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Enzyme kinetics of cellulose hydrolysis of Miscanthus and oat hulls

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Abstract

Experiments were done to model enzymatic hydrolysis of Miscanthus and oat hulls treated with dilute solutions of nitric acid and sodium hydroxide in direct and reverse sequences. The enzymatic hydrolysis kinetics of the substrates was studied at an initial solid loading from 30 to 120 g/L. The effects of feedstock type and its pretreatment method on the initial hydrolysis rate and reducing sugar yield were evaluated. The fitting results by the developed models showed good agreement with the experimental data. These models designed for developing the production technology of concentrated glucose solutions can also be applied for glucose fermentation into ethanol. The initial solid loading of 60–90 g/L provides the reducing sugar concentration of 40–80 g/L necessary for ethanol synthesis. The kinetic model can also be applied to investigate enzymatic hydrolysis of other substrates (feedstock type, pretreatment method) under the similar conditions used herein, with adjusted empirical coefficient values.

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Abbreviations

CES :

Concentration of enzyme–substrate complex (g/L)

Cm :

Equilibrium concentration of reducing sugars (g/L)

CP :

Concentration of reducing sugars (g/L)

CS :

Concentration of substrate (g/L)

k1 :

Formation constant of enzyme–substrate complex (h−1)

k2 :

Breakdown constant of enzyme–substrate complex [g/(L h)]

k3 :

Formation constant of reducing sugars [g/(L h)]

km :

Michaelis constant (g/L)

ks :

Disassociation constant of enzyme–substrate complex (g/L)

NAM:

Nitric-acid method

CM:

Combined method

RS:

Reducing sugars

MC:

Miscanthus cellulose

OHC:

Oat hull cellulose

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Acknowledgements

This work was supported by the Federal Agency for Scientific Organizations of the Russian Federation [Grant No. 0385-2016-0001].

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Authors and Affiliations

  1. Laboratory of Bioconversion, Laboratory of Chemical Engineering Processes and Apparatuses, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), Biysk, Altai Krai, Russia, 659322

    Ekaterina I. Makarova, Vera V. Budaeva, Aleksey A. Kukhlenko & Sergey E. Orlov

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  1. Ekaterina I. Makarova
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  2. Vera V. Budaeva
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  3. Aleksey A. Kukhlenko
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  4. Sergey E. Orlov
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Correspondence to Ekaterina I. Makarova.

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Makarova, E.I., Budaeva, V.V., Kukhlenko, A.A. et al. Enzyme kinetics of cellulose hydrolysis of Miscanthus and oat hulls. 3 Biotech 7, 317 (2017). https://doi.org/10.1007/s13205-017-0964-6

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