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Two endosymbiotic bacteria, Wolbachia and Arsenophonus, in the brown planthopper Nilaparvata lugens

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Abstract

The brown planthopper Nilaparvata lugens harbors intracellular fungal yeast-like symbionts and endosymbiotic bacteria, with the latter mainly comprising Wolbachia and Arsenophonus. In this study, Wolbachia or Arsenophonus were detected in all 15 brown planthopper populations collected from China and Southeastern Asian countries. Furthermore, Polymerase Chain Reaction (PCR) analysis of the individuals in a population that was infected by both Wolbachia and Arsenophonus showed that each individual was infected by only one of the two symbiotic bacteria. Real-time quantitative PCR showed that both endosymbionts are mainly localized in the mycetocytes of the fat body. Reciprocal crosses between the Wolbachia + and Arsenophonus + brown planthopper populations showed that both bacteria were maternally transmitted. Our results showed that the brown planthopper populations are extensively infected by Wolbachia or Arsenophonus, and the two bacteria may be exclusive in each host individual. This finding might be helpful for further studies on the biological functions of the endosymbiotic bacteria and will deepen our understanding of the complicated symbiosis system in this host.

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Acknowledgments

We thank Professor Ming-Xing Jiang, Professor Zeng-Rong Zhu, Professor Qi-Yi Tang, Dr. Zhan-Yu Liu of Zhejiang University, Professor Jin-Cai Wu of Yangzhou University, Dr. Hong-Xing Xu of Zhejiang Academy of Agricultural Sciences and Dr. Qiang Zhou of Sun- Yat Sun University for their kind helps in collecting brown planthopper samples. This work was supported by National Basic Research Program of China (973 Program, No. 2010CB126200) and the National Natural Science Foundation of China (Grant No. 31272374 and 31070136).

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Authors and Affiliations

  1. Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China

    Lv-Yu Qu, Yi-Han Lou, Hai-Wei Fan, Yu-Xuan Ye, Hai-Jian Huang, Meng-Qi Hu, Ya-Ni Zhu & Chuan-Xi Zhang

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  1. Lv-Yu Qu
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  2. Yi-Han Lou
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Correspondence to Chuan-Xi Zhang.

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Lv-Yu Qu and Yi-Han Lou contributed equally to this work.

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Qu, LY., Lou, YH., Fan, HW. et al. Two endosymbiotic bacteria, Wolbachia and Arsenophonus, in the brown planthopper Nilaparvata lugens . Symbiosis 61, 47–53 (2013). https://doi.org/10.1007/s13199-013-0256-9

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