Skip to main content

Advertisement

SpringerLink
Log in

Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats

  • Original Paper
  • Published:
Journal of Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

Pouteria campechiana (Kunth) Baehni. is used as a remedy for coronary trouble, liver disorders, epilepsy, skin disease, and ulcer. Therefore, the present study aims to investigate the antioxidant and hepatoprotective effect of polyphenolic-rich P. campechiana fruit extract against acetaminophen-intoxicated rats. Total phenolic and flavonoid contents of egg fruit were estimated followed by the determination of antioxidant activities. Treatment with P. campechiana fruit extract effectively scavenged the free radicals in a concentration-dependent manner within the range of the given concentrations in all antioxidant models. The presence of polyphenolic compounds were confirmed by high-performance thin-layer chromatography (HPTLC). The animals were treated with acetaminophen (250 mg/kg body weight; p.o.) thrice at the interval of every 5 days after the administration of P. campechiana aqueous extract and silymarin (50 mg/kg). Acetaminophen treatment was found to trigger an oxidative stress in liver, leading to an increase of serum marker enzymes. However, treatment with P. campechiana fruit extract significantly reduced the elevated liver marker enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) and increased the antioxidant enzymes (viz., superoxide dismutase and catalase) and glutathione indicating the effect of the extract in restoring the normal functional ability of hepatocytes. These results strongly suggest that P. campechiana fruit extract has strong antioxidant and significant hepatoprotective effect against acetaminophen-induced hepatotoxicity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Abo Salem OM, Abd-Ellah MF, Ghonaim MM (2011) Hepatoprotective activity of quercetin against acrylonitrile-induced hepatotoxicity in rats. J Biochem Mol Toxicol 25(6):386–392

    Article  CAS  PubMed  Google Scholar 

  2. Aebi H (1983) Catalase in vitro. Methods in enzymatic analysis, vol. 3. Academic, New York, pp. 276–286

  3. Balerdi CF, Shaw PE (1998) Sapodilla, sapote and related fruit. In: Shaw PE, Chan HT, Jr., Nagy, S (eds) Tropical and subtropical fruits. AgScience, Inc., Auburndale, FL, pp. 8–136

  4. Bhattacharjee S (2005) Reactive oxygen species and oxidative burst: Roles in stress senescence and signal transduction in plants. Curr Sci 88(9):1113–1121

    Google Scholar 

  5. Bhattacharya HSC, Zhang Wang YJ (2005) Embryonic development of the rosy barb Puntius conchonius Hamilton 1822 (Cyprinidae). Trop Zool 18:25–37

    Article  Google Scholar 

  6. Chan KW, Khong NMH, Iqbal S, Umar IM, Ismail M (2012) Antioxidant property enhancement of sweet potato flour under simulated gastrointestinal pH. Int J Mol Sci 13(7):8987–8997

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Eesha BR, Amberkar MV, Kumari MK, Babu S, Vijay M, Lalit M, Rajput R (2011) Hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatocellular damage in Wistar albino rats. Asian Pac J Trop Med 4(6):466–469

    Article  CAS  PubMed  Google Scholar 

  8. Farghaly HS, Hussein MA (2010) Protective effect of curcumin against paracetamol-induced liver damage. Aust J Basic Appl Sci 4(9):4266–4274

    CAS  Google Scholar 

  9. Girish C, Pradhan SC (2012) Hepatoprotective activities of picroliv, curcumin, and ellagic acid compared to silymarin on carbon-tetrachloride-induced liver toxicity in mice. J Pharmacol Pharmacother 3(2):149–155

    CAS  PubMed Central  PubMed  Google Scholar 

  10. Green LC, Wagner DA, Glogowski J et al (1982) Analysis of nitrate, nitrite and nitrate in biological fluids. Anal Biochem 126:131–138

    Article  CAS  PubMed  Google Scholar 

  11. Gulcin I, Bursal E, Sehitoglu MH, Bilsel M, Goren AC (2010) Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem Toxicol 48:2227–2238

    Article  PubMed  Google Scholar 

  12. Halliwell B (1997) Antioxidants and human disease: a general introduction. Nutr Rev 55:44–49

    Article  Google Scholar 

  13. He M, Zhang S, Jiao Y, Lin X, Huang J, Chen C, Chen Z, Huang R (2012) Effects and mechanisms of rifampin on hepatotoxicity of acetaminophen in mice. Food Chem Toxicol 50:3142–3149

    Article  CAS  PubMed  Google Scholar 

  14. Oz HS, McClain CJ, Nagasawa HT, Ray MB, de Villiers WJ, Chen TS (2004) Diverse antioxidants protect against acetaminophen hepatotoxicity. J Biochem Mol Toxic 18(6):361–368

