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Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi

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Abstract

In this study, the production of extracellular enzymes by fungi associated with southern pine beetle was investigated for the first time. Cellulase and polyphenol oxidase production were analyzed for three beetle associated fungi. Only the mutualistic symbiont Entomocorticium sp. A was found to produce cellulases and polyphenol oxidase. In time course analyses of cellulase production in batch cultures, Entomocorticium sp. A showed maximum activity of 0.109 U/ml and 0.141 U/ml for total cellulase and endoglucanase activity respectively. Polyphenol oxidase production was simultaneous with fungal growth. Characterization of polyphenol oxidase by activity staining suggests that the enzyme is a tyrosinase/catechol oxidase. Enzyme assays in the presence of polyphenol oxidase inhibitors support the results of the activity staining.

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References

  • Ayres MP, Wilkens RT, Ruel JJ, Vallery E (2000) Fungal relationships and the nitrogen budget of phloem-feeding bark beetles (Coleoptera:Scolytidae). Ecology 81:2198–2210.

    Article  Google Scholar 

  • Barras SJ (1970) Antagonism between Dendroctonus frontalis and the fungus Ceratocystis minor. Annals of the Entomological Society of America 63:1187–1190.

    Google Scholar 

  • Bavendamm W (1928) Über das Vorkommen und den Nachweis von Oxydasen bei holzzerstörenden Pilzen. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 38:257–276.

    CAS  Google Scholar 

  • Bramble WC, Holst EC (1940) Fungi associated with Dendroctonus frontalis in killing shortleaf pines and their effect on conduction. Phytopathology 30:881–899.

    CAS  Google Scholar 

  • Coppedge BR, Stephen FM, Felton GW (1995) Variation in female southern pine beetle size and lipid content in relation to fungal associates. Canadian Entomologist 127:145–154.

    Google Scholar 

  • Delalibera I, Handelsman J, Raffa KF (2005) Contrasts in cellulolytic activities of gut microorganisms between the wood borer, Saperda vestita (Coleoptera: Cerambycidae), and the bark beetles, Ips pini and Dendroctonus frontalis (Coleoptera: Curculionidae). Environmental Entomology 34:541–547.

    Article  Google Scholar 

  • Hsiau PTW, Harrington TC (2003) Phylogenetics and adaptations of basidio-mycetous fungi fed upon by bark beetles (Coleoptera: Scolytidae). Symbiosis 34:111–131.

    Google Scholar 

  • Jacobs K, Kirisits T (2003) Ophiostoma kryptum sp. nov. from Larix decidua and Picea abies in Europe, similar to O. minus. Mycological Research 107:231–1242.

    Article  CAS  Google Scholar 

  • Kinzer GW, Fentiman AF, Page TF, Foltz RL, Vite JP, Pitman GB (1969) Bark beetle attractants: Identification, synthesis and field bioassay of a new compound isolated from Dendroctonus. Nature 221:447–478.

    Article  Google Scholar 

  • Klepzig KD (2006) Melanin and the southern pine beetle-fungus symbiosis. Symbiosis 40:137–140.

    Google Scholar 

  • Klepzig KD, Six DL (2004) Bark beetle fungal symbiosis: Context dependency in complex interactions. Symbiosis 37:189–206.

    Google Scholar 

  • Klepzig KD, Wilkens RT (1997) Competitive interactions among symbiotic fungi of the southern pine beetle. Applied and Environmental Microbiology 63:621–627.

    CAS  PubMed  Google Scholar 

  • Klepzig KD, Robison DJ, Fowler G, Minchin PR, Hain FP, Allen HL (2005) Effects of mass inoculation on induced oleoresin response in intensively managed loblolly pine. Tree Physiology 25:681–688.

    PubMed  Google Scholar 

  • Martins LF, Kolling D, Camassola M, Dillon AJ, Ramos LP (2008) Comparison of Penicillium echinulatum and Trichoderma reesei cellulases in relation to their activity against various cellulosic substrates. Bioresource Technology 99:1417–1424.

    Article  CAS  PubMed  Google Scholar 

  • Miranda M, Zarivi O, Bonfigli A, Amicarelli F, Aimola P, Ragnelli AM (1997) Melanogenesis, tyrosinase expression, and reproductive differentiation in black and white truffles (ascomycotina). Pigment Cell Research 10:46–53.

    Article  CAS  PubMed  Google Scholar 

  • Ogel ZB, Yuzugullu Y, Mete S, Bakir U, Kaptan Y, Sutay D (2006) Production, properties and application to biocatalysis of a novel extracellular alkaline phenol oxidase from the thermophilic fungus Scytalidium thermophilum. Applied Microbiology and Biotechnology 71:853–862.

