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Effect of Piriformospora indica and Sebacina vermifera on plant growth and essential oil yield in Thymus vulgaris in vitro and in vivo experiments

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Abstract

Thymus vulgaris of family Lamiaceae is one of the most plants in pharmacy industries. In this study effect of Piriformospora indica and Sebacina vermifera on growth and development plant, yield and composition of the essential oil in a completely randomized design were evaluated in vitro and in pot culture experiments. Plants were studied by means of plant height, shoot fresh and dry weights, number of shoots, root length, root fresh and dry weights and essential oil analyses. The oil was extracted from the dry matter of shoots by hydro distillation, and their composition was determined by GC/MS. In vitro and in vivo cultures showed that plant height and root length increased in pots inoculated with S. vermifera and P. indica. Maximum fresh and dry weight (shoot and root), number of shoots were observed in pots inoculated with P. indica. In thyme inoculated with S. vermifera and P. indica oil yield increased as compared to non-inoculated control plants. GC and GC/MS revealed that the level of thymol was enhanced as the effect of S. vermifera and P. indica.

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Acknowledgment

This study was supported by the Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University. We thank the Research Institute of Forests and Rangelands, Tehran, Iran for GC and GC-mass analyses.

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Authors and Affiliations

  1. Department of Plant Pathology, School of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran

    Hossein Kari Dolatabadi, Ebrahim Mohammadi Goltapeh & Babak Pakdaman Sardrood

  2. Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran

    Ahmad Moieni

  3. Phytochemistry Group, Department of Medicinal plants & By-products, Research Institute of Forest and Ranglands, Tehran, Iran

    Kamkar Jaimand

  4. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Sector 125, Noida, 201303, UP, India

    Ajit Varma

Authors
  1. Hossein Kari Dolatabadi
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  2. Ebrahim Mohammadi Goltapeh
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  3. Ahmad Moieni
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  4. Kamkar Jaimand
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  5. Babak Pakdaman Sardrood
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  6. Ajit Varma
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Correspondence to Hossein Kari Dolatabadi or Ebrahim Mohammadi Goltapeh.

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Dolatabadi, H.K., Goltapeh, E.M., Moieni, A. et al. Effect of Piriformospora indica and Sebacina vermifera on plant growth and essential oil yield in Thymus vulgaris in vitro and in vivo experiments. Symbiosis 53, 29–35 (2011). https://doi.org/10.1007/s13199-010-0104-0

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  • DOI: https://doi.org/10.1007/s13199-010-0104-0

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