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Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China

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Abstract

A great deal of oil contaminated the shoreline by the Qingdao oil pipeline explosion in 2013. The four oil-degrading consortia were enriched from sediment samples with crude oil as sole carbon and energy sources. The biodiversity and community analysis showed that the Luteibacter, Parvibaculum and a genus belonging to Alcanivoracaceae were found predominant bacteria in the four consortia, which belonged to Proteobacteria. Nine strains exhibiting distinct 16S rRNA gene sequences were isolated from the consortia. These strains were identified to eight genera based on 16S rRNA gene sequences. Five of the nine strains degraded more than 30% of the crude oil in two weeks by gravimetric method. From the analysis of GC-MS, most of the isolated strains tended to degrade n-alkanes rather than PAHs. Five strains showed high degrading ability of the total n-alkanes. Only Strain D2 showed great PAHs degrading ability and the degrading rates ranged from 34.9% to 77.5%. The sequencing analysis of the oil-degrading consortia confirmed that the genus of Alcanivorax was one of the dominant bacteria in Consortia A and E and Strain E4 might be one of the dominant bacteria. The strains obtained in this study demonstrated the potential for oil bioremediation in oil-contaminated beach ecosystems.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Wei Gao

  2. Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China

    Wei Gao, Xiangxing Gao, Bin Han, Yiran Zhang, Xinzi Li, Xiaofei Yin, Chengjun Sun, Qian Li, Zhisong Cui, Xiao Luan & Li Zheng

  3. National Deep Sea Center, Qingdao, 266237, China

    Xiangxing Gao

  4. College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China

    Tiezhu Mi

  5. Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao, 266100, China

    Tiezhu Mi & Zhigang Yu

  6. Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China

    Tiezhu Mi, Zhigang Yu & Li Zheng

Authors
  1. Wei Gao
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  2. Xiangxing Gao
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  3. Tiezhu Mi
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  4. Bin Han
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  7. Xiaofei Yin
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  8. Chengjun Sun
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  9. Qian Li
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  10. Zhisong Cui
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  11. Xiao Luan
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  12. Zhigang Yu
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  13. Li Zheng
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Corresponding author

Correspondence to Li Zheng.

Additional information

Foundation item: The National Natural Science Foundation of China under contract Nos 41406127 and 41476103; the Basic Scientific Fund for National Public Research Institutes of China under contract No. 2015T05; the National Natural Science Foundation of China-Shandong Joint Funded Project under contract No. U1406403; the Marine Science and Technology Project of Huangdao District under contract No. 2014-4-20; the 2012 Taishan Scholar Award and China-ASEAN Maritime Cooperation Fund East Asia Marine Cooperation Platform.

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Gao, W., Gao, X., Mi, T. et al. Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China. Acta Oceanol. Sin. 38, 54–64 (2019). https://doi.org/10.1007/s13131-019-1353-2

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  • DOI: https://doi.org/10.1007/s13131-019-1353-2

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