Abstract
Many anuran species remain to be formally named and described in Amazonia, notably in the Guiana Shield, and particularly in megadiverse groups such as Pristimantis. Several species in the Guiana Shield region have been confused with Pristimantis marmoratus and P. ockendeni. Hylodes grandoculis, a taxon previously placed in the synonymy of P. marmoratus, may be available for one of these species. To disentangle this confusing situation, we examined the external morphology and osteology (via µ-CT scans) of the holotype of H. grandoculis, the holotype of Pristimantis marmoratus, and of recently collected material for which we also analyzed molecular, acoustic, and morphological variation. We concluded that some populations from Suriname and northern Pará, Brazil, are distinct from P. marmoratus and correspond to Pristimantis grandoculis. Other populations, from French Guiana, are closely related to P. grandoculis but their status remains ambiguous. Finally, some populations, from French Guiana and Amapá, Brazil, are conspicuously distinct from both P. marmoratus and P. grandoculis and are described herein as P. crepitaculus sp. nov. A third species, belonging to a “trans-amazon complex”, occurs in southern Suriname, Guyana, and Brazil and remains undescribed. Pristimantis grandoculis and related populations from French Guiana lack external tympanum, columella, pharyngeal ostia, vocal slits and do not vocalize. This represents a rare, perhaps unique, example of a deaf and mute species of frogs from the Amazonian lowlands.
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All new DNA sequences, call recordings, and µ-CT scans have been deposited in public repositories.
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Acknowledgements
We thank Ate Alma-Cohen and Esther Dondorp from the Leiden Naturalis Biodiversity Center (National Research Institute for Biodiversity, The Netherlands), and Mark Wilkinson from the Natural History Museum (UK) who provided the µ-CT scans of the holotype of Hylodes grandoculis and the holotype of Pristimantis marmoratus, respectively. Renaud Lebrun and Quentin Martinez (ISEM) helped with data acquisition with the µ-CT facilities of the MRI platform member of the National Infrastructure France-BioImaging (supported by the French National Research Agency (ANR-10-INBS-04, «Investments for the future»), the Labex CEMEB (ANR-10-LABX-0004), and NUMEV (ANR-10-LABX-0020)). We also thank E. Courtois, L. Brandão, M. Dewynter, N. Vidal, J. Devillechabrole, J.-P. Vacher, P. Nunes, B. Villette, S. Cally, D. Baêta, P. Ouboter, V. Kadosoe, M. Blanc, J. Dias Lima, P. Gaucher, M.T. Rodrigues, and V. Rufray who helped to collect some of the specimens and tissue samples used in this work. Permission to conduct biodiversity research in Guyana was provided by the Environmental Protection Agency of Guyana under research permit number 180609 BR 112, and fieldwork was made possible through the Forestry Department of the Guyana Forestry Commission (GFC-PRDD), particularly R. Thomas. Collection permits for Brazil were provided by Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio).
Funding
This study benefited from an “Investissement d’Avenir” grant managed by the Agence Nationale de la Recherche (CEBA, rANR-10-LABX-25–01). PP and ATM were supported by fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (302501/2019–3 and 300822/2021–9 to PP; 142153/2019–2 to ATM). PP was also supported by an award from the Maxwell/Hanrahan Foundation. The work of RE was funded by the German Academic Exchange Service (DAAD) and Deutsche Forschungsgemeinschaft (DFG; ER 589/2–1). The work of PJRK was funded by the Fonds voor Wetenschappelijk Onderzoek (FWO12A7614N and FWO12A7617N).
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Appendices
Appendix 1 Molecular data
12S access | 16S access | Genus | Species | Voucher | Voucher2 | Locality | Country | lat | lon |
|---|---|---|---|---|---|---|---|---|---|
NA | JQ742162 | Pristimantis | abakapa | VUB3749 | Abakapa-tepui | Venezuela | 5.1892 | − 62.2944 | |
NA | JQ742163 | Pristimantis | abakapa | VUB3750 | Angasima-tepui | Venezuela | 5.0431 | − 62.0808 | |
