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Antibacterial and antiparasitic activities analysis of a hepcidin-like antimicrobial peptide from Larimichthys crocea

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Abstract

As an economically important marine fish, the large yellow croaker Larimichthys crocea suffered from marine white spot disease caused by the ectoparasite Cryptocaryon irritans in recent years. This disease not only could result in physiological damage, but also lead to secondary bacterial invasion. Reports indicated some AMPs (antimicrobial peptides) were of antiparasitic activity to C. irritans. Hepcidin-like (Lc-HepL) was one of the significant differential expression genes excavated from the transcriptome following a challenge with C. irritans. In this study, we characterized this AMP’s bioactivity based on the levels of mRNA and protein. After challenged by C. irritans, qRT-PCR showed Lc-HepL was significantly upregulated in six tissues, including gill, muscle, liver, head kidney and spleen during theront infection, trophont falling off, and secondary bacterial invasion stages, which implicated a role Lc-HepL played in the immune defense against C. irritans and secondary bacterial infection. Recombinant Lc-HepL (rLc-HepL) was induced and purified successfully. rLc-HepL exhibited antibacterial activity to certain bacteria in a dose- and time-dependent manners. Anti-C. irritans activity was explored for the first time and found it could cause the theronts membrane rupture and contents leakage. These results provided the first evidence that Lc-HepL had strong antiparasitic activity against marine fish ectoparasites C. irritans theronts. Together, data indicated that Lc-HepL might be an important component in the innate immune system against C. irritans and has the potential to be employed in future drug development.

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Authors and Affiliations

  1. State Key Laboratory of Large Yellow Croaker Breeding, Ningde, 352103, China

    Libing Zheng, Jun Wang, Ying Pan, Jia Chen & Weiqiang Zheng

  2. Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China

    Yuan Li & Longshan Lin

  3. National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, 316022, China

    Libing Zheng

Authors
  1. Libing Zheng
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  2. Yuan Li
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  3. Jun Wang
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  4. Ying Pan
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  5. Jia Chen
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  6. Weiqiang Zheng
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  7. Longshan Lin
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Correspondence to Longshan Lin.

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The National Key Research and Development Program of China under contract No. 2018YFC1406302; the Local Science and Technology Development Project Guide by the Central Government under contract No. 2017L3019; the Development Project Guide by the Central Government under contract No. 2017L3019; the Technical Innovation Platform for Large Yellow Croaker under contract No. XDHT2018143A; the Major Special Projects of Fujian Province under contract No. 2016NZ0001.

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Zheng, L., Li, Y., Wang, J. et al. Antibacterial and antiparasitic activities analysis of a hepcidin-like antimicrobial peptide from Larimichthys crocea. Acta Oceanol. Sin. 39, 129–139 (2020). https://doi.org/10.1007/s13131-020-1580-6

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  • DOI: https://doi.org/10.1007/s13131-020-1580-6

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