    Article  CAS  PubMed  Google Scholar 

  • Paine TD, Raffa KF, Harrington TC (1997) Interactions among Scolytid bark beetles, their associated fungi, and live host conifers. Annual Review of Entomology 42:179–206.

    Article  CAS  PubMed  Google Scholar 

  • Payne, T.L. 1983. Behavior. In: History, Status and Future Needs for Entomology Research in Southern Forests: Proceedings of the 10th Anniversary of the East Texas Forest Entomology Seminar. Texas Agricultural Experiment Station, Texas A&M University System, College Station, TX, USA. 72 pp.

  • Przybyl K, Dahm H, Ciesielska A, Moliński K (2006) Cellulolytic activity and virulence of Ophiostoma ulmi and O. novo-ulmi isolates. Forest Pathology 36:58–67.

    Article  Google Scholar 

  • Raffa KF, Phillips TW, Salom SM (1993) Strategies and mechanisms of host colonization by bark beetles. In: Schowalter T, Filip G (eds) Beetle-Pathogen Interactions in Conifer Forests. Academic Press, San Diego, USA, pp 102–128.

    Google Scholar 

  • Rescigno A, Sollai F, Rinaldi AC, Soddu G, Sanjust E (1997) Polyphenol oxidase activity staining in polyacrylamide electrophoresis gels. Journal of Biochemical and Biophysical Methods 34:155–159.

    Article  CAS  PubMed  Google Scholar 

  • Rescigno A, Sollai F, Pisu B, Rinaldi A, Sanjust E (2002) Tyrosinase inhibition: general and applied aspects. Journal of Enzyme Inhibition and Medicinal Chemistry 17:207–218.

    Article  CAS  PubMed  Google Scholar 

  • Rösch R, Liese W, Berndt H (1969) Studies on the enzymes of blue-stain flungi. I. Cellulase, polygalacturonase, pectinesterase and laccase activity. Archives of Microbiology 67:28–50.

    Google Scholar 

  • Ruijssenaars J, Hartsmans S (2000) Plate screening methods for the detection of polysaccharase producing microorganisms. Applied Microbiology and Biotechnology 55:143–149.

    Article  Google Scholar 

  • Sánchez MM, Irwin D, Pastor FIJ, Wilson DB, Diaz P (2004) Synergistic activity of Paenibacillus sp. BP-23 cellobiohydrolase Cel48C in association with the contiguous endoglucanase Cel9B and with endo- or exo-acting glucanases from Thermobifida fusca. Biotechnology and Bioengineering 87:161–169.

    Article  PubMed  CAS  Google Scholar 

  • Score JA, Palfreyman JW, White NA (1997) Extracellular phenoloxidase and peroxidase enzyme production during interspecific fungal interactions. International Biodeterioration and Biodegradation 39:225–233.

    Article  CAS  Google Scholar 

  • Thatcher, R.C. 1960. Bark beetles affecting southern pines: Review of current knowledge. USDA Forest Service Occasional Paper 180. Pineville, LA, USA.

  • Thatcher, R.C., Searcy, J.L., Coster, J.E., and Hertel, G.D. 1980. The southern pine beetle. USDA Forest Service Science Education Administration Technical Bulletin No. 1631. Pineville, LA, USA.

  • Tomme P, Warren RAJ, Gilkes NR (1995) Cellulose hydrolysis by bacteria and fungi. Advances in Microbial Physiology 37:1–81

    Article  CAS  PubMed  Google Scholar 

  • Walker JRL, McCallion RF (1980) The selective inhibition of ortho- and para-diphenol oxidases. Phytochemistry 19:373–377.

    Article  CAS  Google Scholar 

  • Wood, T.M. and Bhat, K.M. 1988. Methods for measuring cellulase activities. In: Methods in Enzymology. Academic Press, San Diego, USA, 160, ch 9.

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Authors and Affiliations

  1. Department of Biotechnology, Middle East Technical University, 06531, Ankara, Turkey

    Abduvali Valiev & Zumrut B. Ogel

  2. Department of Food Engineering, Middle East Technical University, 06531, Ankara, Turkey

    Zumrut B. Ogel

  3. Southern Research Station, USDA Forest Service, Asheville, NC, 28804, USA

    Kier D. Klepzig

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  1. Abduvali Valiev
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  2. Zumrut B. Ogel
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  3. Kier D. Klepzig
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Correspondence to Zumrut B. Ogel.

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Valiev, A., Ogel, Z.B. & Klepzig, K.D. Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi. Symbiosis 49, 37–42 (2009). https://doi.org/10.1007/s13199-009-0007-0

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  • DOI: https://doi.org/10.1007/s13199-009-0007-0

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