NA | JQ742151 | Pristimantis | aureoventris | VUB3741 | Roraima-tepui | Guyana | 5.2500 | − 60.7167 | |
NA | JQ742152 | Pristimantis | aureoventris | VUB3748 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | JQ742153 | Pristimantis | aureoventris | VUB3742 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | JQ742154 | Pristimantis | aureoventris | VUB3747 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | JQ742155 | Pristimantis | aureoventris | VUB3744 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | JQ742156 | Pristimantis | aureoventris | VUB3745 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | JQ742157 | Pristimantis | aureoventris | VUB3746 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | JQ742158 | Pristimantis | aureoventris | VUB3743 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | JQ742159 | Pristimantis | aureoventris | VUB3499 | Wei-Assipu-tepui | Guyana | 5.2175 | − 60.7058 | |
NA | KDQF01000101 | Pristimantis | espedeus | AF0265 | Nouragues | French Guiana | 4.0917 | − 52.7000 | |
NA | KDQF01000107 | Pristimantis | espedeus | AF0277 | R116 | Nouragues | French Guiana | 4.0917 | − 52.7000 |
NA | KDQF01000108 | Pristimantis | espedeus | AF0278 | R117 | Nouragues | French Guiana | 4.0917 | − 52.7000 |
NA | KDQF01000150 | Pristimantis | espedeus | AF0576 | Itoupe | French Guiana | 3.0250 | − 53.0800 | |
NA | KDQF01000422 | Pristimantis | espedeus | AF1156 | R118 | Trinité | French Guiana | 4.6025 | − 53.4143 |
NA | KDQF01000423 | Pristimantis | espedeus | AF1157 | Trinité | French Guiana | 4.6025 | − 53.4143 | |
NA | KDQF01000439 | Pristimantis | espedeus | AF1190 | R119 | Trinité | French Guiana | 4.6025 | − 53.4143 |
NA | KDQF01000446 | Pristimantis | espedeus | AF1203 | R120 | Trinité | French Guiana | 4.6025 | − 53.4143 |
NA | KDQF01000452 | Pristimantis | espedeus | AF1219 | Trinité | French Guiana | 4.6025 | − 53.4143 | |
NA | KDQF01000570 | Pristimantis | espedeus | AF1520 | R121 | Grande Montagne Tortue | French Guiana | 4.2927 | − 52.3495 |
NA | KDQF01000571 | Pristimantis | espedeus | AF1521 | R122 | Grande Montagne Tortue | French Guiana | 4.2927 | − 52.3495 |
NA | KDQF01000572 | Pristimantis | espedeus | AF1522 | R123 | Grande Montagne Tortue | French Guiana | 4.29273 | − 52.3495 |
NA | KDQF01000952 | Pristimantis | espedeus | AF2430 | Nassau | Suriname | 4.8041 | − 54.5555 | |
NA | KDQF01000953 | Pristimantis | espedeus | AF2431 | Nassau | Suriname | 4.8041 | − 54.5555 | |
NA | KDQF01000955 | Pristimantis | espedeus | AF2433 | Nassau | Suriname | 4.8041 | − 54.5555 | |
NA | KDQF01001020 | Pristimantis | espedeus | AF2616 | Atachi Bakka | French Guiana | 3.5444 | − 53.9128 | |
NA | KDQF01001025 | Pristimantis | espedeus | AF2629 | Atachi Bakka | French Guiana | 3. 5444 | − 53.9128 | |
NA | KDQF01001028 | Pristimantis | espedeus | AF2632 | Atachi Bakka | French Guiana | 3. 5444 | − 53.9128 | |
NA | KDQF01001048 | Pristimantis | espedeus | AF2670 | Alikéné | French Guiana | 3.2156 | − 52.3980 | |
NA | KDQF01001057 | Pristimantis | espedeus | AF2684 | Alikéné | French Guiana | 3.2156 | − 52.3980 | |
NA | KDQF01001058 | Pristimantis | espedeus | AF2685 | Alikéné | French Guiana | 3.2156 | − 52.3980 | |
NA | KDQF01001061 | Pristimantis | espedeus | AF2690 | Alikéné | French Guiana | 3.2157 | − 52.3973 | |
NA | KDQF01001062 | Pristimantis | espedeus | AF2694 | Alikéné | French Guiana | 3.2157 | − 52.3973 | |
NA | KDQF01001063 | Pristimantis | espedeus | AF2695 | Alikéné | French Guiana | 3.2157 | − 52.3973 | |
NA | KDQF01001529 | Pristimantis | espedeus | AG268 | Lucifer | French Guiana | 4.7774 | − 53.9475 | |
NA | KDQF01001653 | Pristimantis | espedeus | AG507 | Saul | French Guiana | 3.7193 | − 53.4128 | |
NA | KDQF01001654 | Pristimantis | espedeus | AG508 | R124 | Saul | French Guiana | 3.7193 | − 53.4128 |
NA | KDQF01002024 | Pristimantis | espedeus | BPN2908 | RAP Basecamp 1 | Suriname | 2.5269 | − 55.7700 | |
JN690706 | JN691314 | Pristimantis | espedeus | CM395 | Lucifer | French Guiana | 4.7774 | − 53.9475 | |
NA | KDQF01003878 | Pristimantis | espedeus | PG477 | R131 | Kotika | French Guiana | 3.9333 | − 54.1972 |
