Skip to main content
Log in

Pectin from muskmelon (Cucumis melo var. reticulatus) peels: extraction optimization and physicochemical properties

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Pectin derived from plant waste sources is currently focused as an economical and eco-friendly approach. Optimization of pectin extraction from muskmelon peel by response surface methodology (RSM) was investigated in this study. Box–Behnken Design (BBD) was used to identify the optimal level of the extraction variables such as time, pH and temperature. A second-order model equation for pectin extraction was obtained from multiple regression analysis of experimental data with the correlation coefficient (R 2) value of 0.92. ANOVA results showed that linear effect of temperature and combined effect of pH with temperature were found significant for pectin extraction from muskmelon peel. Validation results had good agreement with the predicted results. Pectin extracted from muskmelon peel was classed as high methoxy pectin with the equivalent weight of 384.5 g/mol. Non-newtonian pseudoplastic flow behaviour was observed for muskmelon pectin from the viscosity studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Alba K, Laws AP, Kontogiorgos V (2015) Isolation and characterization of acetylated LM-pectins extracted from okra pods. Food Hydrocoll 43:726–735

    Article  CAS  Google Scholar 

  • Albishri HM, Almaghrabi OA, Moussa TAA (2013) Characterization and chemical composition of fatty acids content of watermelon and muskmelon cultivars in Saudi Arabia using gas chromatography/mass spectroscopy. Pharmacogn Mag 9:58–66

    Article  CAS  Google Scholar 

  • Casas-Orozco D, Villa AL, Bustamante F, González LM (2015) Process development and simulation of pectin extraction from orange peels. Food Bioprod Process 96:86–98

    Article  CAS  Google Scholar 

  • Castillo-Israel KAT, Baguio SF, Diasanta MDB, Lizardo RCM, Dizon EI, Mejico MIF (2015) Extraction and characterization of pectin from Saba banana [Musa ‘saba’(Musa acuminata x Musa balbisiana)] peel wastes: a preliminary study. Int Food Res J 22:202–207

    CAS  Google Scholar 

  • Chen Y, Zhang JG, Sun HJ, Wei ZJ (2014) Pectin from Abelmoschus esculentus optimization of extraction and rheological properties. Int J Biol Macromol 70:498–505

    Article  CAS  Google Scholar 

  • de Oliveira CF, Giordani D, Lutckemier R, Gurak PD, Cladera-Olivera F, Marczak LDF (2016) Extraction of pectin from passion fruit peel assisted by ultrasound. LWT-Food Sci Technol 71:110–115

    Article  Google Scholar 

  • Denman LJ, Morris GA (2015) An experimental design approach to the chemical characterisation of pectin polysaccharides extracted from Cucumis melo Inodorus. Carbohydr Polym 117:364–369

    Article  CAS  Google Scholar 

  • Doran MP (2013) Bioprocess engineering principles, 2nd edn. Elsevier, Amsterdam

    Google Scholar 

  • Hosseini SS, Khodaiyan F, Yarmand MS (2016a) Optimization of microwave assisted extraction of pectin from sour orange peel and its physicochemical properties. Carbohydr Polym 140:59–65

    Article  CAS  Google Scholar 

  • Hosseini SS, Khodaiyan F, Yarmand MS (2016b) Aqueous extraction of pectin from sour orange peel and its preliminary physicochemical properties. Int J Biol Macromol 82:920–926

    Article  CAS  Google Scholar 

  • Joye DD, Luzio GA (2000) Process for selective extraction of pectins from plant material by different pH. Carbohydr Polym 43:337–342

    Article  CAS  Google Scholar 

  • Korish M (2015a) Potential utilization of Citrullus lanatus var. Colocynthoides waste as a novel source of pectin. J Food Sci Technol 52:2401–2407

    Article  CAS  Google Scholar 

  • Korish M (2015b) Faba bean hulls as a potential source of pectin. J Food Sci Technol 52:6061–6066

