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Effects of exendin-4 and selenium on the expression of GLP-1R, IRS-1, and preproinsulin in the pancreas of diabetic rats

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An Erratum to this article was published on 05 October 2017

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Abstract

The mechanisms by which exendin-4 and selenium exert their antidiabetic actions are still unclear. Here, we investigated the effects of exendin-4 or selenium administration on the expression of glucagon-like peptide-1 receptor (GLP-1R), insulin receptor substrate-1 (IRS-1), and preproinsulin in the pancreas of diabetic rats. Diabetes was induced by streptozotocin administration. Diabetic rats were injected intraperitoneally with 0.03 μg exendin-4/kg body weight/daily or treated with 5 ppm selenium in drinking water for a period of 4 weeks. GLP-1R and IRS-1 levels were decreased while the level of preproinsulin messenger RNA (mRNA) was increased in the pancreas of diabetic untreated rats, as compared to that in control rats. Treatment of diabetic rats with exendin-4 increased protein and mRNA levels of GLP-1R, and IRS-1, and the mRNA level of preproinsulin in the pancreas, as compared to their levels in diabetic untreated rats. Selenium treatment of diabetic rats increased the pancreatic mRNA levels of GLP-1R, IRS-1, and preproinsulin. Exendin-4 or selenium treatment of diabetic rats also increased the numbers of pancreatic islets and GLP-1R molecules in the pancreas. Therefore, exendin-4 and selenium may exert their antidiabetic effects by increasing GLP-1R, IRS-1, and preproinsulin expression in the pancreas and by increasing the number of pancreatic islets.

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Change history

  • 05 October 2017

    Volume 73 issue 3 was published with an incorrect cover date. Correct is August 2017. The Publisher apologizes for this mistake and all related inconveniences caused by this.

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Authors and Affiliations

  1. Department of Biological Sciences, Faculty of Science, Beirut Arab University, Beirut, Lebanon

    Ghinwa Barakat & Mohamed E Moustafa

  2. Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt

    Mohamed E Moustafa

  3. Department of Pathology Faculty of Medicine, American University of Beirut, Beirut, Lebanon

    Ibrahim Khalifeh

  4. Department of Natural Sciences, Lebanese American University, Beirut, 1102-2801, Lebanon

    Mohammad H Hodroj & Sandra Rizk

  5. Department of Physiology Faculty of Medicine, American University of Beirut, Beirut, Lebanon

    Anwar Bikhazi

Authors
  1. Ghinwa Barakat
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  2. Mohamed E Moustafa
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  3. Ibrahim Khalifeh
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  4. Mohammad H Hodroj
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  5. Anwar Bikhazi
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  6. Sandra Rizk
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Contributions

SR, MEM, and AB conceived, designed the study, and oversaw the analysis and interpretation of data. GB performed all animal experiments and drafted the manuscript. GB and MH designed and carried out western blots and PCR experiments. IK performed the immunofluorescence experiments and the hematoxylin/eosin staining. MEM and SR secured the funding for the project. All the authors read and approved the final manuscript.

Corresponding author

Correspondence to Sandra Rizk.

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Funding

This work was supported by the research fund from the National Council for Scientific Research (CNRS), Lebanon to M.E.M. (grant number 01-11-08) and by the School Research and Development Council, Lebanese American University (SRDC-t-2016-4).

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

An erratum to this article is available at https://doi.org/10.1007/s13105-017-0593-x.

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Barakat, G., Moustafa, M.E., Khalifeh, I. et al. Effects of exendin-4 and selenium on the expression of GLP-1R, IRS-1, and preproinsulin in the pancreas of diabetic rats. J Physiol Biochem 73, 387–394 (2016). https://doi.org/10.1007/s13105-017-0565-1

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