NA | ON113943 | Pristimantis | espedeus | PG524 | R132 | Pic Coudreau de l'Est | French Guiana | 3.3005 | − 52.9493 |
NA | KDQF01004301 | Pristimantis | espedeus | ST300 | Parna Tumucumaque | AP | 0.9091 | − 53.2285 | |
NA | KDQF01004302 | Pristimantis | espedeus | ST301 | Parna Tumucumaque | AP | 0.9091 | − 53.2285 | |
NA | ON113944 | Pristimantis | espedeus | ST315 | Parna Tumucumaque | AP | 0.9091 | − 53.2285 | |
NA | ON113939 | Pristimantis | imthurni | PK3671 | Ptari-tepui | Venezuela | 5.7639 | − 61.8112 | |
NA | KDQF01000063 | Pristimantis | inguinalis | AF0157 | Brownsberg | Suriname | 4.9365 | − 55.1948 | |
NA | KDQF01000248 | Pristimantis | inguinalis | AF0803 | Kaw | French Guiana | 4.5507 | − 52.1610 | |
NA | KDQF01000253 | Pristimantis | inguinalis | AF0813 | Savane Virginie | French Guiana | 4.1959 | − 52.1490 | |
NA | KDQF01000256 | Pristimantis | inguinalis | AF0820 | Chutes Voltaire | French Guiana | 5.0312 | − 54.0878 | |
NA | KDQF01000295 | Pristimantis | inguinalis | AF0883 | Nouragues | French Guiana | 4.0850 | − 52.6810 | |
NA | KDQF01000514 | Pristimantis | inguinalis | AF1386 | Aratai | French Guiana | 3.9909 | − 52.5902 | |
NA | KDQF01000523 | Pristimantis | inguinalis | AF1415 | Savane Virginie | French Guiana | 4.1959 | − 52.1490 | |
NA | KDQF01000556 | Pristimantis | inguinalis | AF1498 | Nouragues | French Guiana | 4.0848 | − 52.6806 | |
NA | ON113918 | Pristimantis | inguinalis | AF1503 | Grande Montagne Tortue | French Guiana | 4.2927 | − 52.3496 | |
NA | KDQF01000609 | Pristimantis | inguinalis | AF1626 | Saul | French Guiana | 3.5732 | − 53.1985 | |
NA | KDQF01000814 | Pristimantis | inguinalis | AF2054 | Sipaliwini | Suriname | 2.1751 | − 56.0832 | |
NA | KDQF01000937 | Pristimantis | inguinalis | AF2398 | Nassau | Suriname | 4.8170 | − 54.6037 | |
NA | KDQF01000954 | Pristimantis | inguinalis | AF2432 | Nassau | Suriname | 4.8041 | − 54.5555 | |
NA | KDQF01001019 | Pristimantis | inguinalis | AF2613 | Atachi Bakka | French Guiana | 3.5520 | − 53.9509 | |
NA | KDQF01001043 | Pristimantis | inguinalis | AF2659 | Alikéné | French Guiana | 3.2091 | − 52.4020 | |
NA | KDQF01001121 | Pristimantis | inguinalis | AF2858 | Mitaraka | French Guiana | 2.2358 | − 54.4493 | |
NA | KDQF01001229 | Pristimantis | inguinalis | AF3083 | Mitan G | French Guiana | 2.6284 | − 52.5540 | |
NA | KDQF01001402 | Pristimantis | inguinalis | AF3404 | Bakhuis | Suriname | 4.6837 | − 56.7721 | |
NA | KDQF01001416 | Pristimantis | inguinalis | AF3432 | Bakhuis | Suriname | 4.6837 | − 56.7721 | |
NA | ON113921 | Pristimantis | inguinalis | AF3531 | Itoupe | French Guiana | 3.0230 | − 53.0955 | |
NA | ON113922 | Pristimantis | inguinalis | AF3772 | Voltzberg | Suriname | 4.6817 | − 56.1857 | |
NA | ON113923 | Pristimantis | inguinalis | AF4777 | Galbao | French Guiana | 4.4880 | − 52.0445 | |
NA | ON113925 | Pristimantis | inguinalis | AF5402 | Camp Savane Roche Dachine | French Guiana | 3.4696 | − 53.2300 | |
NA | ON113928 | Pristimantis | inguinalis | AF5448 | Trois-Saut | French Guiana | 2.1969 | − 52.9017 | |
NA | ON113929 | Pristimantis | inguinalis | AF5524 | Haute Courcibo | French Guiana | 4.4723 | − 53.2459 | |
NA | KDQF01001509 | Pristimantis | inguinalis | AG102 | Kaw | French Guiana | 4.5710 | − 52.2206 | |
NA | KDQF01001519 | Pristimantis | inguinalis | AG225 | Trinité | French Guiana | 4.6709 | − 53.2843 | |
JN690710 | JN691317 | Pristimantis | inguinalis | AG229 | Trinité | French Guiana | 4.6709 | − 53.2843 | |
NA | KDQF01001538 | Pristimantis | inguinalis | AG291 | Trinité | French Guiana | 4.6025 | − 53.4143 | |
NA | KDQF01001595 | Pristimantis | inguinalis | AG424 | Atachi Bakka | French Guiana | 3.6553 | − 53.8440 | |
NA | KDQF01001643 | Pristimantis | inguinalis | AG491 | Saul | French Guiana | 3.7193 | − 53.4128 | |
NA | KDQF01001691 | Pristimantis | inguinalis | AM015 | Inini Tolenga | French Guiana | 3.6632 | − 53.9283 | |
NA | KDQF01002875 | Pristimantis | inguinalis | MPEG30059 | Serra do Acari. N ESEC Grão Pará | PA | 1.2854 | − 58.6959 | |
NA | KDQF01002876 | Pristimantis | inguinalis | MPEG30060 | Serra do Acari. N ESEC Grão Pará | PA | 1.2854 | − 58.6959 | |
NA | KDQF01003609 | Pristimantis | inguinalis | MTR24299 | Oiapoque | AP | 3.8794 | − 51.7710 | |
