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Evaluation of haplotype diversity of Achatina fulica (Lissachatina) [Bowdich] from Indian sub-continent by means of 16S rDNA sequence and its phylogenetic relationships with other global populations

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Abstract

Achatina fulica (Lissachatina fulica) is one of the most invasive species found across the globe causing a significant damage to crops, vegetables, and horticultural plants. This terrestrial snail is native to east Africa and spread to different parts of the world by introductions. India, a hot spot for biodiversity of several endemic gastropods, has witnessed an outburst of this snail population in several parts of the country posing a serious threat to crop loss and also to human health. With an objective to evaluate the genetic diversity of this snail, we have sampled this snail from different parts of India and analyzed its haplotype diversity by means of 16S rDNA sequence information. Apart from this, we have studied the phylogenetic relationships of the isolates sequenced in the present study in relation with other global populations by Bayesian and Maximum-likelihood approaches. Of the isolates sequenced, haplotype ‘C’ is the predominant one. A new haplotype ‘S’ from the state of Odisha was observed. The isolates sequenced in the present study clustered with its conspecifics from the Indian sub-continent. Haplotype network analyses were also carried out for studying the evolution of different haplotypes. It was observed that haplotype ‘S’ was associated with a Mauritius haplotype ‘H’, indicating the possibility of multiple introductions of A. fulica to India.

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References

  • Albuquerque FS, Peso-Aguiar MC, Assunção-Albuquerque MJT (2008) Distribution, feeding behaviour and control strategies of the exotic land snail Achatina fulica (Gastropoda: Pulmonata) in the northeast of Brazil. Braz J Biol 68(4):837–842

    Article  CAS  Google Scholar 

  • Ayyagari VS, Naravula J, Sreerama K (2017) Optimization of the isolation procedure of genomic DNA from a mucus laden pulmonate gastropod, Achatina fulica. Natl Acad Sci Lett 40:109–112. doi:10.1007/s40009-016-0535-0

    Article  CAS  Google Scholar 

  • Budha PB, Naggs F (2008) The giant African land snail Lissachatina fulica (Bowdich) in Nepal. Malacologist 50:19–21

    Google Scholar 

  • Civeyrel L, Simberloff D (1996) A tale of two snails: is the cure worse than the disease? Biodivers Conserv 5:1231–1252

    Article  Google Scholar 

  • Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9(10):1657–1660

    Article  CAS  Google Scholar 

  • Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9(8):772

    Article  CAS  Google Scholar 

  • Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucl Acids Res 32(5):1792–1797

    Article  CAS  Google Scholar 

  • Fernanda PO, Guimarães MCDA, Fernanda YT, Juliana ME (2010) Current distribution of Achatina fulica, in the state of São Paulo including records of Aelurostrongylus abstrusus (Nematoda) larvae infestation. Rev Inst Med Trop Sao Paulo 52(4):211–214

    Article  Google Scholar 

  • Fontanilla IKC, Maria IMPS, Garcia JRM, Ghate H, Naggs F et al (2014) Restricted genetic variation in populations of Achatina (Lissachatina) fulica outside of East Africa and the Indian Ocean islands points to the Indian Ocean islands as the earliest known common source. PLoS One 9(9):e105151. doi:10.1371/journal.pone.0105151

    Article  Google Scholar 

  • Huelsenbeck JP, Ronquist FR (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754–755

    Article  CAS  Google Scholar 

  • Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23(2):254–267

    Article  CAS  Google Scholar 

  • Jadhav AD, Dubal RS, Bagade RP, Sanadi RA, Kamble PL, Belgumpe S, Sathe TV (2016) Giant African snail, Achatina fulica Bowdich a destructive pest of V1 mulberry (Morus alba L.) by—a new report and control strategies from Kolhapur, Maharashtra, India. Biolife 4(1):184–188

    Google Scholar 

  • Jayashankar M, Murthy GSS, Krishnappa DK, Reddy MS (2014) Incidence of life stages of strongylid nematodes in the giant African snail, Achatina Fulica (Bowdich) in Bangalore region. Glob J Res Anal 3(4):193–194

    Article  Google Scholar 

  • Joseph EA (1966) The presence of Angiostrongylus Cantonensis in islands of the Indian ocean and probable role of the giant African snail, Achatina fulica in dispersal of the parasite to the Pacific islands. Can J Zool 44(6):1041–1049

    Article  Google Scholar 

  • Khan SA, Kumar S, Hussain MZ, Kalra N (2009) Climate change, climate variability and Indian agriculture: impacts vulnerability and adaptation strategies. In: Singh SN (ed) Climate change and crops, environmental science and engineering. Springer, Berlin, pp 19–38. doi:10.1007/978-3-540-88246-6_2

