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GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells

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Abstract

The mammary gland increases energy requirements during pregnancy and lactation to support epithelial proliferation and milk nutrients synthesis. Lactose, the principal carbohydrate of the milk, is synthetized in the Golgi of mammary epithelial cells by lactose synthase from glucose and UPD galactose. We studied the temporal changes in the expression of GLUT1 and GLUT8 in mammary gland and their association with lactose synthesis and proliferation in BALB/c mice. Six groups were used: virgin, pregnant at 2 and 17 days, lactating at 2 and 10 days, and weaning at 2 days. Temporal expression of GLUT1 and GLUT8 transporters by qPCR, western blot and immunohistochemistry, and its association with lactalbumin, Ki67, and cytokeratin 18 within mammary tissue was studied, along with subcellular localization. GLUT1 and GLUT8 transporters increased their expression during mammary gland progression, reaching 20-fold increasing in GLUT1 mRNA at lactation (p < 0.05) and 2-fold at protein level for GLUT1 and GLUT8 (p < 0.05 and 0.01, respectively). The temporal expression pattern was shared with cytokeratin 18 and Ki67 (p < 0.01). Endogenous GLUT8 partially co-localized with 58 K protein and α-lactalbumin in mammary tissue and with Golgi membrane–associated protein 130 in isolated epithelial cells. The spatial-temporal synchrony between expression of GLUT8/GLUT1 and alveolar cell proliferation, and its localization in cis-Golgi associated to lactose synthase complex, suggest that both transporters are involved in glucose uptake into this organelle, supporting lactose synthesis.

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Acknowledgments

The authors thank Mrs. Katia Muñoz, Fresia Jarpa, and Mr. Rafael Maura for technical support, and Mr. George Montgomery for manuscript editing. In memory of Dr. Juan Carlos Vera, who deceased during the development of this research.

Funding

This work was supported by Fondo de Desarrollo Científico y Tecnológico (FONDECYT), Chilean Government [grants 11121367 to LM and 3150285 to MV].

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Authors and Affiliations

  1. Departamento de Ciencias Básicas, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Alonso de Ribera 2850, 4090541, Concepción, Chile

    Marcelo Villagrán, Mirna Muñoz, Camila Venegas, Iván Ruminot, Esteban Parra-Valencia, Reginald del Pozo & Lorena Mardones

  2. Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Laboratorio de Antioxidantes, Universidad de Concepción. Barrio Universitario s/n, Box 160-C, Concepción, Chile

    Marcelo Villagrán, Eveling Inostroza, Mafalda Maldonado, Coralia I. Rivas & Juan Carlos Vera

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  1. Marcelo Villagrán
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  2. Mirna Muñoz
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  3. Eveling Inostroza
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  7. Mafalda Maldonado
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  10. Juan Carlos Vera
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  11. Lorena Mardones
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Corresponding author

Correspondence to Lorena Mardones.

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The Institutional Ethic Committee approved all procedures performed in animals conformed to the Guide of the Care and Use of Laboratory Animals of the National Council for Science and Technology Research (CONICYT, Chile).

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Villagrán, M., Muñoz, M., Inostroza, E. et al. GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells. J Physiol Biochem 75, 209–215 (2019). https://doi.org/10.1007/s13105-019-00679-3

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  • DOI: https://doi.org/10.1007/s13105-019-00679-3

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