    Article  CAS  Google Scholar 

  15. James P, Mayeux PR, Hinson JA (2003) Acetaminophen-induced hepatotoxicity. Drug Metab Dispos 31(12):1499–1506

    Article  CAS  PubMed  Google Scholar 

  16. Jeyadevi R, Sivasudha T, Rameshkumar A, Harnly JM, Lin LZ (2013) Phenolic profiling by UPLC-MS/MS and hepatoprotective activity of Cardiospermum helicacabum against acetaminophen induced liver injury in Wistar rats. Journal Funct Foods 5:289–298

    Article  CAS  Google Scholar 

  17. Johnkennedy N, Adamma E (2011) The protective role of Gongronema latifolium in acetaminophen induced hepatic toxicity in Wistar rats. Asian Pac J Trop Biomed 1:S151–S154

    Google Scholar 

  18. Kakkar P, Das B, Viswanathan PN (1984) A modified spectrophotometric assay of SOD. Indian J Biochem Biophys 21:130–132

    CAS  PubMed  Google Scholar 

  19. Karaman S, Tutem E, Baskan KS, Apak R (2010) Comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC-CUPRAC assay. Food Chem 120:1201–1209

    Article  CAS  Google Scholar 

  20. King EJ, Armstrong AR (1980) Calcium, magnesium, phosphorous and phosphatase. In: Varley B, Gowenlock AH, Bell M (eds) Practical clinical biochemistry. Heinemann, London, pp. 850–852

  21. King J (1965) The hydrolases-acid and alkaline phosphatases. In: Van, D (ed), Practical Clinical Enzymology. Nostrand Company Ltd., London, pp. 191–197.

  22. Latha TB, Srikanth A, Kumar EK, Mastan SK, Srinivasa Rao Y, Bhavani B (2009) Comparative hepatoprotective efficacy of kumaryasava and Livfit against carbon tetrachloride induced hepatic damage in rats. Pharmacologyonline 1:1127–1134

    Google Scholar 

  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J BiolChem 193:265

    CAS  Google Scholar 

  24. Ma J, Yang H, Basile MJ, Kennelly EJ (2004) Analysis of polyphenolic antioxidants from the fruits of three Pouteria species by selected ion monitoring liquid chromatography-mass spectrometry. J Agric Food Chem 52:5873–5878

    Article  CAS  PubMed  Google Scholar 

  25. Malloy HT, Evelyn KA (1937) The determination of bilirubin with the photometric colorimeter. J Biol Chem 119:481–490

    CAS  Google Scholar 

  26. Manokaran S, Jaswanth A, Sengottuvelu S, Nandhakumar J, Duraisamy R, Karthikeyan D (2008) Hepatoprotective activity of Aerva lanata Linn. against paracetamol induced hepatotoxicity in rats. Res J Pharm Tech 1:398–400

    Google Scholar 

  27. McGill MR, Sharpe MR, Williams CD, Taha M, Curry SC, Jaeschke H (2012) The mechanism underlying acetaminophen induced hepatotoxicity in humans and mice involves mitochondrial damage and nuclear DNA fragmentation. J Clin Invest 122(4):1547–1583

    Article  Google Scholar 

  28. Moron MS, Depierre JW, Mannervik B (1979) Levels of glutathione, glutathione reductase and glutathione Stransferase activities in rat lung and liver. Biochim Biophys Acta 582:67–78

    Article  CAS  PubMed  Google Scholar 

  29. Morton J (1987) Canistel. In: Julia, F.M. (ed.) Fruits of warm climates. Purdue University, Miami, pp. 402–405

  30. Olaleye MT, Akinmoladun AC, Ogunboye AA, Akindahunsi AA (2010) Antioxidant activity and hepatoprotective property of leaf extracts of Boerhaavia diffusa Linn against acetaminophen-induced liver damage in rats. Food Chem Toxicol 48:2200–2205

    Article  CAS  PubMed  Google Scholar 

  31. Ordonez AAL, Gomez JD, Vattuone MA, Islamic MI (2006) Antioxidant activities of Sechium edule (Jacq.) swartz extracts. Food Chem 97:452–458

    Article  CAS  Google Scholar 

  32. Orhan IE, Senera B, Musharraf SG (2012) Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Exp Toxicol Pathol 64:205–209