NA | KDQF01003778 | Pristimantis | inguinalis | NZCS51 | Brownsberg | Suriname | 4.9365 | − 55.194826 | |
NA | KDQF01004105 | Pristimantis | inguinalis | QM0406 | Montagne de fer | French Guiana | 5.4074 | − 53.5548 | |
NA | KDQF01004107 | Pristimantis | inguinalis | QM0426 | Montagne de fer | French Guiana | 5.4074 | − 53.5548 | |
NA | ON113942 | Pristimantis | inguinalis | QM1074 | Gaa_Kaba | French Guiana | 4.4509 | − 54.4117 | |
NA | JQ742170 | Pristimantis | inguinalis | VUB3626 | Cacao Mountain | French Guiana | 4.5667 | − 52.4667 | |
EU186739 | EU186721 | Pristimantis | jamescameroni | SBH268110 | Bolivar, Aprada-tepui | Venezuela | 5.4243 | − 62.4208 | |
NA | ON113932 | Pristimantis | jamescameroni | IRSNB4163 | Bolivar, Aprada-tepui | Venezuela | 5.4243 | − 62.4208 | |
NA | JQ742169 | Pristimantis | jester | VUB3493 | Maringma-tepui | Guyana | 5.21524 | − 60.5834 | |
NA | MF037223 | Pristimantis | marmoratus | CPI10703 | Mt. Wokomung | Guyana | 5.1175 | − 59.8225 | |
EU186734 | EU186716 | Pristimantis | marmoratus | ROM43302 | Mt. Wokomung | Guyana | 5.1175 | − 59.8225 | |
EU186741 | EU186723 | Pristimantis | marmoratus | KU181015 | km 127, El Dorado-Santa Elena de Uairen Rd | Venezuela | 5.8983 | − 61.4428 | |
NA | JQ742166 | Pristimantis | marmoratus | VUB3485 | Kaieteur NP | Guyana | 5.2686 | − 59.7686 | |
NA | JQ742167 | Pristimantis | marmoratus | VUB3491 | Maringma-tepui | Guyana | 5.2152 | − 60.5834 | |
NA | KDQF01002108 | Pristimantis | marmoratus | BPN3814 | Bay Camp | Guyana | 5.0112 | − 59.63930 | |
NA | KDQF01002109 | Pristimantis | marmoratus | BPN3815 | Bay Camp | Guyana | 5.0112 | − 59.6393 | |
NA | KDQF01002110 | Pristimantis | marmoratus | BPN3816 | Bay Camp | Guyana | 5.0112 | − 59.6393 | |
NA | JQ742164 | Pristimantis | pulvinatus | VUB3751 | Iwokrama | Guyana | 4.6714 | − 58.6850 | |
NA | JQ742165 | Pristimantis | pulvinatus | VUB3674 | La Escalera | Venezuela | 5.9158 | − 61.4347 | |
NA | KDQF01004007 | Pristimantis | pulvinatus | PK3509 | Iwokrama | Guyana | 4.6720 | − 58.6840 | |
EU186692 | EU186692 | Pristimantis | saltissimus | ROM43913 | Mt. Ayanganna | Guyana | 5.3996 | − 59.9505 | |
EU186693 | EU186693 | Pristimantis | saltissimus | ROM43310 | Mt. Wokomung | Guyana | 5.1175 | − 59.8225 | |
NA | JQ742168 | Pristimantis | saltissimus | VUB3490 | Maringma-tepui | Guyana | 5.2152 | − 60.5834 | |
JN690708 | JN691315 | Pristimantis | crepitaculus | AF0021 | Saul | French Guiana | 3.6256 | − 53.2072 | |
NA | KR811102 | Pristimantis | crepitaculus | AF0563 | Savane Virginie | French Guiana | 4.1959 | − 52.1490 | |
NA | KR811105 | Pristimantis | crepitaculus | AF0757 | St Georges | French Guiana | 3.8112 | − 51.8896 | |
NA | KDQF01000316 | Pristimantis | crepitaculus | AF0910 | MNHN-RA-2020.0093 | Pic Coudreau du Sud | French Guiana | 2.2534 | − 54.3534 |
NA | KDQF01000366 | Pristimantis | crepitaculus | AF1014 | Saul | French Guiana | 3.6359 | − 53.2726 | |
NA | KDQF01000382 | Pristimantis | crepitaculus | AF1046 | Saul | French Guiana | 3.6112 | − 53.2886 | |
NA | ON113933 | Pristimantis | crepitaculus | AF1387 | MNHN-RA-2020.0094 | Aratai | French Guiana | 3.9909 | − 52.5902 |
NA | ON113934 | Pristimantis | crepitaculus | AF1388 | MNHN-RA-2020.0095 | Aratai | French Guiana | 3.9909 | − 52.5902 |
NA | ON113935 | Pristimantis | crepitaculus | AF1598 | MNHN-RA-2020.0097 | Nouragues | French Guiana | 4.0848 | − 52.6806 |
NA | KDQF01000601 | Pristimantis | crepitaculus | AF1607 | MNHN-RA-2020.0100 | Saul | French Guiana | 3.5732 | − 53.1985 |
NA | KDQF01000749 | Pristimantis | crepitaculus | AF1921 | MNHN-RA-2020.0101 | Flat de la Waki | French Guiana | 3.0840 | − 53.4107 |
NA | KDQF01000754 | Pristimantis | crepitaculus | AF1941 | Flat de la Waki | French Guiana | 3.0836 | − 53.4121 | |
NA | KDQF01001103 | Pristimantis | crepitaculus | AF2786 | MNHN-RA-2020.0102 | Mitaraka | French Guiana | 2.2358 | − 54.4493 |
NA | ON113936 | Pristimantis | crepitaculus | AF2799 | MNHN-RA-2020.0103 | Mitaraka | French Guiana | 2.2358 | − 54.4493 |
NA | KDQF01001186 | Pristimantis | crepitaculus | AF3002 | Mémora D | AP | 3.3129 | − 52.1804 | |
