative lipidomics of drug sensitive and resistant Mycobacterium tuberculosis reveals altered lipid imprints

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Abstract

Lipids are most adaptable molecules that acclimatize to the development of multidrug resistance (MDR). The precise molecular mechanism of this acclimatization achieved in Mycobacterium tuberculosis (MTB) remains elusive. Although lipids of MTB have been characterized to some details, a comparable resource does not exist between drug sensitive (DS) and resistant (DR) strains of MTB. Here, by employing high-throughput mass spectrometry-based lipidomic approach, we attempted to analyze the differential lipidome profile of DS and DR MTB clinical isolates. We analyzed three major classes of lipids viz fatty acyls, glycerophospholipids and glycerolipids and their respective subclasses. Notably, we observed differential fatty acyls and glycerophospholipids as evident from increased mycolic acids phosphatidylinositol mannosides, phosphatidylinositol, cardiolipin and triacylglycerides abundance, respectively, which are crucial for MTB virulence and pathogenicity. Considering the fact that 30% of the MTB genome codes for lipid, this comprehensive lipidomic approach unravels extensive lipid alterations in DS and DR that will serve as a resource for identifying biomarkers aimed at disrupting the functions of MTB lipids responsible for MDR acquisition in MTB.

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Acknowledgements

We thank Dr. Sanjeev Kanojiya, Central Drug Research Institute (CDRI), Lucknow for assisting us in mass spectrometry experiments. We thank Dr. V. Sabareesh for his intellectual support in data analysis.

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Author notes

  1. Rahul Pal and Saif Hameed contributed equally to this work.

Authors and Affiliations

  1. Amity Institute of Biotechnology, Amity University Haryana, Manesar, Gurugram, 122413, India

    Rahul Pal, Saif Hameed & Zeeshan Fatima

  2. Division of Clinical Microbiology and Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, 110029, India

    Parveen Kumar & Sarman Singh

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  1. Rahul Pal
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  2. Saif Hameed
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  3. Parveen Kumar
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  4. Sarman Singh
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  5. Zeeshan Fatima
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Correspondence to Zeeshan Fatima.

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The authors declare that they have no competing interests.

Funding

Z.F. thanks Science and Engineering Research Board (SERB), New Delhi (SR/FT/LS-173/2010) for the financial assistance in the form of Young Scientist award.

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Pal, R., Hameed, S., Kumar, P. et al. Comparative lipidomics of drug sensitive and resistant Mycobacterium tuberculosis reveals altered lipid imprints. 3 Biotech 7, 325 (2017). https://doi.org/10.1007/s13205-017-0972-6

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