    Article  CAS  PubMed  Google Scholar 

  33. Oyaizu M (1986) Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 44:307–315

    Article  CAS  Google Scholar 

  34. Parmar SR, Vashrambhai PH, Kalia K (2010) Hepatoprotective activity of some plants extract against paracetamol induced hepatotoxicity in rats. J Herb Med Toxicol 4(2):101–106

    CAS  Google Scholar 

  35. Rang HP, Dale MM, Ritter JM, Moore PK (2003) Pharmacology, 5th edn. Bath, Churchill Livingstone

    Google Scholar 

  36. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  CAS  PubMed  Google Scholar 

  37. Reid AB, Kurten RC, McCullough SS, Brock RW, Hinson JA (2005) Mechanisms of acetaminophen-induced hepatotoxicity: role of oxidative stress and mitochondrial permeability transition in freshly isolated mouse hepatocytes. J Pharm Exp Ther 312(2):509–516

    Article  CAS  Google Scholar 

  38. Reitman S, Frankel S (1957) Determination of serum glutamate oxaloacetate and glutamic pyruvic acid transaminases. Amer J Clin Pathol 28:56–63

    CAS  Google Scholar 

  39. Sabir SM, Ahmad SD, Hamid A, Khan MQ, Athayde ML, Santos DB, Boligon AA, Rocha JBT (2012) Antioxidant and hepatoprotective activity of ethanolic extract of leaves of Solidago microglossa containing polyphenolic compounds. Food Chem 131:741–747

    Article  CAS  Google Scholar 

  40. Sadasivam S, Manickam A (1996) Biochemical methods. New age international (P) Limited, Publishers. New Delhi 8:193–194

    Google Scholar 

  41. Siddhuraju P, Becker K (2007) The antioxidant and free radical scavenging activities of rocessed cowpea (Vigna unguiculata) seed extracts. Food Chem 101:10–19

    Article  CAS  Google Scholar 

  42. Sindhu ER, Firdous AP, Preethi KC, Kuttan R (2010) Carotenoid lutein protects rats from paracetamol, carbon tetrachloride and ethanol induced hepatic damage. J Pharm Pharmacol 62(8):1054–1060

    Article  CAS  PubMed  Google Scholar 

  43. Singh RP, Murthy CKN, Jayaprakasha GK (2002) Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agri Food Chem 50(1):81–86

    Article  CAS  Google Scholar 

  44. Smilin Bell Aseervatham G, Shamna R, Sangeetha B, Sasikumar JM (2012) In vivo antioxidant activity of bark extract of Bixa orellana L. against acetaminophen-induced oxidative stress. Asian Pac J Trop Biomed s700–s705

  45. Teepica PDD, Maheshu V, Vishnupriya M, Sasikumar JM (2012) In vitro antioxidant activity of banana (Musa spp. ABB cv. Pisang Awak). Indian J Biochem Biophys 49:124–129

    Google Scholar 

  46. Yang J, Li Y, Wang F, Wu C (2010) Hepatoprotective effects of apple polyphenols on CCl4 induced acute liver damage in mice. J Agri Food Chem 58:6525–6531

    Article  CAS  Google Scholar 

  47. You Y, Yoo S, Yoon HG, Park J, Lee YH, Kim S, Oh KT, Lee J, Cho HY, Jun W (2010) In vitro and in vivo hepatoprotective effects of the aqueous extract from Taraxacum officinale (dandelion) root against alcohol-induced oxidative stress. Food Chem Toxicol 48:1632–1637

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the encouragement by Karpagam University, Coimbatore for providing necessary facilities to carry out this research work. Help in the statistical analysis from Dr. V. Maheshu, Karpagam University is acknowledged. The authors are thankful to the Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, Tamilnadu for supporting this study. The authors are also grateful to Dalmia Research Foundation, Coimbatore, India for the HPTLC analysis.

Author information

Authors and Affiliations

  1. Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    G Smilin Bell Aseervatham, T. Sivasudha, R. Jeyadevi & D. Arul Ananth

  2. Department of Biology, Eritrea Institute of Technology, Mai Nefhi, Asmara, Eritrea, Northeast Africa

    J. M. Sasikumar

  3. Department of Biotechnology, Karpagam University, Coimabatore, Tamil Nadu, India

    P. Hephzibah Christabel

Authors
  1. G Smilin Bell Aseervatham
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Sivasudha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. Sasikumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Hephzibah Christabel
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Jeyadevi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. Arul Ananth
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Sivasudha.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aseervatham, G.S.B., Sivasudha, T., Sasikumar, J.M. et al. Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats. J Physiol Biochem 70, 1–14 (2014). https://doi.org/10.1007/s13105-013-0274-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13105-013-0274-3

Keywords

Search

Navigation