NA | KDQF01001205 | Pristimantis | crepitaculus | AF3047 | Mitan G | French Guiana | 2.6284 | − 52.5540 | |
NA | KDQF01001276 | Pristimantis | crepitaculus | AF3161 | MNHN-RA-2020.0108 | Mitan D | AP | 2.6276 | − 52.5419 |
NA | KDQF01001437 | Pristimantis | crepitaculus | AF3539 | MNHN-RA-2020.0092 | Itoupe | French Guiana | 3.0230 | − 53.0955 |
NA | ON113924 | Pristimantis | crepitaculus | AF5399 | Camp Saut Richard | French Guiana | 3.47363 | − 53.2086 | |
NA | ON113927 | Pristimantis | crepitaculus | AF5440 | Trois-Saut | French Guiana | 2.1969 | − 52.9017 | |
JN690707 | EU201063 | Pristimantis | crepitaculus | AG_BM110 | Kaw3 | French Guiana | 4.5481 | − 52.1519 | |
NA | KDQF01001542 | Pristimantis | crepitaculus | AG321 | Kaw | French Guiana | 4.5161 | − 52.1005 | |
NA | KDQF01001568 | Pristimantis | crepitaculus | AG378 | Montagne Maripa | French Guiana | 4.4088 | − 52.34301 | |
NA | KDQF01001645 | Pristimantis | crepitaculus | AG494 | Saul | French Guiana | 3.7193 | − 53.4128 | |
NA | KDQF01001886 | Pristimantis | crepitaculus | BOAM048 | Borne 4 | French Guiana | 2.3709 | − 53.7728 | |
NA | KDQF01002129 | Pristimantis | crepitaculus | CAAM15 | Montagne Cacao | French Guiana | 2.3491 | − 53.2160 | |
NA | EU201062 | Pristimantis | crepitaculus | CM077 | Trois-Sauts | French Guiana | 2.2480 | − 52.8746 | |
NA | KDQF01002243 | Pristimantis | crepitaculus | CM171 | Trijonction | French Guiana | 2.3333 | − 54.6000 | |
NA | KDQF01002504 | Pristimantis | crepitaculus | FL04 | Plote PPBIO | AP | 0.9793 | − 51.6149 | |
NA | KDQF01002521 | Pristimantis | crepitaculus | FTA128 | Rio Vila Nova | AP | 0.4493 | − 52.0221 | |
NA | KDQF01002528 | Pristimantis | crepitaculus | FTA165 | Rio Amapá Grande | AP | 2.1271 | − 51.1913 | |
NA | KDQF01002543 | Pristimantis | crepitaculus | FTA239 | Rio Amapá Grande | AP | 2.1271 | − 51.1913 | |
NA | ON113931 | Pristimantis | crepitaculus | FTA96 | Rio Vila Nova | AP | 0.4493 | − 52.0221 | |
NA | KDQF01003282 | Pristimantis | crepitaculus | MTR13884 | Lourenço | AP | 2.32361 | − 51.6453 | |
NA | KDQF01003293 | Pristimantis | crepitaculus | MTR13930 | Lourenço | AP | 2.32361 | − 51.6453 | |
NA | KR811104 | Pristimantis | crepitaculus | MTR24285 | Lourenço | AP | 2.3216 | − 51.6109 | |
NA | KR811103 | Pristimantis | crepitaculus | MTR24300 | Oiapoque | AP | 3.8794 | − 51.7710 | |
NA | KDQF01003952 | Pristimantis | crepitaculus | PG748 | St Georges | French Guiana | 3.8061 | − 51.8934 | |
NA | KDQF01004462 | Pristimantis | crepitaculus | WAAM011 | Flat de la Waki | French Guiana | 3.18341 | − 53.4775 | |
NA | KDQF01003322 | Pristimantis | sp. Neblina 1 | MTR15532 | São Gabriel da Cachoeira | AM | 0.7195 | − 66.0878 | |
NA | KDQF01003323 | Pristimantis | sp. Neblina 1 | MTR15534 | São Gabriel da Cachoeira | AM | 0.7195 | − 66.0878 | |
NA | KDQF01003325 | Pristimantis | sp. Neblina 2 | MTR15536 | São Gabriel da Cachoeira | AM | 0.7195 | − 66.0878 | |
NA | KDQF01000102 | Pristimantis | sp. “Guianas” East | AF0266 | Nouragues | French Guiana | 4.0917 | − 52.7000 | |
NA | KDQF01000103 | Pristimantis | sp. “Guianas” East | AF0268 | Nouragues | French Guiana | 4.0917 | − 52.7000 | |
NA | KDQF01000104 | Pristimantis | sp. “Guianas” East | AF0272 | Nouragues | French Guiana | 4.0917 | − 52.700 | |
NA | KDQF01000354 | Pristimantis | sp. “Guianas” East | AF0978 | Saul | French Guiana | 3.6359 | − 53.2726 | |
NA | ON113917 | Pristimantis | sp. “Guianas” East | AF1360 | Grande Montagne Tortue | French Guiana | 4.2927 | − 52.3496 | |
NA | KDQF01000558 | Pristimantis | sp. “Guianas” East | AF1505 | Grande Montagne Tortue | French Guiana | 4.2927 | − 52.3496 | |
NA | KDQF01001024 | Pristimantis | sp. “Guianas” East | AF2625 | Atachi Bakka | French Guiana | 3.5444 | − 53.9128 | |
NA | ON113919 | Pristimantis | sp. “Guianas” East | AF2626 | Atachi Bakka | French Guiana | 3.5444 | − 53.9128 | |
NA | ON113920 | Pristimantis | sp. “Guianas” East | AF2818 | Mitaraka | French Guiana | 2.2358 | − 54.4493 | |
NA | ON113926 | Pristimantis | sp. “Guianas” East | AF5414 | Savane Roche Dachine | French Guiana | 3.4696 | − 53.2298 | |
NA | ON113930 | Pristimantis | sp. “Guianas” East | AF5525 | Haute Courcibo | French Guiana | 4.4723 | − 53.2459 | |
