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Effects of UV-B radiation and different light repair conditions on the early development of the tetraspores of Chondrus ocellatus Holm

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Abstract

The effects of exposure to UV-B radiation, 280–315 nm, and dark or dark repair conditions on the early development of Chondrus ocellatus Holm collected from Qingdao coast were studied under the laboratory condition. After being exposed to different doses [0, 36, 72, 108, 144 and 180 J/(m2·d)] of UV-B radiation, one treatment of tetraspores were put back to normal culture condition (PAR), the other treatment were put into dark condition to repair for 2 h. During the cultivation, the diameter of the tetraspores were recorded every 4 d till the 50th day when vertical branches formed in all treatments. Then at the 50th day, CPDs, phycoerythrin, chlorophyll a and MAAs (Palythine and asterina-330) were measured. The results showed that low doses UV-B radiation could significantly accelerate the growth rate of the tetraspores of C. ocellatus and the growth rate reduced gradually with UV-B radiation keep on increasing. The variation trend of both the phycoerythrin and chlorophyll a concentration reduced significantly (P < 0.05) once given the UVB radiation. Under UV-B stress, the CPDs were induced, and the concentrations of CPDs were significant low in dark repair groups. The data of MAAs analyzed by LC/MS in the tetraspores of C. ocellatus suggested that there were two MAAs formed (palythine and asterina-330) after UV-B radiation induced, and low dose of UV-B irradiation could significantly induced the concentration of MAAs. From the growth and development state of the tetraspores, negative effects of UV-B radiation on the dark repair groups were more serious than PAR.

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Qing Ju, Xuexi Tang & Xiaowei Zhao

  2. Tianjing Agricultural University, Tianjin, 300384, China

    Xiuquan Ren & Yunfei Li

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  1. Qing Ju
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Correspondence to Xuexi Tang.

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Ju, Q., Tang, X., Zhao, X. et al. Effects of UV-B radiation and different light repair conditions on the early development of the tetraspores of Chondrus ocellatus Holm. Acta Oceanol. Sin. 30, 100–111 (2011). https://doi.org/10.1007/s13131-011-0124-5

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