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Caveolin-1 knockout mice have altered serum N-glycan profile and sialyltransferase tissue expression

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Abstract

Caveolin-1 (Cav-1) is a constitutive protein within caveolar membranes. Previous studies from our group and others indicated that Cav-1 could mediate N-glycosylation, α2,6-sialylation, and fucosylation in mouse hepatocarcinoma cells in vitro. However, little is known about the effect of Cav-1 expression on glycosylation modifications in vivo. In this study, the N-glycan profiles in serum from Cav-1−/− mice were investigated by lectin microarray and mass spectrometric analysis approaches. The results showed that levels of multi-antennary branched, α2,6-sialylated, and galactosylated N-glycans increased, while high-mannose typed and fucosylated N-glycans decreased in the serum of Cav-1−/− mice, compared with that of wild-type mice. Furthermore, the real-time quantitative PCR analysis indicated that α2,6-sialyltransferase gene expression decreased significantly in Cav-1−/− mouse organ tissues, but α2,3- and α2,8-sialyltransferase did not. Of them, both mRNA and protein expression levels of the β-galactoside α2,6-sialyltransferase 1 (ST6Gal-I) had dramatically reduced in Cav-1−/− mice organ tissues, which was consistent with the α2,6-sialyl Gal/GalNAc level reduced significantly in tissues instead of serum from Cav-1−/− mice. These results provide for the first time the N-glycans profile of Cav-1−/− mice serum, which will facilitate understanding the function of Cav-1 from the perspective of glycosylation.

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Funding

This research was supported by the Major State Basic Research Development Program of China (No. 2012CB822103), the National Natural Science Foundation of China (No. 31570802).

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Authors and Affiliations

  1. School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin, 124221, Liaoning, China

    Xixi Chen, Liping Wang & Jianing Zhang

  2. Department of Biochemistry and Molecular Biology, Institute of Glycobiology, Dalian Medical University, Dalian, 116044, Liaoning, China

    Yinshuang Wu, Hongshuo Zhang, Weijie Dong & Shujing Wang

  3. Department of Pathology, Dalian Medical University, Dalian, 116044, Liaoning, China

    Xiao Yu

  4. Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Chuncui Huang & Yan Li

  5. University of The Chinese Academy of Sciences, Beijing, 100049, China

    Yan Li

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  1. Xixi Chen
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Contributions

JNZ designed the research study; XXC, LPW, YSW, HSZ, WJD, XY, CCH, and YL performed the experiments; XXC, LPW, CCH, YL analyzed the data; XXC, SJW, and JNZ wrote the manuscript. XXC, LPW, SJW, JNZ revised the manuscript. The authors declare that all data were generated in-house and that no paper mill was used.

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Correspondence to Jianing Zhang.

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Chen, X., Wang, L., Wu, Y. et al. Caveolin-1 knockout mice have altered serum N-glycan profile and sialyltransferase tissue expression. J Physiol Biochem 78, 73–83 (2022). https://doi.org/10.1007/s13105-021-00840-x

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