NA | KDQF01001850 | Pristimantis | sp. “Guianas” East | BOAM001 | Borne 4 | French Guiana | 2.3709 | − 53.7728 | |
NA | KDQF01001860 | Pristimantis | sp. “Guianas” East | BOAM012 | Borne 4 | French Guiana | 2.3709 | − 53.7728 | |
NA | KDQF01002249 | Pristimantis | sp. “Guianas” East | CM184 | Trijonction | French Guiana | 2.3333 | − 54.6000 | |
JN690709 | JN691316 | Pristimantis | sp. “Guianas” East | CM317 | CisameG | French Guiana | 4.1833 | − 52.3667 | |
NA | ON113940 | Pristimantis | sp. “Guianas” East | QM0423 | Montagne de fer | French Guiana | 5.4074 | − 53.5548 | |
NA | ON113941 | Pristimantis | sp. “Guianas” East | QM1073 | Gaa Kaba | French Guiana | 4.4520 | − 54.4193 | |
ON117803 | KDQF01004305 | Pristimantis | sp. “Guianas” East | ST310 | Parna Tumucumaque | AP | 0.9091 | − 53.2285 | |
NA | KDQF01004306 | Pristimantis | sp. “Guianas” East | ST311 | Parna Tumucumaque | AP | 0.9091 | − 53.2285 | |
NA | ON113937 | Pristimantis | grandoculis | UFRJA024 | NZCS A1211 | Lelygebergte | Suriname | 4.4158 | − 54.6498 |
NA | ON113938 | Pristimantis | grandoculis | UFRJA023 | NZCS A1210 | Lelygebergte | Suriname | 4.4158 | − 54.6498 |
NA | KDQF01002880 | Pristimantis | grandoculis | MPEG30085 | Serra do Acari | PA | 1.2854 | − 58.6959 | |
NA | KDQF01002881 | Pristimantis | grandoculis | MPEG30088 | Serra do Acari | PA | 1.2854 | − 58.6959 | |
NA | KDQF01003777 | Pristimantis | grandoculis | NZCS50 | Brownsberg | Suriname | 4.9366 | − 55.1948 | |
NA | KDQF01003795 | Pristimantis | grandoculis | NZCS A1019 | Tafelberg | Suriname | 3.8049 | − 56.1539 | |
NA | KDQF01003796 | Pristimantis | grandoculis | NZCS A1212 | Tafelberg | Suriname | 3.8049 | − 56.1539 | |
NA | KDQF01001388 | Pristimantis | grandoculis | AF3365 | Bakhuis | Suriname | 4.6562 | − 56.7865 | |
NA | KDQF01001400 | Pristimantis | grandoculis | AF3402 | Bakhuis | Suriname | 4.6837 | − 56.7721 | |
NA | KDQF01001403 | Pristimantis | grandoculis | AF3408 | Bakhuis | Suriname | 4.6837 | − 56.7721 | |
NA | KDQF01001417 | Pristimantis | grandoculis | AF3435 | MNHN-RA-2020.0118 | Bakhuis | Suriname | 4.6837 | − 56.7721 |
EU186676 | EU186676 | Pristimantis | sp. Ayangana | ROM40164 | Mt. Ayanganna | Guyana | 5.3995 | − 59.9505 | |
ON117804 | KDQF01004221 | Pristimantis | cf. ockendeni | SMNS11989 | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | |
ON117805 | KDQF01004222 | Pristimantis | cf. ockendeni | SMNS11990 | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | |
ON117806 | KDQF01004223 | Pristimantis | cf. ockendeni | SMNS11994 | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | |
ON117807 | KDQF01000865 | Pristimantis | cf. ockendeni | AF2155 | Sipaliwini | Suriname | 2.02682 | − 56.1256 | |
NA | KDQF01003210 | Pristimantis | cf. ockendeni | MTR12690 | Igarapé Açu, Rio Abacaxis | AM | − 4.3442 | − 58.6350 | |
NA | KDQF01001829 | Pristimantis | cf. ockendeni | BM153 | UHE Belo Monte, Vitória do Xingu, Rio Xingu | PA | − 2.9506 | − 51.9366 | |
NA | KDQF01004256 | Pristimantis | cf. ockendeni | SMS155 | Campo Catuquira | AM | − 4.9136 | − 61.1092 | |
NA | KDQF01004257 | Pristimantis | cf. ockendeni | SMS156 | Campo Catuquira | AM | − 4.9136 | − 61.1092 | |
NA | KDQF01002705 | Pristimantis | cf. ockendeni | JOG847 | MINA PALITO | PA | − 6.3173 | − 55.7986 | |
NA | KDQF01003224 | Pristimantis | cf. ockendeni | MTR12855 | São Sebastião, Rio Abacaxis | AM | − 4.3089 | − 58.6364 | |
NA | KDQF01000889 | Pristimantis | cf. ockendeni | AF2257 | Sipaliwini | Suriname | 2.0244 | − 56.1251 | |
NA | KDQF01002605 | Pristimantis | cf. ockendeni | H2773 | UHE Jirau | RO | − 9.4459 | − 64.8247 | |
NA | JQ742160 | Pristimantis | yuruaniensis | VUB3717 | Yuruani-tepui | Venezuela | 5.3139 | − 60.8639 | |
NA | JQ742161 | Pristimantis | yuruaniensis | VUB3720 | Yuruani-tepui | Venezuela | 5.3139 | − 60.8639 |
Appendix 2 Specimens examined and types