    Article  CAS  Google Scholar 

  • Kratchanova M, Pavlova E, Panchev I (2004) The effect of microwave heating of fresh orange peels on the fruit tissue and quality of extracted pectin. Carbohydr Polym 56:181–185

    Article  CAS  Google Scholar 

  • Kulkarni SG, Vijayanand P (2010) Effect of extraction conditions on the quality characteristics of pectin from passion fruit peel (Passiflora edulis f. Flavicarpa L.). LWT-Food Sci Technol 43:1026–1031

    Article  CAS  Google Scholar 

  • Li DQ, Du GM, Jing WW, Li JF, Yan JY, Liu ZY (2015) Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid. Carbohydr Polym 129:108–114

    Article  CAS  Google Scholar 

  • Liew SQ, Chin NL, Yusof YA (2014) Extraction and characterization of pectin from passion fruit peels. Agric Agric Sci Procedia 2:231–236

    Article  Google Scholar 

  • Liew SQ, Chin NL, Yusof YA, Cheok CY (2015) Citric acid extraction of pectin from tropical fruit peels of passion fruit, dragon fruit and soursop. J Food Agric Environ 13:45–51

    CAS  Google Scholar 

  • Maran JP (2015) Statistical optimization of aqueous extraction of pectin from waste durian rinds. Int J Biol Macromol 73:92–98

    Article  CAS  Google Scholar 

  • Minjares-Fuentesa R, Femeniaa A, Garaua MC, Meza-Velázquezb JA, Simala S, Rossell C (2014) Ultrasound-assisted extraction of pectins from grape pomace using citric acid: a response surface methodology approach. Carbohydr Polym 106:179–189

    Article  Google Scholar 

  • Oliveira TÍS, Rosa MF, Cavalcante FL, Pereira PHF, Moates GK, Wellner N, Mazzetto SE, Waldron KW, Azeredo HMC (2015) Optimization of pectin extraction from banana peels with citric acid by using response surface methodology. Food Chem 198:113–118

    Article  Google Scholar 

  • Owens HS, McCready RM, Shepard AD, Schultz TH, Pippen EL, Swenson HA, Miers JC, Erlandsen RF, Maclay WD (1952) Methods used at western regional research laboratory for extraction of pectic materials. USDA Bur Agric Ind Chem pp 9

  • Padmanaban S, Balaji N, Muthukumaran C, Tamilarasan K (2015) Statistical optimization of process parameters for exopolysaccharide production by Aureobasidium pullulans using sweet potato based medium. 3 Biotech 5:1067–1073

    Article  Google Scholar 

  • Pandit SG, Vijayanand P, Kulkarni SG (2015) Pectic principles of mango peel from mango processing waste as influenced by microwave energy. LWT-Food Sci Technol 64:1010–1014

    Article  CAS  Google Scholar 

  • Qiu L-P, Zhao G-L, Wu H, Jiang L, Li X-F, Liu J-J (2010) Investigation of combined effects of independent variables on extraction of pectin from banana peel using response surface methodology. Carbohydr Polym 80:326–331

    Article  CAS  Google Scholar 

  • Sadasivam S, Manickam A (2008) Biochemical methods, 3rd edn. New Age International (P) Limited, India

    Google Scholar 

  • Salunkhe DK, Kadam SS (1998) Handbook of vegetable science and technology: production, composition, storage and processing. Marcel-Dekker, New York

    Google Scholar 

  • Seixas FL, Fukuda DL, Turbiani FRB, Garcia PS, Petkowicz CLO, Jagadevan S, Gimenes ML (2014) Extraction of pectin from passion fruit peel (Passiflora edulis f. flavicarpa) by microwave-induced heating. Food Hydrocoll 38:186–192

    Article  CAS  Google Scholar 

  • Seslija S, Dj Veljovic, Kalagasidis KM, Stevanovic J, Velickovic S, Popovic I (2016) Cross-linking of highly methoxylated pectin with copper: the specific anion influence. New J Chem 40:1618–1625

