Abstract
We studied the microbial communities associated with the Baikal ostracods, Candona sp. (Candonidae). Bacterial biofilms were identified on the shell valves of the ostracods localized near epibiotic ciliates of the genus Lagenophrys (Ciliophora: Lagenoprydae). In situ hybridization confirmed the presence of eubacterial cells in different parts of the ostracod’s body. Bacteria of two morphotypes were detected in bacterial biofilms, including filamentous bacteria. Next-generation sequencing of 16S rRNA bacterial gene fragment allowed us to identify representatives of five bacterial phyla. Members of the phyla Proteobacteria and Bacteroidetes typical of non-marine epibiotic associations of ostracods were dominant taxa in the ostracod microbiomes. Members of other taxa (Actinobacteria, Gracilibacteria and Cyanobacteria) are likely facultative and consumed by ostracods together with detritus. In the ostracod microbiome, a significant proportion (20% of all reads) belonged to the sequences of the genus Rickettsia related to intracellular parasites.
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Acknowledgements
The authors thank the crew of the research vessel “G.Yu. Vereschagin” for help in sampling. This study was carried out using computational resources of the HPC-cluster “Akademik V.M. Matrosov” of Irkutsk Supercomputer Center of SB RAS.
Microscopic studies were carried out in the Electron Microscopy Center of Collective Instrumental Center “Ultramicroanalysis” at Limnological Institute Siberian Branch of the Russian Academy of Sciences (http://www.lin.irk.ru/).
This work was financially supported by the State Task, project No. 0279-2021-0006 (LIN SB RAS).
We also acknowledge the valuable comments and suggestions of two anonymous reviewers.
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TZ conceived, designed and coordinated the study. Sample collection, material preparation and biomolecular experiments were performed by IK and TS. NGS data analysis was performed by SB, IK and YG. SB, SC and AZ prepared figures and tables. The first draft of the manuscript was written by TZ and IK; all authors commented on the previous versions of the manuscript. All authors have read and approved the final manuscript.
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Khalzov, I.A., Bukin, S.V., Zakharenko, A.S. et al. Microbial communities associated with the ostracods Candona sp. inhabiting the area of the methane seep Goloustnoye (Lake Baikal). Symbiosis 85, 163–174 (2021). https://doi.org/10.1007/s13199-021-00802-3
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DOI: https://doi.org/10.1007/s13199-021-00802-3