Genus | Species | Museum | Field N | Sex | Type | Locality | Country | Lat | Lon | Collector | Date | Acoustic |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Pristimantis | crepitaculus | MNHN-RA-2020.0093 | AF0910 | F | Paratype | Pic Coudreau du Sud | French Guiana | 2.2534 | − 54.3534 | M. Blanc | 01_02_2013 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0104 | AF2815 | F | Paratype | Mitaraka | French Guiana | 2.2358 | − 54.4493 | A. Fouquet − M. Dewynter − N. Vidal | 28_02_2015 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0105 | AF2816 | F | Paratype | Mitaraka | French Guiana | 2.2358 | − 54.4493 | A. Fouquet − M. Dewynter − N. Vidal | 28_02_2015 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0094 | AF1387 | M | Paratype | Aratai | French Guiana | 3.9909 | − 52.5902 | A. Fouquet − J. Devillechabrolle | 16_12_2013 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0095 | AF1388 | M | Paratype | Aratai | French Guiana | 3.9909 | − 52.5902 | A. Fouquet − J. Devillechabrolle | 16_12_2013 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0096 | AF1597 | M | Paratype | Nouragues | French Guiana | 4.0848 | − 52.6806 | E. Courtois | 09_02_2014 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0097 | AF1598 | M | Paratype | Nouragues | French Guiana | 4.08489 | − 52.6806 | E. Courtois | 09_02_2014 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0098 | AF1599 | M | Paratype | Nouragues | French Guiana | 4.08489 | − 52.6806 | E. Courtois | 09_02_2014 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0099 | AF1606 | M | Paratype | Saul | French Guiana | 3.5732 | − 53.1985 | A. Fouquet − E. Courtois − J.P. Vacher | 20_02_2014 | MNHN-SO-2022-559 |
Pristimantis | crepitaculus | MNHN-RA-2020.0100 | AF1607 | M | Paratype | Saul | French Guiana | 3.5732 | − 53.1985 | A. Fouquet − E. Courtois − J.P. Vacher | 20_02_2014 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0101 | AF1921 | M | Paratype | Flat de la Waki | French Guiana | 3.0840 | − 53.4107 | J.P. Vacher | 07_04_2014 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0102 | AF2786 | M | Holotype | Mitaraka | French Guiana | 2.2358 | − 54.4493 | A. Fouquet − M. Dewynter − N. Vidal | 26_02_2015 | MNHN-SO-2019–23 |
Pristimantis | crepitaculus | MNHN-RA-2020.0103 | AF2799 | M | Paratype | Mitaraka | French Guiana | 2.2358 | − 54.4493 | A. Fouquet − M. Dewynter − N. Vidal | 27_02_2015 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0106 | AF2860 | M | Paratype | Mitaraka | French Guiana | 2.2358 | − 54.4493 | A. Fouquet − M. Dewynter − N. Vidal | 04_03_2015 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0107 | AF3054 | M | Paratype | Mitan G | French Guiana | 2.6284 | − 52.5540 | A. Fouquet − P. Nunes | 20_03_2015 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0108 | AF3161 | M | Paratype | Mitan D | AP | 2.6276 | − 52.5419 | A. Fouquet − P. Nunes | 23_03_2015 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0109 | AF3530 | M | Paratype | Itoupe | French Guiana | 3.0230 | − 53.0955 | A Fouquet − E Courtois − B Villette − M Dewynter | 06_01_2016 | MNHN-SO-2022-561 |
Pristimantis | crepitaculus | MNHN-RA-2020.0092 | AF3539 | M | Paratype | Itoupe | French Guiana | 3.0230 | − 53.0955 | A Fouquet − E Courtois − B Villette − M Dewynter | 06_01_2016 | MNHN-SO-2022-560 |
Pristimantis | crepitaculus | MNHN-RA-2020.0110 | AF3548 | M | Paratype | Itoupe | French Guiana | 3.02218 | − 53.1105 | A Fouquet − E Courtois − B Villette − M Dewynter | 06_01_2016 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0111 | AF3664 | M | Paratype | Itoupe | French Guiana | 3.0256 | − 53.0525 | A Fouquet − E Courtois − B Villette − M Dewynter | 11_01_2016 | |
Pristimantis | crepitaculus | MNHN-RA-2020.0112 | AF3687 | M | Paratype | Itoupe | French Guiana | 3.0230 | − 53.0955 | A Fouquet − E Courtois − B Villette − M Dewynter | 14_01_2016 | |
Pristimantis | grandoculis | MNHN-RA-2020.0118 | AF3435 | M | Bakhuis | Suriname | 4.7246 | − 56.7638 | A. Fouquet − S. Cally − R. Jairam | 30_04_2015 | ||
Pristimantis | grandoculis | RMNH 4467 | M | Holotype | Coppenname | Suriname | ||||||
Pristimantis | grandoculis | NZCSA1212 | M | Tafleberg | Suriname | 3.8049 | − 56.1539 | P. Ouboter − V. Kadosoe | ||||
Pristimantis | crepitaculus | MPEG 41,819 | PLVP 289 | M | Paratype | Oiapoque | Brazil | 3.79889 | − 51.87993 | P. Peloso − L. Brandão | 04_12_2015 | |