    Article  CAS  Google Scholar 

  • Sharma PC, Gupta A, Kaushal P (2014) Optimization of method for extraction of pectin from apple pomace. Indian J Nat Prod Resour 5:184–189

    CAS  Google Scholar 

  • Sharmila G, Nikitha VS, Ilaiyarasi S, Dhivya K, Rajasekar V, Manojkumar N, Muthukumaran K, Muthukumaran C (2016) Ultrasound assisted extraction of total phenolics from Cassia auriculata leaves and evaluation of its antioxidant activities. Ind Crops Prod 84:13–21

    Article  CAS  Google Scholar 

  • Singthong J, Cui SW, Ningsanond S, Goff HD (2004) Structural characterization, degree of esterification and some gelling properties of Krueo Ma Noy (Cissampelos pareira) pectin. Carbohydr Polym 58:391–400

    Article  CAS  Google Scholar 

  • Srikanth S, Swathi M, Tejaswini M, Sharmila G, Muthukumaran C, Jaganathan MK, Tamilarasan K (2014) Statistical optimization of molasses based exopolysaccharide and biomass production by Aureobasidium pullulans MTCC 2195. Biocatal Agric Biotechnol 3:7–12

    Google Scholar 

  • Tang PY, Kek TS, Gan CZ, Hee CY, Chong CH, Woo KK (2011) Yield and Some Chemical Properties of Pectin Extracted from the Peels of Dragon Fruit [Hylocereus polyrhizus (Weber) Britton and Rose]. Philipp Agric Sci 94:307–311

    CAS  Google Scholar 

  • Thirugnanasambandham K, Sivakumar V, Maran JP (2014) Process optimization and analysis of microwave assisted extraction of pectin from dragon fruit peel. Carbohydr Polym 112:622–626

    Article  CAS  Google Scholar 

  • Vriesmann LC, Teofalo RF, Petkowicz O (2012) Extraction and characterization of pectin from cacao pod husks (Theobroma cacao L.)with citric acid. LWT-Food Sci Technol 49:108–116

    Article  CAS  Google Scholar 

  • Wang X, Chen Q, Lu X (2014) Pectin extracted from apple pomace and citrus peel by subcritical water. Food Hydrocoll 38:129–137

    Article  CAS  Google Scholar 

  • Wang W, Ma X, Jiang P, Hu L, Zhi Z, Chen J, Ding T, Ye X, Liu D (2016) Characterization of pectin from grapefruit peel: a comparison of ultrasound-assisted and conventional heating extractions. Food Hydrocoll 61:730–739

    Article  CAS  Google Scholar 

  • Wikiera A, Mika M, Starzynska-Janiszewska A, Stodolak B (2016) Endo-xylanase and endo-cellulase-assisted extraction of pectin from apple pomace. Carbohydr Polym 142:199–205

    Article  CAS  Google Scholar 

  • Yeoh S, Shi J, Langrish T (2008) Comparison between different techniques for water based extraction of pectins from orange peels. Chem Eng Commun 19:511–520

    Article  Google Scholar 

  • Zhang W, Xu P, Zhang H (2015) Pectin in cancer therapy: a review. Trend Food Sci Technol 44:258–271

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are thankful to The Principal and The Head, Department of Industrial Biotechnology, Government College of Technology, Coimbatore for their encouragement and providing facilities to carry out this study.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chandrasekaran Muthukumaran.

Ethics declarations

Conflict of interest

The authors declared that there is no conflict of interest on publication of this article.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Muthukumaran, C., Banupriya, L., Harinee, S. et al. Pectin from muskmelon (Cucumis melo var. reticulatus) peels: extraction optimization and physicochemical properties. 3 Biotech 7, 66 (2017). https://doi.org/10.1007/s13205-017-0655-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-017-0655-3

Keywords

Navigation