    Chapter  Google Scholar 

  • Lowe S, Browne M, Boudjelas S, De Poorter M (2000) 100 of the World’s worst invasive alien species: a selection from the global invasive species database. Published by the Invasive Species Specialist Group (ISSG) a specialist group of the Species Survival Commission (SSC) of the World Conservation Union (IUCN), 12 pp. First published as special lift-out in Aliens 12, December 2000. Updated and reprinted version: November 2004. Auckland, New Zealand

  • Maddison WP, Maddison DR (2015) Mesquite: a modular system for evolutionary analysis. Version 3.03. http://mesquiteproject.org

  • Mathai RS (2014) The snail spurt—an issue of concern. Int Res J Environ Sci 3(6):88–91

    Google Scholar 

  • Mead AR (1961) The giant African snail: a problem in economic malacology. The University of Chicago Press, USA

    Google Scholar 

  • Naggs Fred (1997) William Benson and the early study of land snails in British India and Ceylon. Arch Nat Hist 24(1):37–38

    Article  Google Scholar 

  • Nath Paras (2007) Emerging pest problems in India and critical issues in their management. In: Jain PC, Bhargava MC (eds) Entomology—novel approaches. New India Publishing Agency, New Delhi, pp 43–96

    Google Scholar 

  • Palumbi S, Martin A, Romano S, McMillan WO, Stice L, Gra-bowski G (2002) The simple fool’s guide to PCR. Version 2.0, University of Hawaii Press, Honolulu

  • Poucher C (1975) Eradication of the giant African snail in Florida. Annu Meet Fla State Hort Soc 88:523–524

    Google Scholar 

  • Ramakrishna Mitra SC, Dey A (2010) Annotated checklist of Indian land molluscs. Rec. Zool. Surv. India, Occasional paper no. 306, pp 1–359 (Published by the Director, Zoological Survey of India, Kolkata)

  • Rambaut A (2012) FigTree v1.4.2: tree figure drawing tool. Available from http://tree.bio.ed.ac.uk/software/figtree/

  • Rambaut A, Suchard MA, Xie W, Drummond AJ (2014) Tracer v1. 6. Available from http://beast.bio.ed.ac.uk/Tracer

  • Raut SK, Baker GM (2002) Achatina fulica Bowdich and other Achatinidae as pests in tropical agriculture. In: Baker GM (ed) Molluscs as crop pests. CAB International Publishing, Wallingford, pp 55–114

    Chapter  Google Scholar 

  • Raut SK, Ghose KC (1984) Pestiferous land snails of India. Tech Monogr Zool Surv India 11:1–151

    Google Scholar 

  • Rekha SR, Munsi M, Aravind NA (2015) Effect of climate change on invasion risk of giant African snail (Achatina fulica Ferussac, 1821: Achatinidae) in India. PLoS One 10(11):e0143724. doi:10.1371/journal.pone.0143724

    Article  Google Scholar 

  • Rudra NB, Robin G, Birinchi KB (2009) Snail: from present perspective to the history of Assam. Asian Agri-Hist 13(3):227–234

    Google Scholar 

  • Sheela T (1999) Management of giant African snail Achatina fulica Bowdich (Mollusca: Gastropoda) in North Bihar. Final project report submitted to Indian Council of Agricultural Research, New Delhi, India

  • Silvana CT, Faraco FA, Norma CS, Robert HC, Monica AF (2007) Rapid spread of an invasive snail in South America: the giant African snail, Achatina fulica, in Brazil. Biol Invasions 9:693–702. doi:10.1007/s10530-006-9069-6

    Article  Google Scholar 

  • Sokolov EP (2000) An improved method for DNA isolation from mucopolysaccharide-rich molluscan tissues. J Moll Stud 66:573–575

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

  • Vanitha K, Karuppuchamy P, Sivasubramanian P (2011) Record of gastropod pests on vanilla and their population dynamics in relation to weather parameters. Pest Manag Horticult Ecosyst 17(1):56–59

    Google Scholar 

  • Wade CM, Mordan PB (2000) Evolution within the gastropod molluscs: using the ribosomal RNA gene-cluster as an indicator of phylogenetic relationships. J Moll Stud 66:565–570

    Article  Google Scholar 

Download references

Acknowledgements

This work has been funded by the Science and Engineering Research Board, Department of Science and Technology, Government of India (Sanction order: SB/SO/AS-138/2012 dated 30-10-2013). Authors gratefully acknowledge the experts from Malacological section, Zoological Survey of India, Kolkata for identifying the A. fulica specimen.

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  1. Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research University, Vadlamudi, Andhra Pradesh, 522213, India

    Vijaya Sai Ayyagari & Krupanidhi Sreerama

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  1. Vijaya Sai Ayyagari
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  2. Krupanidhi Sreerama
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Correspondence to Vijaya Sai Ayyagari.

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Ayyagari, V., Sreerama, K. Evaluation of haplotype diversity of Achatina fulica (Lissachatina) [Bowdich] from Indian sub-continent by means of 16S rDNA sequence and its phylogenetic relationships with other global populations. 3 Biotech 7, 252 (2017). https://doi.org/10.1007/s13205-017-0877-4

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