Pristimantis | crepitaculus | MPEG 41,820 | PLVP 290 | M | Paratype | Oiapoque | Brazil | 3.79889 | − 51.87993 | P. Peloso − L. Brandão | 05_12_2015 | |
Pristimantis | crepitaculus | MPEG 41,821 | PLVP 301 | M | Paratype | Oiapoque | Brazil | 3.79889 | − 51.87993 | P. Peloso − L. Brandão | 05_12_2015 | |
Pristimantis | crepitaculus | MPEG 41,822 | PLVP 352 | M | Paratype | Oiapoque | Brazil | 3.79889 | − 51.87993 | P. Peloso − L. Brandão | 06_12_2015 | MNHN-SO-2022-562 |
Pristimantis | crepitaculus | MPEG 41,823 | PLVP 353 | M | Paratype | Oiapoque | Brazil | 3.79889 | − 51.87993 | P. Peloso − L. Brandão | 06_12_2015 | MNHN-SO-2022-563 |
Pristimantis | crepitaculus | MPEG 41,824 | PLVP 354 | M | Paratype | Oiapoque | Brazil | 3.79889 | − 51.87993 | P. Peloso − L. Brandão | 06_12_2015 | |
Pristimantis | crepitaculus | MPEG 41,825 | PLVP 355 | F | Paratype | Oiapoque | Brazil | 3.79889 | − 51.87993 | P. Peloso − L. Brandão | 06_12_2015 | |
Pristimantis | grandoculis | NZCS A1210 | UFRJA023 | M | Lely Mountain | Suriname | 4.2667 | − 54.7333 | R. Jairam – D. Baeta | |||
Pristimantis | grandoculis | NZCS A1211 | UFRJA024 | M | Lely Mountain | Suriname | 4.2667 | − 54.7333 | R. Jairam – D. Baeta | |||
Pristimantis | cf. ockendeni | MNHN-RA-2020.0113 | AF2155 | F | Sipaliwini | Suriname | 2.0268 | − 56.1256 | A. Fouquet − J.P. Vacher | 23_04_2014 | ||
Pristimantis | cf. ockendeni | MNHN-RA-2020.0114 | AF2258 | F | Sipaliwini | Suriname | 2.0244 | − 56.1251 | A. Fouquet − J.P. Vacher | 27_04_2014 | ||
Pristimantis | cf. ockendeni | MTD47769 | F | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 18 08 2010 | |||
Pristimantis | cf. ockendeni | SMNS11990 | F | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 25 04 2003 | |||
Pristimantis | cf. ockendeni | SMNS11991 | F | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 25 04 2003 | |||
Pristimantis | cf. ockendeni | SMNS11995 | F | Mabura Hill forest reserve | Guyana | 5.15538 | − 58.6997 | R Ernst | 01 06 2004 | |||
Pristimantis | cf. ockendeni | MNHN-RA-2020.0115 | AF2255 | M | Sipaliwini | Suriname | 2.0244 | − 56.12518 | A. Fouquet − J.P. Vacher | 27_04_2014 | MNHN-SO-2021-XXX | |
Pristimantis | cf. ockendeni | MNHN-RA-2020.0116 | AF2256 | M | Sipaliwini | Suriname | 2.02449 | − 56.1251 | A. Fouquet − J.P. Vacher | 27_04_2014 | ||
Pristimantis | cf. ockendeni | MNHN-RA-2020.0117 | AF2289 | M | Sipaliwini | Suriname | 2.0334 | − 56.1318 | A. Fouquet − J.P. Vacher | 30_04_2014 | MNHN-SO-2021-XXX | |
Pristimantis | cf. ockendeni | MTD47770 | M | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 18 08 2010 | |||
Pristimantis | cf. ockendeni | SMNS11987 | M | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 18 11 2002 | |||
Pristimantis | cf. ockendeni | SMNS11989 | M | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 17 04 2003 | |||
Pristimantis | cf. ockendeni | SMNS11993 | M | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 26 03 2004 | |||
Pristimantis | cf. ockendeni | SMNS11994 | M | Mabura Hill forest reserve | Guyana | 5.1553 | − 58.6997 | R Ernst | 01 06 2004 |
Appendix 3 Call recordings list
Pristimantis crepitaculus
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PLPDR 126 (MPEG 41822), Paratype, Oiapoque – Amapá, Brazil MNHN-SO-2022-562
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PLPDR 128 (MPEG 41823), Paratype, Oiapoque – Amapá, Brazil MNHN-SO-2022-563
-
track59 Marty and Gaucher (NA), Kaw montain − French Guiana
-
160106_0692 (AF3530), Itoupé − French Guiana MNHN-SO-2022-561
-
160106_0693(AF3539), Holotype, Itoupé − French Guiana MNHN-SO-2022-560
-
LS110010 (AF1606), Saul Limonade − French Guiana MNHN-SO-2022-559
Appendix 4
Mean pairwise p distance
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Fouquet, A., Peloso, P., Jairam, R. et al. Back from the deaf: integrative taxonomy revalidates an earless and mute species, Hylodes grandoculis van Lidth de Jeude, 1904, and confirms a new species of Pristimantis Jiménez de la Espada, 1870 (Anura: Strabomantidae) from the Eastern Guiana Shield. Org Divers Evol 22, 1065–1098 (2022). https://doi.org/10.1007/s13127-022-00564-w
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DOI: https://doi.org/10.1007/s13127-022-00564-w