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Degradation and conversion of endosulfan by newly isolated Pseudomonas mendocina ZAM1 strain

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Abstract

Endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. Therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. A novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as ZAM1 strain. The ZAM1 bacterial strain was further identified as Pseudomonas mendocina based on the biochemical and molecular analysis. 16sRNA sequence analysis of ZAM1 strain shows maximum similarity with known endosulfan-degrading bacteria (Pseudomonas putida), respectively. Enrichment was carried out using the endosulfan as sole sulfur source. The ZAM1 strain was able to use α and β endosulfan as a sole sulfur source. Our results showed that ZAM1 strain degrades endosulfan >64.5% (50 mg/l) after 12 days of incubation. The residues were analyzed by GC–MS analysis and confirmed the formation of metabolites of dieldrin, 2 heptanone, methyl propionate, and endosulfan lactone compounds. Hence, these results indicate that the ZAM1 strain is a promising bacterial source for detoxification of endosulfan residues in the environment.

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Acknowledgements

Authors are thankful to Mohammed Sathak College of Arts & Science (University of Madras), Chennai, for providing the lab facilities to carry out this work.

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Authors and Affiliations

  1. MSCAS College of Arts and Science, Chennai, Tamil Nadu, India

    Zahoor A. Mir

  2. National Research Centre on Plant Biotechnology, New Delhi, India

    Sajad Ali & Anshika Tyagi

  3. St Xavier’s College, Tirunelveli, Tamil Nadu, India

    Ajaz Ali

  4. Department of Genetics, IARI, PUSA Campus, New Delhi, India

    Javaid A. Bhat

  5. Amity Institute of Biotechnology, Amity University, Noida, India

    Praful Jaiswal

  6. Center of Excellence in Environmental Studies, King Abdul-Aziz University, Jeddah, 21589, Kingdom of Saudi Arabia

    Huda A. Qari & Mohammad Oves

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  1. Zahoor A. Mir
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  4. Ajaz Ali
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Contributions

ZAM carried out experimental work; SA, AT, and AA helped in statistical analysis and drafting the manuscript; HAQ and JAB helped in manuscript editing; MO has designed the work and approved the final manuscript.

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Correspondence to Mohammad Oves.

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Mir, Z.A., Ali, S., Tyagi, A. et al. Degradation and conversion of endosulfan by newly isolated Pseudomonas mendocina ZAM1 strain. 3 Biotech 7, 211 (2017). https://doi.org/10.1007/s13205-017-0823-5

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